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The map label for this gene is hisH

Identifier: 23098004

GI number: 23098004

Start: 581488

End: 582093

Strand: Reverse

Name: hisH

Synonym: OB0549

Alternate gene names: 23098004

Gene position: 582093-581488 (Counterclockwise)

Preceding gene: 23098005

Following gene: 23098003

Centisome position: 16.03

GC content: 36.14

Gene sequence:

>606_bases
TTGATTGCAATTATTGATTATGGCGCTGGAAATATAAAAAGTCTTCAATTTGCATTAGATAAATTGAATAAACATTCCAT
TGTTACAACAGAAGCAGCAGAAATCAAACAAGCAGATTCAATTATTTTACCTGGTGTCGGAGCATTCAAAGATGCAATGG
AAGCAATCCGTCAGCTTCAATTAGATTCCGTTATACAAGAGGAAGCGCAAAAAGGAAAACCAATATTAGGGATTTGCCTA
GGTATGCAACTCTTTTATGAACAAAGCCTTGAAAACGGAGATTGGGAAGGATTAGGACTGCTAAAAGGATCCATTAAACG
AATCTCTGGTGAAGTTAAAGTACCACATATGGGTTGGAATACATTAGACATTCAACAAGCTTCGCCACTATTTGATTCTA
AGTTAGAAAATCCTTATGTCTATTTCGTTCACTCCTATGCAGTTTCTTCTTTCGAGGAGAATACTTTACTTGCTAGTTCT
CAATATGGACAGCTAATTCCAGCTATCGTTCAAAAAGGGAATATAACCGGTATGCAATTTCATCCAGAGAAGAGCGGCGA
GTTTGGAATTGAATTACTAAAAAGATATGAGGAGATGATTAGATGA

Upstream 100 bases:

>100_bases
ATTTAAAGGATTCGGGCGTGTGATCCGTCAAGGTTGTTCCATCGACCTAAACCAAAAAGGAATACCTTCAACAAAAGGAA
GTTTATAGGGGGCAATAGCA

Downstream 100 bases:

>100_bases
TTATCTTTCCAGCAATTGATATACGAAACGGGAAATGCGTACGTTTAAGGCAAGGTGACTATAATCAAGAAACTATATAT
AGCAACTCTCCTGTAGAAAT

Product: amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIAIIDYGAGNIKSLQFALDKLNKHSIVTTEAAEIKQADSIILPGVGAFKDAMEAIRQLQLDSVIQEEAQKGKPILGICL
GMQLFYEQSLENGDWEGLGLLKGSIKRISGEVKVPHMGWNTLDIQQASPLFDSKLENPYVYFVHSYAVSSFEENTLLASS
QYGQLIPAIVQKGNITGMQFHPEKSGEFGIELLKRYEEMIR

Sequences:

>Translated_201_residues
MIAIIDYGAGNIKSLQFALDKLNKHSIVTTEAAEIKQADSIILPGVGAFKDAMEAIRQLQLDSVIQEEAQKGKPILGICL
GMQLFYEQSLENGDWEGLGLLKGSIKRISGEVKVPHMGWNTLDIQQASPLFDSKLENPYVYFVHSYAVSSFEENTLLASS
QYGQLIPAIVQKGNITGMQFHPEKSGEFGIELLKRYEEMIR
>Mature_201_residues
MIAIIDYGAGNIKSLQFALDKLNKHSIVTTEAAEIKQADSIILPGVGAFKDAMEAIRQLQLDSVIQEEAQKGKPILGICL
GMQLFYEQSLENGDWEGLGLLKGSIKRISGEVKVPHMGWNTLDIQQASPLFDSKLENPYVYFVHSYAVSSFEENTLLASS
QYGQLIPAIVQKGNITGMQFHPEKSGEFGIELLKRYEEMIR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=36.6834170854271, Blast_Score=131, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=31.4285714285714, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_OCEIH (Q8ESS0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691470.1
- ProteinModelPortal:   Q8ESS0
- SMR:   Q8ESS0
- GeneID:   1015844
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0549
- NMPDR:   fig|221109.1.peg.552
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- BioCyc:   OIHE221109:OB0549-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22322; Mature: 22322

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 181-181 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGAGNIKSLQFALDKLNKHSIVTTEAAEIKQADSIILPGVGAFKDAMEAIRQLQ
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHHCCCCEEECCCHHHHHHHHHHHHHH
LDSVIQEEAQKGKPILGICLGMQLFYEQSLENGDWEGLGLLKGSIKRISGEVKVPHMGWN
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECCCCCCC
TLDIQQASPLFDSKLENPYVYFVHSYAVSSFEENTLLASSQYGQLIPAIVQKGNITGMQF
EEECCCCCCCHHHHCCCCEEEEEEHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCEEE
HPEKSGEFGIELLKRYEEMIR
CCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIAIIDYGAGNIKSLQFALDKLNKHSIVTTEAAEIKQADSIILPGVGAFKDAMEAIRQLQ
CEEEEECCCCCHHHHHHHHHHCCCCCEEEECHHHHHCCCCEEECCCHHHHHHHHHHHHHH
LDSVIQEEAQKGKPILGICLGMQLFYEQSLENGDWEGLGLLKGSIKRISGEVKVPHMGWN
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECCCCCCC
TLDIQQASPLFDSKLENPYVYFVHSYAVSSFEENTLLASSQYGQLIPAIVQKGNITGMQF
EEECCCCCCCHHHHCCCCEEEEEEHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCEEE
HPEKSGEFGIELLKRYEEMIR
CCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376