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The map label for this gene is yurR [H]

Identifier: 23098470

GI number: 23098470

Start: 1058173

End: 1059300

Strand: Reverse

Name: yurR [H]

Synonym: OB1015

Alternate gene names: 23098470

Gene position: 1059300-1058173 (Counterclockwise)

Preceding gene: 23098474

Following gene: 23098458

Centisome position: 29.18

GC content: 40.43

Gene sequence:

>1128_bases
ATGAGTAAAAAAGTAATTGTTATAGGTGGCGGAATTGTAGGAGCGAGTACTGCTTATTACCTTGCTAAACAAGGAGCTGC
CGTTACACTAATCGATCGTAATGATCAAGGACAAGCTACTGATGCTGCGGCAGGTATCATATGCCCCTGGATTTCACAAC
GCCGTAATAAAGATTGGTATCAACTAGCCAAAAGTGGCGCTGCATTATATACAAAACTGATTGCTGAATTAAAATCCTTA
GGAGAGACAAATACCGGGTATGAAAAAGTCGGCGCAATTAGTCTACATACTGAAACAAAGAAATTAGAGGGTATGGTAGA
ACGAGCAACAAAAAGAAAAGAAACGGCTCCTGAAATTGGTGAAATTTCTACGTTAAACGAATCAGAAACTTTAAACCGCG
TGCCTATTCTTAAAGAGGGATATCGATCTGTCTATATAAGTGGAGCTGCTCGAGTAAATGGGCAAGAGTTACTTCAAGCA
CTTAAAAGAGCTGGAAAATTAAAAGGCGTAAAAATTATAGAAGGCGACGCAAGCTTAAGTATCGAGGATAATCAAATCAA
GGGAGTCCATGTGGATAATCAGTATACCCTCCCTGCCGACAAAGTGATACTTGCAAACGGGGCATGGATGTCCGAACTAG
TTAAACCTTTCGGGATTGATTTACAAGTGTTTCCTCAACGAGCACAAATTACACATGTAAAATTACCTGGGGTCGATACG
ACAAAATGGCCTGTCATTATGCCACCCGGAAATCAGTATATGCTCTCTTTGCCAGATAACCGAATTGTCTTAGGTTCGAC
TTATGAAGATAATGTTGGCTTTGATTATCGAATTACAGCTGGAAAAGTGCATGAAATATTAGATAAAGCATTTAATATCG
CTCCAGAATTAGAAAATAGCACAGTAGTTGAAACAAAAGTTGGATTTAGGCCATTCACCCCTGGCTCCCTCCCAATTGTC
GGCCCACTACATCAAATTAAAGGTGTATATTTGGCTAACGGATTGGGTGGAACAGGGTTAACAATGGGTCCGTTTGTGGG
TCAGCAACTTGCGAAGATAGCACTTGGTGAGTTACCGGATATCGAGCTGGAAAATTATGATGTATCTAAAGCGATAGCTT
CACAATAA

Upstream 100 bases:

>100_bases
ACTATTGGAGGGTTATGGTGAGAAATAAACAATTTAATCTAAAACGCATTGTATTATTGAACGAAATAATTAATCAACAA
TCTGATTTCTCATCGAACAA

Downstream 100 bases:

>100_bases
TATGATTGAAGAGGTGCTAGGATGAAAGGTTCACATATTGTCTTTATCATCATTGTCGTTATATTATTAACCGGTTTTTT
AACTTGGCTATGGTTACAAA

Product: iminodiacetate oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSKKVIVIGGGIVGASTAYYLAKQGAAVTLIDRNDQGQATDAAAGIICPWISQRRNKDWYQLAKSGAALYTKLIAELKSL
GETNTGYEKVGAISLHTETKKLEGMVERATKRKETAPEIGEISTLNESETLNRVPILKEGYRSVYISGAARVNGQELLQA
LKRAGKLKGVKIIEGDASLSIEDNQIKGVHVDNQYTLPADKVILANGAWMSELVKPFGIDLQVFPQRAQITHVKLPGVDT
TKWPVIMPPGNQYMLSLPDNRIVLGSTYEDNVGFDYRITAGKVHEILDKAFNIAPELENSTVVETKVGFRPFTPGSLPIV
GPLHQIKGVYLANGLGGTGLTMGPFVGQQLAKIALGELPDIELENYDVSKAIASQ

Sequences:

>Translated_375_residues
MSKKVIVIGGGIVGASTAYYLAKQGAAVTLIDRNDQGQATDAAAGIICPWISQRRNKDWYQLAKSGAALYTKLIAELKSL
GETNTGYEKVGAISLHTETKKLEGMVERATKRKETAPEIGEISTLNESETLNRVPILKEGYRSVYISGAARVNGQELLQA
LKRAGKLKGVKIIEGDASLSIEDNQIKGVHVDNQYTLPADKVILANGAWMSELVKPFGIDLQVFPQRAQITHVKLPGVDT
TKWPVIMPPGNQYMLSLPDNRIVLGSTYEDNVGFDYRITAGKVHEILDKAFNIAPELENSTVVETKVGFRPFTPGSLPIV
GPLHQIKGVYLANGLGGTGLTMGPFVGQQLAKIALGELPDIELENYDVSKAIASQ
>Mature_374_residues
SKKVIVIGGGIVGASTAYYLAKQGAAVTLIDRNDQGQATDAAAGIICPWISQRRNKDWYQLAKSGAALYTKLIAELKSLG
ETNTGYEKVGAISLHTETKKLEGMVERATKRKETAPEIGEISTLNESETLNRVPILKEGYRSVYISGAARVNGQELLQAL
KRAGKLKGVKIIEGDASLSIEDNQIKGVHVDNQYTLPADKVILANGAWMSELVKPFGIDLQVFPQRAQITHVKLPGVDTT
KWPVIMPPGNQYMLSLPDNRIVLGSTYEDNVGFDYRITAGKVHEILDKAFNIAPELENSTVVETKVGFRPFTPGSLPIVG
PLHQIKGVYLANGLGGTGLTMGPFVGQQLAKIALGELPDIELENYDVSKAIASQ

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=395, Percent_Identity=22.0253164556962, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI21361378, Length=395, Percent_Identity=22.0253164556962, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787438, Length=423, Percent_Identity=25.0591016548463, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40540; Mature: 40409

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVIVIGGGIVGASTAYYLAKQGAAVTLIDRNDQGQATDAAAGIICPWISQRRNKDWY
CCCEEEEEECCHHHCCHHHEEECCCCEEEEEECCCCCCCCHHHCCEECCCHHHHCCCHHH
QLAKSGAALYTKLIAELKSLGETNTGYEKVGAISLHTETKKLEGMVERATKRKETAPEIG
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
EISTLNESETLNRVPILKEGYRSVYISGAARVNGQELLQALKRAGKLKGVKIIEGDASLS
CEECCCCCHHHCCCCCHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEE
IEDNQIKGVHVDNQYTLPADKVILANGAWMSELVKPFGIDLQVFPQRAQITHVKLPGVDT
EECCEEEEEEECCEEECCCCCEEEECCCHHHHHHHHHCCCEEEECCCCEEEEEECCCCCC
TKWPVIMPPGNQYMLSLPDNRIVLGSTYEDNVGFDYRITAGKVHEILDKAFNIAPELENS
CCCCEEECCCCCEEEECCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
TVVETKVGFRPFTPGSLPIVGPLHQIKGVYLANGLGGTGLTMGPFVGQQLAKIALGELPD
EEEEEECCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
IELENYDVSKAIASQ
CEECCCCHHHHHHCC
>Mature Secondary Structure 
SKKVIVIGGGIVGASTAYYLAKQGAAVTLIDRNDQGQATDAAAGIICPWISQRRNKDWY
CCEEEEEECCHHHCCHHHEEECCCCEEEEEECCCCCCCCHHHCCEECCCHHHHCCCHHH
QLAKSGAALYTKLIAELKSLGETNTGYEKVGAISLHTETKKLEGMVERATKRKETAPEIG
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
EISTLNESETLNRVPILKEGYRSVYISGAARVNGQELLQALKRAGKLKGVKIIEGDASLS
CEECCCCCHHHCCCCCHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEE
IEDNQIKGVHVDNQYTLPADKVILANGAWMSELVKPFGIDLQVFPQRAQITHVKLPGVDT
EECCEEEEEEECCEEECCCCCEEEECCCHHHHHHHHHCCCEEEECCCCEEEEEECCCCCC
TKWPVIMPPGNQYMLSLPDNRIVLGSTYEDNVGFDYRITAGKVHEILDKAFNIAPELENS
CCCCEEECCCCCEEEECCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
TVVETKVGFRPFTPGSLPIVGPLHQIKGVYLANGLGGTGLTMGPFVGQQLAKIALGELPD
EEEEEECCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
IELENYDVSKAIASQ
CEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]