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The map label for this gene is thiE

Identifier: 23097927

GI number: 23097927

Start: 501449

End: 502069

Strand: Reverse

Name: thiE

Synonym: OB0472

Alternate gene names: 23097927

Gene position: 502069-501449 (Counterclockwise)

Preceding gene: 23097928

Following gene: 23097926

Centisome position: 13.83

GC content: 35.43

Gene sequence:

>621_bases
ATGAAATTCGATAAACATATGCTTCGAAAGTATTTTATTATGGGTAGTCAAAACTGCCATCGAGACCCACGGGAGATATT
AAAAGAGGCAGCCTCTGCAGGAATTACTGCTTTTCAATACAGAGAAAAGGGTAAGAATTCTTTAACAGGAACTGCAAAAG
TTGAACTCGCAAAAGATTTAAAAGCTATTTGCCATGATTTTCAAATCCCTTTTATTATTAATGATGATGTAGATTTAGCA
AAACAATTGGATGCAGATGGTATTCATATCGGTCAAGATGATCAACCTGTAGAGGTTGTGCGGAAACAGTTCCCAAATAA
GATCATTGGATTATCTATATCTACGAATAATGAATTAAATCAAAGCCCATTAGATCTTGTCGATTACATTGGAGTTGGTC
CAATATTTGATACAAATACGAAAGAGGACGCTAAGACAGCGGTAGGTCTTGAATGGATTCAAAGTTTAAAAAAACAACAT
CCTTCCCTTCCATTAGTTGCAATTGGAGGTATTAACACTACCAATGCACAAGAGATAATTCAAGCTGGAGCTGATGGTGT
ATCTTTTATTTCAGCCATTACAGAAACTGATCATATTTTACAGGCTGTACAACGCTTATAA

Upstream 100 bases:

>100_bases
TAATCAAGAAAATGTTCATGGTACAGGTAGTTTTTTACCTGTATTCATTGATTCGCTTTCTAAAATGCCACAGGAACTTA
CGGAAGGAGAATAAATCATT

Downstream 100 bases:

>100_bases
AGTGAAATTTAACCTAGTGGGGATTTTCATTCTCCTCTGCTTGAATGTTATTTATCCTGTGAACAACTATAACTTTCAGC
TAAGCCTCATAGTTTAAATA

Product: thiamine phosphate synthase chain B

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKFDKHMLRKYFIMGSQNCHRDPREILKEAASAGITAFQYREKGKNSLTGTAKVELAKDLKAICHDFQIPFIINDDVDLA
KQLDADGIHIGQDDQPVEVVRKQFPNKIIGLSISTNNELNQSPLDLVDYIGVGPIFDTNTKEDAKTAVGLEWIQSLKKQH
PSLPLVAIGGINTTNAQEIIQAGADGVSFISAITETDHILQAVQRL

Sequences:

>Translated_206_residues
MKFDKHMLRKYFIMGSQNCHRDPREILKEAASAGITAFQYREKGKNSLTGTAKVELAKDLKAICHDFQIPFIINDDVDLA
KQLDADGIHIGQDDQPVEVVRKQFPNKIIGLSISTNNELNQSPLDLVDYIGVGPIFDTNTKEDAKTAVGLEWIQSLKKQH
PSLPLVAIGGINTTNAQEIIQAGADGVSFISAITETDHILQAVQRL
>Mature_206_residues
MKFDKHMLRKYFIMGSQNCHRDPREILKEAASAGITAFQYREKGKNSLTGTAKVELAKDLKAICHDFQIPFIINDDVDLA
KQLDADGIHIGQDDQPVEVVRKQFPNKIIGLSISTNNELNQSPLDLVDYIGVGPIFDTNTKEDAKTAVGLEWIQSLKKQH
PSLPLVAIGGINTTNAQEIIQAGADGVSFISAITETDHILQAVQRL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=165, Percent_Identity=29.0909090909091, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=193, Percent_Identity=34.7150259067358, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_OCEIH (Q8ESZ3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691393.1
- ProteinModelPortal:   Q8ESZ3
- SMR:   Q8ESZ3
- GeneID:   1015743
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0472
- NMPDR:   fig|221109.1.peg.478
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- BioCyc:   OIHE221109:OB0472-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22735; Mature: 22735

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDKHMLRKYFIMGSQNCHRDPREILKEAASAGITAFQYREKGKNSLTGTAKVELAKDL
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHH
KAICHDFQIPFIINDDVDLAKQLDADGIHIGQDDQPVEVVRKQFPNKIIGLSISTNNELN
HHHHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC
QSPLDLVDYIGVGPIFDTNTKEDAKTAVGLEWIQSLKKQHPSLPLVAIGGINTTNAQEII
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
QAGADGVSFISAITETDHILQAVQRL
HCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFDKHMLRKYFIMGSQNCHRDPREILKEAASAGITAFQYREKGKNSLTGTAKVELAKDL
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHH
KAICHDFQIPFIINDDVDLAKQLDADGIHIGQDDQPVEVVRKQFPNKIIGLSISTNNELN
HHHHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC
QSPLDLVDYIGVGPIFDTNTKEDAKTAVGLEWIQSLKKQHPSLPLVAIGGINTTNAQEII
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
QAGADGVSFISAITETDHILQAVQRL
HCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376