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The map label for this gene is cwlK [H]

Identifier: 23098017

GI number: 23098017

Start: 592470

End: 593030

Strand: Reverse

Name: cwlK [H]

Synonym: OB0562

Alternate gene names: 23098017

Gene position: 593030-592470 (Counterclockwise)

Preceding gene: 23098025

Following gene: 23098015

Centisome position: 16.33

GC content: 38.86

Gene sequence:

>561_bases
ATGAGAGGCTTGACACACTTTATAACTACTTGGGTGCCCATTATCCTTTTTGTAGCTATTGTTTTTATGATTTATCAGGA
AGCAAATCAACCCGAAACTATTTATGTGGGGGAAGGAGCAACTGAAGCTTCTGAACTTCACCCCGTAGTGGAAAAAGCTA
AAAACGAACTGGTTGATCAAGCTTCTAAGAAAGATATCGATGTCATGATAACGGATGAAGTCCGCTCCAAAGAGAGACAG
GATGAACTTTATGCAAGAGGACGAACCACAGAAGGAACCGTTGTAACACATGCTCAAGGTGGAGAAAGCTATCATAATTA
TGGACTAGCAATTGATTATGCTATTAAAAATAATAACGGCAATGTGCTTTGGGATATACACTACGATGGTAATAACAATG
GTGAATCCGACTGGTTTGAAGTAGCTGAAATTGCTAAAGAAATTGGTTTTGAATGGGGAGGCGATTGGCAGCACTTTAAG
GATTATCCTCATCTTCAAATGACATTCGGATTAAGTATAAATGAACTTCAAAAAGGCTATAGGCCGCCTCCAGATAAATA
A

Upstream 100 bases:

>100_bases
ACAATTTTTCTTCAATAGGTTTAATTTAATTATTCTACAAGTCTATTCATGTTATTCTTTTAGAGACATTCAATTTGTCA
AAAAAGGAGTAATAAGCTTA

Downstream 100 bases:

>100_bases
AGAACCATTCATGTTTACTTGAACATCTTTAAGTTGGTATCATGAATGGTTTTGTTTTTTAATCGTTATTCGTTTTTTTC
TCCTTCTCATCATTTTCTTC

Product: L-alanoyl-D-glutamate peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MRGLTHFITTWVPIILFVAIVFMIYQEANQPETIYVGEGATEASELHPVVEKAKNELVDQASKKDIDVMITDEVRSKERQ
DELYARGRTTEGTVVTHAQGGESYHNYGLAIDYAIKNNNGNVLWDIHYDGNNNGESDWFEVAEIAKEIGFEWGGDWQHFK
DYPHLQMTFGLSINELQKGYRPPPDK

Sequences:

>Translated_186_residues
MRGLTHFITTWVPIILFVAIVFMIYQEANQPETIYVGEGATEASELHPVVEKAKNELVDQASKKDIDVMITDEVRSKERQ
DELYARGRTTEGTVVTHAQGGESYHNYGLAIDYAIKNNNGNVLWDIHYDGNNNGESDWFEVAEIAKEIGFEWGGDWQHFK
DYPHLQMTFGLSINELQKGYRPPPDK
>Mature_186_residues
MRGLTHFITTWVPIILFVAIVFMIYQEANQPETIYVGEGATEASELHPVVEKAKNELVDQASKKDIDVMITDEVRSKERQ
DELYARGRTTEGTVVTHAQGGESYHNYGLAIDYAIKNNNGNVLWDIHYDGNNNGESDWFEVAEIAKEIGFEWGGDWQHFK
DYPHLQMTFGLSINELQKGYRPPPDK

Specific function: Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR012340
- InterPro:   IPR003709 [H]

Pfam domain/function: PF02557 VanY [H]

EC number: NA

Molecular weight: Translated: 21222; Mature: 21222

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLTHFITTWVPIILFVAIVFMIYQEANQPETIYVGEGATEASELHPVVEKAKNELVDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ASKKDIDVMITDEVRSKERQDELYARGRTTEGTVVTHAQGGESYHNYGLAIDYAIKNNNG
HCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCC
NVLWDIHYDGNNNGESDWFEVAEIAKEIGFEWGGDWQHFKDYPHLQMTFGLSINELQKGY
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCHHHHHHCC
RPPPDK
CCCCCC
>Mature Secondary Structure
MRGLTHFITTWVPIILFVAIVFMIYQEANQPETIYVGEGATEASELHPVVEKAKNELVDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ASKKDIDVMITDEVRSKERQDELYARGRTTEGTVVTHAQGGESYHNYGLAIDYAIKNNNG
HCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCCC
NVLWDIHYDGNNNGESDWFEVAEIAKEIGFEWGGDWQHFKDYPHLQMTFGLSINELQKGY
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCHHHHHHCC
RPPPDK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]