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The map label for this gene is hsrA [H]

Identifier: 23098415

GI number: 23098415

Start: 1012402

End: 1013829

Strand: Direct

Name: hsrA [H]

Synonym: OB0960

Alternate gene names: 23098415

Gene position: 1012402-1013829 (Clockwise)

Preceding gene: 23098414

Following gene: 23098416

Centisome position: 27.89

GC content: 35.15

Gene sequence:

>1428_bases
ATGATTGGTTCGTCATTTTTACATTCAAGAAAAGTAGTTTGTTTCATCTATATATTAGCAATGTTTATGGCTTCATTAGA
TGGAACAATTGTATATGTAGCATTACCAACGATTGGAGAAGACTTAAACGTATCATCAGCAGATTTAAGTGGTGTGACGG
TTTGGTATCTTGTAGGTGTTGCTGCGATCCTGCCTATTGCGGGATGGCTTGGAGATCGATTTGGAAGTAAGAAAGTATTT
TTAAGTGCATTGTCTATATTTACGATTGCATCTATTATTTGTGGACTTGTTCAAACAGTAGGCCTACTAAATATCGCACG
TTTTATACAAGGTCTAGGCGGAGGTATGCTTGCTCCTATCGGCATGGCGATGATATTCCGAACTTTTTCAGTAGAAGAAC
GTCCTAAAGTATCTCGTTCTCTTGTTTTACCGATAGCAATTGCCCCAGCATTAGGTCCTATAGTAGGCGGGTTAATCTTA
GAAATTCTTTCTTGGCAATGGATTTTCTTTATCAATATACCAATTGGAGTTATCGCAATTGTTATTGGTATGTTCTATCT
AACCGAACATCAAGAGGAAAATGTAGGGAGAATGGATTGGAAAGGATTTGTTCTCATTGTTGTAGGTTATCCTTTATTAA
TGTTTTCTTTAACACAAGGTTCTTCAAGAGGTTGGACTTCGATTGAAATACTTGGTTCTGCAATTTGTGGATTACTATTA
GTCAGTGTATTTATATTGCATGCAATAAATAAAGAAAAACCTATTATGGATGTAAAGCTATTATCCGAGCCTACATTTCG
TAAGGTAAGTATACTTTCTTTCTTTGCTGTTAGTGGTTTACAAAGCTTACTTTTTATATTTCCAATTATGTATCAACAGG
CAGTAGGTGCAACCGCACTGGAAAGCGGTATTGTTGTATTTATTGAAGCAATTGGATTAATGTTGGCTTCAAGATTAATG
CCGTTTTCGTTAAAAAAGCTAGGCGTCTACCGAGTCATTTTGTTTGGATTTTTAGGCGCATGTATCAGCTTTATACTAAT
GAGCATAGTTGGACCTTTTGCTAATCCTTGGGTGTTAAGAATACTATTATTTACGATCGGATTAACATTAGGACATTCGG
TAATTGCTATGCAATATACGACATTTACGAATATAACTTCTCAAAATATGAGTAAAGCAACGACGTTATTTAATGTTCAA
AATCGTTTAGGAGCAGCGATTGGTATATCAATGATTGCTAGTTTACTTATTACGCTACAAGATGAGGCGGAAAGCATTAT
GGACCCTAATCCGTATCAGATCTCTATTTTCATTGCTGCAATATTTCTAGCAATTTCATTTATACTTACGTTTAAAAACC
GTCATATTATTAAGCATAGTGATAAACAAACAAATCGTAACTCTATTAAAGGAGAAAAAGCGGGGTAA

Upstream 100 bases:

>100_bases
TGGACCAGAAGGATTAACTGTTTGTCAGGACTATATCGAAGACTATTGGAATGATTTACTTCCAACTAGCTTGAAAAAGA
AAAAGCTGAGTATTTCTTAA

Downstream 100 bases:

>100_bases
TGACTATCTATGCTTGTAAATTACCTTCCTATCAAAATTGGCAGGAAGTGCAATTCTACAGCCAATATATTTCACAAAAA
AGAAGAGATAGGATACAGAA

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MIGSSFLHSRKVVCFIYILAMFMASLDGTIVYVALPTIGEDLNVSSADLSGVTVWYLVGVAAILPIAGWLGDRFGSKKVF
LSALSIFTIASIICGLVQTVGLLNIARFIQGLGGGMLAPIGMAMIFRTFSVEERPKVSRSLVLPIAIAPALGPIVGGLIL
EILSWQWIFFINIPIGVIAIVIGMFYLTEHQEENVGRMDWKGFVLIVVGYPLLMFSLTQGSSRGWTSIEILGSAICGLLL
VSVFILHAINKEKPIMDVKLLSEPTFRKVSILSFFAVSGLQSLLFIFPIMYQQAVGATALESGIVVFIEAIGLMLASRLM
PFSLKKLGVYRVILFGFLGACISFILMSIVGPFANPWVLRILLFTIGLTLGHSVIAMQYTTFTNITSQNMSKATTLFNVQ
NRLGAAIGISMIASLLITLQDEAESIMDPNPYQISIFIAAIFLAISFILTFKNRHIIKHSDKQTNRNSIKGEKAG

Sequences:

>Translated_475_residues
MIGSSFLHSRKVVCFIYILAMFMASLDGTIVYVALPTIGEDLNVSSADLSGVTVWYLVGVAAILPIAGWLGDRFGSKKVF
LSALSIFTIASIICGLVQTVGLLNIARFIQGLGGGMLAPIGMAMIFRTFSVEERPKVSRSLVLPIAIAPALGPIVGGLIL
EILSWQWIFFINIPIGVIAIVIGMFYLTEHQEENVGRMDWKGFVLIVVGYPLLMFSLTQGSSRGWTSIEILGSAICGLLL
VSVFILHAINKEKPIMDVKLLSEPTFRKVSILSFFAVSGLQSLLFIFPIMYQQAVGATALESGIVVFIEAIGLMLASRLM
PFSLKKLGVYRVILFGFLGACISFILMSIVGPFANPWVLRILLFTIGLTLGHSVIAMQYTTFTNITSQNMSKATTLFNVQ
NRLGAAIGISMIASLLITLQDEAESIMDPNPYQISIFIAAIFLAISFILTFKNRHIIKHSDKQTNRNSIKGEKAG
>Mature_475_residues
MIGSSFLHSRKVVCFIYILAMFMASLDGTIVYVALPTIGEDLNVSSADLSGVTVWYLVGVAAILPIAGWLGDRFGSKKVF
LSALSIFTIASIICGLVQTVGLLNIARFIQGLGGGMLAPIGMAMIFRTFSVEERPKVSRSLVLPIAIAPALGPIVGGLIL
EILSWQWIFFINIPIGVIAIVIGMFYLTEHQEENVGRMDWKGFVLIVVGYPLLMFSLTQGSSRGWTSIEILGSAICGLLL
VSVFILHAINKEKPIMDVKLLSEPTFRKVSILSFFAVSGLQSLLFIFPIMYQQAVGATALESGIVVFIEAIGLMLASRLM
PFSLKKLGVYRVILFGFLGACISFILMSIVGPFANPWVLRILLFTIGLTLGHSVIAMQYTTFTNITSQNMSKATTLFNVQ
NRLGAAIGISMIASLLITLQDEAESIMDPNPYQISIFIAAIFLAISFILTFKNRHIIKHSDKQTNRNSIKGEKAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=477, Percent_Identity=29.559748427673, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788392, Length=425, Percent_Identity=29.4117647058824, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789042, Length=433, Percent_Identity=26.5588914549654, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI87081983, Length=436, Percent_Identity=26.8348623853211, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=27.1186440677966, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790146, Length=191, Percent_Identity=32.9842931937173, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994889, Length=166, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788509, Length=165, Percent_Identity=32.7272727272727, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=417, Percent_Identity=23.5011990407674, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319770, Length=450, Percent_Identity=24.4444444444444, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=332, Percent_Identity=25.9036144578313, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=299, Percent_Identity=26.7558528428094, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=313, Percent_Identity=23.961661341853, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51735; Mature: 51735

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSSFLHSRKVVCFIYILAMFMASLDGTIVYVALPTIGEDLNVSSADLSGVTVWYLVGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
AAILPIAGWLGDRFGSKKVFLSALSIFTIASIICGLVQTVGLLNIARFIQGLGGGMLAPI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GMAMIFRTFSVEERPKVSRSLVLPIAIAPALGPIVGGLILEILSWQWIFFINIPIGVIAI
HHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHH
VIGMFYLTEHQEENVGRMDWKGFVLIVVGYPLLMFSLTQGSSRGWTSIEILGSAICGLLL
HHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VSVFILHAINKEKPIMDVKLLSEPTFRKVSILSFFAVSGLQSLLFIFPIMYQQAVGATAL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ESGIVVFIEAIGLMLASRLMPFSLKKLGVYRVILFGFLGACISFILMSIVGPFANPWVLR
HCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ILLFTIGLTLGHSVIAMQYTTFTNITSQNMSKATTLFNVQNRLGAAIGISMIASLLITLQ
HHHHHHHHHHCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAESIMDPNPYQISIFIAAIFLAISFILTFKNRHIIKHSDKQTNRNSIKGEKAG
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGSSFLHSRKVVCFIYILAMFMASLDGTIVYVALPTIGEDLNVSSADLSGVTVWYLVGV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
AAILPIAGWLGDRFGSKKVFLSALSIFTIASIICGLVQTVGLLNIARFIQGLGGGMLAPI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GMAMIFRTFSVEERPKVSRSLVLPIAIAPALGPIVGGLILEILSWQWIFFINIPIGVIAI
HHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHH
VIGMFYLTEHQEENVGRMDWKGFVLIVVGYPLLMFSLTQGSSRGWTSIEILGSAICGLLL
HHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VSVFILHAINKEKPIMDVKLLSEPTFRKVSILSFFAVSGLQSLLFIFPIMYQQAVGATAL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ESGIVVFIEAIGLMLASRLMPFSLKKLGVYRVILFGFLGACISFILMSIVGPFANPWVLR
HCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ILLFTIGLTLGHSVIAMQYTTFTNITSQNMSKATTLFNVQNRLGAAIGISMIASLLITLQ
HHHHHHHHHHCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAESIMDPNPYQISIFIAAIFLAISFILTFKNRHIIKHSDKQTNRNSIKGEKAG
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]