Click here to switch to the map view.

The map label for this gene is yfeW [H]

Identifier: 23098122

GI number: 23098122

Start: 703855

End: 705339

Strand: Direct

Name: yfeW [H]

Synonym: OB0667

Alternate gene names: 23098122

Gene position: 703855-705339 (Clockwise)

Preceding gene: 23098121

Following gene: 23098123

Centisome position: 19.39

GC content: 40.2

Gene sequence:

>1485_bases
ATGAGGAAGAAAGTAATGTCGTTGCTATTTACGTTTATCCTTTTATTTTCCACTTTTATATCAGGCGCTACTGGTATTTA
CGCTTCAGAGAGTGGATTAAATCCAGGAGATGCTCATGATGTTGAAATGCTGCAACAGCCATTAGATGATCTAGATCCAT
TTATTGAGAATGCAATATCTGATCAGGTGATGCCTGGTGCTGTTGTTCTAGTCGCTCGAGATGGACAGATTGTTAAACAT
GATGCATATGGCTATGCTGCGCGCTATACGGATGGTAATTTTACCGAAATGGAAAATCCAATTGAAATGCAGGAAAATAC
CATCTTTGATACAGCGTCTATTTCAAAGCTTTTTACAGCAACAGCAATTATGACTCTTTGGGATGAAGGATTGTTTGAAT
TGGATGATCCGGTAGCTTTATATATTCCTGAGTTTGCAAGTAATGGGAAAGAAGAAGTCACAATCAAGCAATTATTAACC
CATACATCAGGTTTTCGTGCATCTACTACGGTACCCGTCCATGAAATTGAAGGGGATCGTGAAGATCGTTTGGACTTTGT
CTTAAATGAAACTTTAGAAGTGCCGCCGGGTACTCGTTACCTCTATAGTGATGTCGATTTTATTACACTAGGAGTGTTAA
TCGAACGTCTAAGCGGACAACGTCAAGATGAATACGTGGCAGAAAATATAACAGAACCTCTACAAATGAGTGATACGATG
TATAGTCCACCTGCTTCACTAAAGAACAGAATTGCTGCAACGGAGTATCAAGCCAATGTAAATCGTGGACTTGTTTGGGG
AAGTGTTCATGATGAAAAAGCATGGGCATTGGACGGTGTTGCTGGACATGCCGGGGTTTTTAGTTCGGCAGAGGATTTAG
CCGTGTTCGGACAGATGATGTTAAATGAAGGAAGTTACCAAGGTGAACAGATTTTATCCGAGGAAGCCTTTGACCTCATC
ACTACAAATTGGAATGGTGCCTTCCCAGGCCAAGACCAAGGTTTGGGGTGGGAATTAAATCAAAGTTGGTACATGGATGA
TTTAGCAGAACAGAATACGATAGGGCATACAGGCTATACAGGTACGTCCATCGTGATTAGCCCTAACAAGAAATCGATTA
CGATATTACTAACTAATAGAGTACATCCAACAAGAAATACGGTATCAACAAATCCGACCCGCCGAATGGTGTCGGAGAAG
ACTGCCTCTGCTATCGATGCTTGGAGTGCCGAAAGTATGGCGTCTCTTGTTAATCGTTTGGTTGATCGAGGTGAAATTAC
AGATGTCTCTACTACTCGATCACTTCTTACACACTTAAGAACAGTCTCTCATTTTGAGTCACTTGATCAAGCTCATAAAG
TTCGTAAACACTTAGAAGGTCTTCAATTATTAGTGGATTTTCATAATGAGAATAATTCGATATCAGATAATGCATACGAA
ACGTTAACTACGGATATCGATTATTTAACAGGAAAATGGCAATAA

Upstream 100 bases:

>100_bases
TGGTAGTAATTCACTTCATGTAGAAGCAGTAGAACAAGCCTATAATGCAGTTGGAGTCTATTAATCTAATTTTAAAGACT
TTTTAAAAGGGGAGTAGATG

Downstream 100 bases:

>100_bases
AACTAGTAATCTATCATTTAATTTTGTATATCTAAAAAGGAGGATGGATACAATGAATTATTTTAATTGGAAGAAACGTT
TGTCACAGCTTGTCATAGTT

Product: beta-lactamase

Products: substituted beta-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRKKVMSLLFTFILLFSTFISGATGIYASESGLNPGDAHDVEMLQQPLDDLDPFIENAISDQVMPGAVVLVARDGQIVKH
DAYGYAARYTDGNFTEMENPIEMQENTIFDTASISKLFTATAIMTLWDEGLFELDDPVALYIPEFASNGKEEVTIKQLLT
HTSGFRASTTVPVHEIEGDREDRLDFVLNETLEVPPGTRYLYSDVDFITLGVLIERLSGQRQDEYVAENITEPLQMSDTM
YSPPASLKNRIAATEYQANVNRGLVWGSVHDEKAWALDGVAGHAGVFSSAEDLAVFGQMMLNEGSYQGEQILSEEAFDLI
TTNWNGAFPGQDQGLGWELNQSWYMDDLAEQNTIGHTGYTGTSIVISPNKKSITILLTNRVHPTRNTVSTNPTRRMVSEK
TASAIDAWSAESMASLVNRLVDRGEITDVSTTRSLLTHLRTVSHFESLDQAHKVRKHLEGLQLLVDFHNENNSISDNAYE
TLTTDIDYLTGKWQ

Sequences:

>Translated_494_residues
MRKKVMSLLFTFILLFSTFISGATGIYASESGLNPGDAHDVEMLQQPLDDLDPFIENAISDQVMPGAVVLVARDGQIVKH
DAYGYAARYTDGNFTEMENPIEMQENTIFDTASISKLFTATAIMTLWDEGLFELDDPVALYIPEFASNGKEEVTIKQLLT
HTSGFRASTTVPVHEIEGDREDRLDFVLNETLEVPPGTRYLYSDVDFITLGVLIERLSGQRQDEYVAENITEPLQMSDTM
YSPPASLKNRIAATEYQANVNRGLVWGSVHDEKAWALDGVAGHAGVFSSAEDLAVFGQMMLNEGSYQGEQILSEEAFDLI
TTNWNGAFPGQDQGLGWELNQSWYMDDLAEQNTIGHTGYTGTSIVISPNKKSITILLTNRVHPTRNTVSTNPTRRMVSEK
TASAIDAWSAESMASLVNRLVDRGEITDVSTTRSLLTHLRTVSHFESLDQAHKVRKHLEGLQLLVDFHNENNSISDNAYE
TLTTDIDYLTGKWQ
>Mature_494_residues
MRKKVMSLLFTFILLFSTFISGATGIYASESGLNPGDAHDVEMLQQPLDDLDPFIENAISDQVMPGAVVLVARDGQIVKH
DAYGYAARYTDGNFTEMENPIEMQENTIFDTASISKLFTATAIMTLWDEGLFELDDPVALYIPEFASNGKEEVTIKQLLT
HTSGFRASTTVPVHEIEGDREDRLDFVLNETLEVPPGTRYLYSDVDFITLGVLIERLSGQRQDEYVAENITEPLQMSDTM
YSPPASLKNRIAATEYQANVNRGLVWGSVHDEKAWALDGVAGHAGVFSSAEDLAVFGQMMLNEGSYQGEQILSEEAFDLI
TTNWNGAFPGQDQGLGWELNQSWYMDDLAEQNTIGHTGYTGTSIVISPNKKSITILLTNRVHPTRNTVSTNPTRRMVSEK
TASAIDAWSAESMASLVNRLVDRGEITDVSTTRSLLTHLRTVSHFESLDQAHKVRKHLEGLQLLVDFHNENNSISDNAYE
TLTTDIDYLTGKWQ

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0214 family [H]

Homologues:

Organism=Homo sapiens, GI239747261, Length=332, Percent_Identity=24.0963855421687, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239752721, Length=332, Percent_Identity=24.0963855421687, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239741085, Length=332, Percent_Identity=24.0963855421687, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI26051231, Length=113, Percent_Identity=38.0530973451327, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI26051233, Length=113, Percent_Identity=38.0530973451327, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87082101, Length=418, Percent_Identity=34.9282296650718, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786574, Length=310, Percent_Identity=26.1290322580645, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790593, Length=291, Percent_Identity=27.4914089347079, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17550934, Length=300, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533026, Length=191, Percent_Identity=25.6544502617801, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR022849 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6

Molecular weight: Translated: 54945; Mature: 54945

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVMSLLFTFILLFSTFISGATGIYASESGLNPGDAHDVEMLQQPLDDLDPFIENAIS
CHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DQVMPGAVVLVARDGQIVKHDAYGYAARYTDGNFTEMENPIEMQENTIFDTASISKLFTA
CCCCCCEEEEEECCCEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCEECHHHHHHHHHH
TAIMTLWDEGLFELDDPVALYIPEFASNGKEEVTIKQLLTHTSGFRASTTVPVHEIEGDR
HHHHHHHHCCCEECCCCEEEEECHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCH
EDRLDFVLNETLEVPPGTRYLYSDVDFITLGVLIERLSGQRQDEYVAENITEPLQMSDTM
HHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCC
YSPPASLKNRIAATEYQANVNRGLVWGSVHDEKAWALDGVAGHAGVFSSAEDLAVFGQMM
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHH
LNEGSYQGEQILSEEAFDLITTNWNGAFPGQDQGLGWELNQSWYMDDLAEQNTIGHTGYT
HCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCC
GTSIVISPNKKSITILLTNRVHPTRNTVSTNPTRRMVSEKTASAIDAWSAESMASLVNRL
CCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VDRGEITDVSTTRSLLTHLRTVSHFESLDQAHKVRKHLEGLQLLVDFHNENNSISDNAYE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
TLTTDIDYLTGKWQ
HHHCCHHHHCCCCC
>Mature Secondary Structure
MRKKVMSLLFTFILLFSTFISGATGIYASESGLNPGDAHDVEMLQQPLDDLDPFIENAIS
CHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DQVMPGAVVLVARDGQIVKHDAYGYAARYTDGNFTEMENPIEMQENTIFDTASISKLFTA
CCCCCCEEEEEECCCEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCEECHHHHHHHHHH
TAIMTLWDEGLFELDDPVALYIPEFASNGKEEVTIKQLLTHTSGFRASTTVPVHEIEGDR
HHHHHHHHCCCEECCCCEEEEECHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCH
EDRLDFVLNETLEVPPGTRYLYSDVDFITLGVLIERLSGQRQDEYVAENITEPLQMSDTM
HHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCC
YSPPASLKNRIAATEYQANVNRGLVWGSVHDEKAWALDGVAGHAGVFSSAEDLAVFGQMM
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHH
LNEGSYQGEQILSEEAFDLITTNWNGAFPGQDQGLGWELNQSWYMDDLAEQNTIGHTGYT
HCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCC
GTSIVISPNKKSITILLTNRVHPTRNTVSTNPTRRMVSEKTASAIDAWSAESMASLVNRL
CCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VDRGEITDVSTTRSLLTHLRTVSHFESLDQAHKVRKHLEGLQLLVDFHNENNSISDNAYE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
TLTTDIDYLTGKWQ
HHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]