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The map label for this gene is fruB [H]

Identifier: 23098294

GI number: 23098294

Start: 891020

End: 891931

Strand: Reverse

Name: fruB [H]

Synonym: OB0839

Alternate gene names: 23098294

Gene position: 891931-891020 (Counterclockwise)

Preceding gene: 23098295

Following gene: 23098293

Centisome position: 24.57

GC content: 34.98

Gene sequence:

>912_bases
ATGATTTACACAATAACTCTTAATCCATCGATTGACTTCATTGTACCTGTCGATCAACTGAAACTTGGAAAGCTAAACTA
CATGAACGATGAGTATAAAGTACCTGGTGGCAAGGGAATTAACGTTTCCCGTATCTTACAAGAACTCACAACCTCCTCCA
CCGCATTAGGATTCATTGGTGGTTTTACTGGAAATTTTATTATTGATTTTCTAGAAAAAAGAAATCTTAAAACGGATTTT
ATATCTATAAGCGAAGATACAAGAATCAATATTAAATTGAAATCAAGTAGTGAGACTGAAATTAATGGCCGAGGACCCTC
TATCTCTAGTGATGAGTCATCCAAACTTCTTTCACAATTAGAAAAAATAACTGCAAATGATTTTGTAGTTTTGTCTGGAA
GTAAACCACCTTCGTTACCAGCTGATTTCTATGAAAAGGTTATCAAAAAAGTAACAGAAACTGGTGCCAGATTTGCCATA
GATACTACTGGAGATGCATTAATGCAATCACTTGCTTATCAGCCATTAGTTATTAAACCAAATCAACATGAGCTAGAGGA
TTTATTTGGGGTAACTATTTCCACTAATGAAGATGTCATTAAATACGGAAAGAAACTTCTAAAAAAAGGAGCACAGCATG
TCATTGTTTCTATGGGTGCTGATGGAGCTATTTTGATAACGAATAACGGAGTTTATAAAGGTACTTCTCCAAGTGGGACA
TTACGTAATTCTGTCGGAGCTGGAGACTCTATGATTGCTGGTTTTCTTAGCATTTACTTAACTAGTCAGAACGTACTTGA
GGCATTCAAAATGAGTATTGCTGCAGGAAGTGCCACTGCTTTTTCTCATGATCTAGCAACCAGAAATAAAATTGATAACT
TACTTTCAGAAATTGAAATAACGACATTATAA

Upstream 100 bases:

>100_bases
AAAAATCTGTGATATTAATGAAGTTAACTTTATTATAGAAAAACTGACACCCACACACGAACCTTTTCAACCTCATACTA
ACATCTTGGAGGCAACATAA

Downstream 100 bases:

>100_bases
AACAGAAAGGATGATAGAGATGGACGTAATTGATTTATTACGCAAAGATATTATGATTATGGATTTGAAATCGACGGATA
AGCAATCTGTTATCGAAGAG

Product: fructose 1-phosphate kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIYTITLNPSIDFIVPVDQLKLGKLNYMNDEYKVPGGKGINVSRILQELTTSSTALGFIGGFTGNFIIDFLEKRNLKTDF
ISISEDTRINIKLKSSSETEINGRGPSISSDESSKLLSQLEKITANDFVVLSGSKPPSLPADFYEKVIKKVTETGARFAI
DTTGDALMQSLAYQPLVIKPNQHELEDLFGVTISTNEDVIKYGKKLLKKGAQHVIVSMGADGAILITNNGVYKGTSPSGT
LRNSVGAGDSMIAGFLSIYLTSQNVLEAFKMSIAAGSATAFSHDLATRNKIDNLLSEIEITTL

Sequences:

>Translated_303_residues
MIYTITLNPSIDFIVPVDQLKLGKLNYMNDEYKVPGGKGINVSRILQELTTSSTALGFIGGFTGNFIIDFLEKRNLKTDF
ISISEDTRINIKLKSSSETEINGRGPSISSDESSKLLSQLEKITANDFVVLSGSKPPSLPADFYEKVIKKVTETGARFAI
DTTGDALMQSLAYQPLVIKPNQHELEDLFGVTISTNEDVIKYGKKLLKKGAQHVIVSMGADGAILITNNGVYKGTSPSGT
LRNSVGAGDSMIAGFLSIYLTSQNVLEAFKMSIAAGSATAFSHDLATRNKIDNLLSEIEITTL
>Mature_303_residues
MIYTITLNPSIDFIVPVDQLKLGKLNYMNDEYKVPGGKGINVSRILQELTTSSTALGFIGGFTGNFIIDFLEKRNLKTDF
ISISEDTRINIKLKSSSETEINGRGPSISSDESSKLLSQLEKITANDFVVLSGSKPPSLPADFYEKVIKKVTETGARFAI
DTTGDALMQSLAYQPLVIKPNQHELEDLFGVTISTNEDVIKYGKKLLKKGAQHVIVSMGADGAILITNNGVYKGTSPSGT
LRNSVGAGDSMIAGFLSIYLTSQNVLEAFKMSIAAGSATAFSHDLATRNKIDNLLSEIEITTL

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=297, Percent_Identity=31.3131313131313, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788493, Length=258, Percent_Identity=28.6821705426357, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32756; Mature: 32756

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTITLNPSIDFIVPVDQLKLGKLNYMNDEYKVPGGKGINVSRILQELTTSSTALGFIG
CEEEEEECCCCEEEEECCCEEECCEECCCCCEECCCCCCCCHHHHHHHHHCCCHHHHHHC
GFTGNFIIDFLEKRNLKTDFISISEDTRINIKLKSSSETEINGRGPSISSDESSKLLSQL
CCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHH
EKITANDFVVLSGSKPPSLPADFYEKVIKKVTETGARFAIDTTGDALMQSLAYQPLVIKP
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCEEECC
NQHELEDLFGVTISTNEDVIKYGKKLLKKGAQHVIVSMGADGAILITNNGVYKGTSPSGT
CHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCEECCCCCCCC
LRNSVGAGDSMIAGFLSIYLTSQNVLEAFKMSIAAGSATAFSHDLATRNKIDNLLSEIEI
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEE
TTL
ECC
>Mature Secondary Structure
MIYTITLNPSIDFIVPVDQLKLGKLNYMNDEYKVPGGKGINVSRILQELTTSSTALGFIG
CEEEEEECCCCEEEEECCCEEECCEECCCCCEECCCCCCCCHHHHHHHHHCCCHHHHHHC
GFTGNFIIDFLEKRNLKTDFISISEDTRINIKLKSSSETEINGRGPSISSDESSKLLSQL
CCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHH
EKITANDFVVLSGSKPPSLPADFYEKVIKKVTETGARFAIDTTGDALMQSLAYQPLVIKP
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCEEECC
NQHELEDLFGVTISTNEDVIKYGKKLLKKGAQHVIVSMGADGAILITNNGVYKGTSPSGT
CHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCEECCCCCCCC
LRNSVGAGDSMIAGFLSIYLTSQNVLEAFKMSIAAGSATAFSHDLATRNKIDNLLSEIEI
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEE
TTL
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]