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The map label for this gene is thiM1

Identifier: 23097928

GI number: 23097928

Start: 502076

End: 502849

Strand: Reverse

Name: thiM1

Synonym: OB0473

Alternate gene names: 23097928

Gene position: 502849-502076 (Counterclockwise)

Preceding gene: 23097929

Following gene: 23097927

Centisome position: 13.85

GC content: 37.21

Gene sequence:

>774_bases
ATGCAGGTCGATATTATTAAACAGGTACGTGACACTAAACCACTTGTGCATCACCTAACTAATCAAGTAGTAATGAACTT
CTCTGCCAATGGTTTACTTTCATTTGGCGGTTCACCAATTATGGCAAAAGCGATAGAAGAAGTTGAACAAATTGCGGCAA
TTTCAAATGCCGTTTTAATTAATATCGGTACGTTAACTTCCACCGAATTGGAATCTATGATTGCTGCTGGAAAAACAGCG
AATAAGCATAATATTCCAGTATTATTAGACCCTGTTGGTGTTGCTGCCACCTCTTTTAGACGAAATGCTATTGATAAAAT
TTTAGAGGAAGTTCGACCAACTGTTATAAAAGGAAATGCTGGTGAACTAGCAAGTCTCGTTAATATCAATCTCGAAGCAA
AAGGAGTAGACTCCATTGGTGACGGAAATTTAGATGTTATTGCGCAAAAAGTAAGTGAAAAATATAACACTGCAGTTGTT
GTCACCGGAGAAACGGATGTTATTTATGTAGATAATCATTTGATTCATAATGGTACAGGTCATCATTACCTATCATCAAT
AACTGGTTCCGGTTGTTTATTAGGCTCCATTATCGCGGCGTGCTTAACCACAAAATCCACACTAAAAGATCAATTAATGA
CAGCGGTCTCGTTCTATGGACTTTCTGCTTCTTATGCAGCTAATCAAGAAAATGTTCATGGTACAGGTAGTTTTTTACCT
GTATTCATTGATTCGCTTTCTAAAATGCCACAGGAACTTACGGAAGGAGAATAA

Upstream 100 bases:

>100_bases
GCAATATCCAAATCATTTGAGCTCGGTAATGGTAATGGACCAACGAATCATTGGGCGACTCGAGAAAGGTAATGGAGATA
TTAAGGAAAGGGAGATAAAT

Downstream 100 bases:

>100_bases
ATCATTATGAAATTCGATAAACATATGCTTCGAAAGTATTTTATTATGGGTAGTCAAAACTGCCATCGAGACCCACGGGA
GATATTAAAAGAGGCAGCCT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQVDIIKQVRDTKPLVHHLTNQVVMNFSANGLLSFGGSPIMAKAIEEVEQIAAISNAVLINIGTLTSTELESMIAAGKTA
NKHNIPVLLDPVGVAATSFRRNAIDKILEEVRPTVIKGNAGELASLVNINLEAKGVDSIGDGNLDVIAQKVSEKYNTAVV
VTGETDVIYVDNHLIHNGTGHHYLSSITGSGCLLGSIIAACLTTKSTLKDQLMTAVSFYGLSASYAANQENVHGTGSFLP
VFIDSLSKMPQELTEGE

Sequences:

>Translated_257_residues
MQVDIIKQVRDTKPLVHHLTNQVVMNFSANGLLSFGGSPIMAKAIEEVEQIAAISNAVLINIGTLTSTELESMIAAGKTA
NKHNIPVLLDPVGVAATSFRRNAIDKILEEVRPTVIKGNAGELASLVNINLEAKGVDSIGDGNLDVIAQKVSEKYNTAVV
VTGETDVIYVDNHLIHNGTGHHYLSSITGSGCLLGSIIAACLTTKSTLKDQLMTAVSFYGLSASYAANQENVHGTGSFLP
VFIDSLSKMPQELTEGE
>Mature_257_residues
MQVDIIKQVRDTKPLVHHLTNQVVMNFSANGLLSFGGSPIMAKAIEEVEQIAAISNAVLINIGTLTSTELESMIAAGKTA
NKHNIPVLLDPVGVAATSFRRNAIDKILEEVRPTVIKGNAGELASLVNINLEAKGVDSIGDGNLDVIAQKVSEKYNTAVV
VTGETDVIYVDNHLIHNGTGHHYLSSITGSGCLLGSIIAACLTTKSTLKDQLMTAVSFYGLSASYAANQENVHGTGSFLP
VFIDSLSKMPQELTEGE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=263, Percent_Identity=34.6007604562738, Blast_Score=150, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6325042, Length=285, Percent_Identity=34.7368421052632, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM1_OCEIH (Q8ESZ2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691394.1
- ProteinModelPortal:   Q8ESZ2
- SMR:   Q8ESZ2
- GeneID:   1015744
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0473
- NMPDR:   fig|221109.1.peg.479
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   OIHE221109:OB0473-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27226; Mature: 27226

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 117-117 BINDING 162-162 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVDIIKQVRDTKPLVHHLTNQVVMNFSANGLLSFGGSPIMAKAIEEVEQIAAISNAVLI
CCCHHHHHHHCCHHHHHHHHHHHEEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCEEEE
NIGTLTSTELESMIAAGKTANKHNIPVLLDPVGVAATSFRRNAIDKILEEVRPTVIKGNA
EECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECCC
GELASLVNINLEAKGVDSIGDGNLDVIAQKVSEKYNTAVVVTGETDVIYVDNHLIHNGTG
HHHHHHEEECEEECCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEEECCEEEECCCC
HHYLSSITGSGCLLGSIIAACLTTKSTLKDQLMTAVSFYGLSASYAANQENVHGTGSFLP
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHH
VFIDSLSKMPQELTEGE
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVDIIKQVRDTKPLVHHLTNQVVMNFSANGLLSFGGSPIMAKAIEEVEQIAAISNAVLI
CCCHHHHHHHCCHHHHHHHHHHHEEECCCCCEEECCCCHHHHHHHHHHHHHHHHCCEEEE
NIGTLTSTELESMIAAGKTANKHNIPVLLDPVGVAATSFRRNAIDKILEEVRPTVIKGNA
EECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECCC
GELASLVNINLEAKGVDSIGDGNLDVIAQKVSEKYNTAVVVTGETDVIYVDNHLIHNGTG
HHHHHHEEECEEECCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEEECCEEEECCCC
HHYLSSITGSGCLLGSIIAACLTTKSTLKDQLMTAVSFYGLSASYAANQENVHGTGSFLP
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHH
VFIDSLSKMPQELTEGE
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376