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The map label for this gene is pbuE [H]

Identifier: 23097923

GI number: 23097923

Start: 495843

End: 497015

Strand: Reverse

Name: pbuE [H]

Synonym: OB0468

Alternate gene names: 23097923

Gene position: 497015-495843 (Counterclockwise)

Preceding gene: 23097926

Following gene: 23097921

Centisome position: 13.69

GC content: 35.29

Gene sequence:

>1173_bases
ATGGATAAACGAGTGTATTTATTAATGACCATCTCCTTTGTAGTTGGAATGGTAGAATTAATTATCAGTGGTATTTTAGA
TTTAGTAGCAAATGATCTTAATGTATCGATGGGACAGGCCGGGTTGCTTATCACCATCTTTTCATTAGTATTTGCAATCT
CGGCACCAATTTTATTATTTCTAACAGCTTCGATTGAACGAAAAAAATTAACATTAATTGCTTTAAGTACTTTTTTTATA
GGTAATTTAATAGCTATATTTAGTTTTTCCTATACAATGCTATTTATAAGTAGAATTATCTCTGCTGCAAGTGCTTCGAT
CCTTATCGTACTTTGTATCATGATGGCATCATCCATTGTTCAACCTGAATACCGCGGAAGAGCCATTGGAATTGTCAGCA
TGGGCGTCAGCGGTTCATTAGTATTTGGTATTCCGTTTGGTTTAATACTTGGAGAGGCATTCGGATGGCGAGCGCCATTC
GTACTCATAGCGATCTTAACCGTTTTATCTATGGTAGGTGTTTATTTCTTTATGACGAAGATTTATACATCCGAAAAGCC
TTTACCATTACAATCCCAGCTTAAAATGGTTAGTAGTAAATCCGTCTTATTTGCATTACTAACAACATTATTTGTTTTGG
CAGGACATACTACGTTATACGCCTACCTAAACCCTTTCGTTAGTGAAATGATGAATATGAATGGTTTTTGGCTTAGCGTT
GTTTATTTAGCCTTTGGAATCGCTGCAGTTAGTGGCGGTGGTGCGGGCGGAGCTTTATCTGATCATTTTGGACCAAAACG
CACTATTCTTACCGTAATTATACTATTCATTTTAGCAATAATCTCCTTACCTTTTGCTACTTTTAATATAATTATATTCT
TTGTTGTATTAATTATTTGGGGTATGATGAGTTGGGCGGTGTCACCACCAGTTCAGAGCTTCTTAATTGAAACTGCTCCA
AAAAATGCAGATATATTAGTGAGTCTAAATAATTCTGCATTACACTTCGGAATAGCAATTGGCTCGTTCATTGGTAGTTT
CGTCATTGAAAAGTCTCATGTACAATTCAATGCGTTTGTAGGAGGAGTAATAGTTCTTATCGGTCTGATCACATCGATTA
TTGCTTTAAAAGGATATAAAGATAAACAATCGCTAGTTTCTTCATCTAAATAA

Upstream 100 bases:

>100_bases
CCGTGTAAAAAATTTGTAAATAAATACTATTCTCTTGTACTTCTGTAAAAATAAGTCTACGATAGTCTTGTTGCGAAATT
TTATGTAAGGCTGTGTTGAC

Downstream 100 bases:

>100_bases
ATTATACTTTTAAAAAAGCAAATAAAGCTCTACACCTCAGAAATTAGGTGTAGAGCTTTTATATAACTTTCCTAAATAAT
AATATCTTCAAAGCTTGGAA

Product: chloramphenicol resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDKRVYLLMTISFVVGMVELIISGILDLVANDLNVSMGQAGLLITIFSLVFAISAPILLFLTASIERKKLTLIALSTFFI
GNLIAIFSFSYTMLFISRIISAASASILIVLCIMMASSIVQPEYRGRAIGIVSMGVSGSLVFGIPFGLILGEAFGWRAPF
VLIAILTVLSMVGVYFFMTKIYTSEKPLPLQSQLKMVSSKSVLFALLTTLFVLAGHTTLYAYLNPFVSEMMNMNGFWLSV
VYLAFGIAAVSGGGAGGALSDHFGPKRTILTVIILFILAIISLPFATFNIIIFFVVLIIWGMMSWAVSPPVQSFLIETAP
KNADILVSLNNSALHFGIAIGSFIGSFVIEKSHVQFNAFVGGVIVLIGLITSIIALKGYKDKQSLVSSSK

Sequences:

>Translated_390_residues
MDKRVYLLMTISFVVGMVELIISGILDLVANDLNVSMGQAGLLITIFSLVFAISAPILLFLTASIERKKLTLIALSTFFI
GNLIAIFSFSYTMLFISRIISAASASILIVLCIMMASSIVQPEYRGRAIGIVSMGVSGSLVFGIPFGLILGEAFGWRAPF
VLIAILTVLSMVGVYFFMTKIYTSEKPLPLQSQLKMVSSKSVLFALLTTLFVLAGHTTLYAYLNPFVSEMMNMNGFWLSV
VYLAFGIAAVSGGGAGGALSDHFGPKRTILTVIILFILAIISLPFATFNIIIFFVVLIIWGMMSWAVSPPVQSFLIETAP
KNADILVSLNNSALHFGIAIGSFIGSFVIEKSHVQFNAFVGGVIVLIGLITSIIALKGYKDKQSLVSSSK
>Mature_390_residues
MDKRVYLLMTISFVVGMVELIISGILDLVANDLNVSMGQAGLLITIFSLVFAISAPILLFLTASIERKKLTLIALSTFFI
GNLIAIFSFSYTMLFISRIISAASASILIVLCIMMASSIVQPEYRGRAIGIVSMGVSGSLVFGIPFGLILGEAFGWRAPF
VLIAILTVLSMVGVYFFMTKIYTSEKPLPLQSQLKMVSSKSVLFALLTTLFVLAGHTTLYAYLNPFVSEMMNMNGFWLSV
VYLAFGIAAVSGGGAGGALSDHFGPKRTILTVIILFILAIISLPFATFNIIIFFVVLIIWGMMSWAVSPPVQSFLIETAP
KNADILVSLNNSALHFGIAIGSFIGSFVIEKSHVQFNAFVGGVIVLIGLITSIIALKGYKDKQSLVSSSK

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=319, Percent_Identity=26.0188087774295, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786595, Length=391, Percent_Identity=25.0639386189258, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787808, Length=377, Percent_Identity=25.1989389920424, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI145693198, Length=356, Percent_Identity=26.123595505618, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42040; Mature: 42040

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRVYLLMTISFVVGMVELIISGILDLVANDLNVSMGQAGLLITIFSLVFAISAPILLF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTASIERKKLTLIALSTFFIGNLIAIFSFSYTMLFISRIISAASASILIVLCIMMASSIV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPEYRGRAIGIVSMGVSGSLVFGIPFGLILGEAFGWRAPFVLIAILTVLSMVGVYFFMTK
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IYTSEKPLPLQSQLKMVSSKSVLFALLTTLFVLAGHTTLYAYLNPFVSEMMNMNGFWLSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHH
VYLAFGIAAVSGGGAGGALSDHFGPKRTILTVIILFILAIISLPFATFNIIIFFVVLIIW
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GMMSWAVSPPVQSFLIETAPKNADILVSLNNSALHFGIAIGSFIGSFVIEKSHVQFNAFV
HHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGVIVLIGLITSIIALKGYKDKQSLVSSSK
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MDKRVYLLMTISFVVGMVELIISGILDLVANDLNVSMGQAGLLITIFSLVFAISAPILLF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTASIERKKLTLIALSTFFIGNLIAIFSFSYTMLFISRIISAASASILIVLCIMMASSIV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPEYRGRAIGIVSMGVSGSLVFGIPFGLILGEAFGWRAPFVLIAILTVLSMVGVYFFMTK
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IYTSEKPLPLQSQLKMVSSKSVLFALLTTLFVLAGHTTLYAYLNPFVSEMMNMNGFWLSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHH
VYLAFGIAAVSGGGAGGALSDHFGPKRTILTVIILFILAIISLPFATFNIIIFFVVLIIW
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GMMSWAVSPPVQSFLIETAPKNADILVSLNNSALHFGIAIGSFIGSFVIEKSHVQFNAFV
HHHHHHCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGVIVLIGLITSIIALKGYKDKQSLVSSSK
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]