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The map label for this gene is cysS

Identifier: 23097554

GI number: 23097554

Start: 119737

End: 121143

Strand: Direct

Name: cysS

Synonym: OB0099

Alternate gene names: 23097554

Gene position: 119737-121143 (Clockwise)

Preceding gene: 23097553

Following gene: 23097555

Centisome position: 3.3

GC content: 36.03

Gene sequence:

>1407_bases
ATGACCATACAAATATATAATACATTAACAAGACAAAAAGAAATATTTAAGCCACTAGAAGAAGGTAAGGTTAAAATGTA
TGTATGCGGTCCGACTGTATACAACTATATTCATATTGGTAATGCAAGACCCGCAATTGTGTTTGATACTGTTCGCCGGT
ATTTGGAATATAGAGGATTTGATGTAGATTATGTATTAAACTTCACGGACGTAGATGATAAAATCATAAAAACTGCAAAA
GACGTTGGTGAAGAAGTTCCAGTGCTTGCGGATAGATTTGTGAATGCGTATTTAGAAGACGTAGGAGCACTTGGTGTAAA
AAAAGCTACAAAGAATCCAAGAGTAATGGATACGATGGATGATATTATTGCTTTTATTTCAGCCTTAATTGAAAAAGGCT
ATGCGTATGAAGCGGATGGAGACGTATATTTTAAACCAAGATCCTTTGACACGTATGGCAAATTATCTCATCAATCTATT
GATGAACTGCGTTCTGGAGCGCGAATTCAAGTAGGAGAGAAAAAAGAAGATCCACTTGACTTTGCATTATGGAAGCAAGC
AAAAGACGATGAAATTGCTTGGAAATCTCCTTGGGGTGAAGGAAGACCAGGTTGGCATATTGAATGCTCGGCAATGGTGA
AGAAACATTTAGGAGATACGATTGATATCCACGCTGGTGGACAGGATTTAACTTTCCCACATCACGAAAATGAAATAGCA
CAATCAGAAGCAATGAACGGGGAGACTTTTGCAAACTATTGGATGCATAATGGGTATATTAATATTGATAATGAGAAAAT
GTCGAAGTCACTTGGTAACTTTGTACTTGCTAGAGACTTAATCCAAGCACATGATCCAAGAGTTTTACGCTTCTTTATGT
TATCTGTTCATTATCGAAATCCGATTAACTTCACTGAAGCTCTACTTGAAAGTGCAAAAACAAGCTTAGAGCGTATTCAA
ACAGCTTATCATAATTTATCTCATCGACGCGATTCAAGTTTAAATCTAACTAATGATGATGCTAAATGGCTGCAGTTGGT
ATCAGCAGCAATGAGCAAATTTGAACAAGATATGGACGATGATTTCAATACAGCGAATGCAATTTCGGTTTTATTTGATT
TATCGAAAGAAGCGAATGTTTACCTACAAGAAAACCAAACTTCTACTGAAGTTATAGATGCATTCCAAGATGCGATTTCA
CAGATTCTAACAGTATTAGGAATTAATATTTTAGAAGATGAAGAAACATTACTTGATGAAACCATTGAAGCGTTAATTAA
AGAAAGAAATGAAGCACGGAAAAATCGTAATTTCGCTAGAGCGGATGAGATTCGTGATATGCTCAAAGAAAAAGGTATCG
TACTCGAAGATACTCCACAAGGTGTGCGTTGGAAAAGAGGTAAGTAA

Upstream 100 bases:

>100_bases
GATCCTGTCTTTGATAAATGCGCCCAATTACAAACACAAATTGATGAGCTAAAAGCGGAAATGGCTTCGATGAAAGAGGA
AAAAAGGAGGGTCTTAGAAA

Downstream 100 bases:

>100_bases
TGAAACCGAATGATGTGAAGCAGCTAAAGAGCCTTGCACTTGCTTATATAGGTGATTCTATATTTGAATTATATATTCGA
GAATATCTATTAGAGCGTGG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTIQIYNTLTRQKEIFKPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRRYLEYRGFDVDYVLNFTDVDDKIIKTAK
DVGEEVPVLADRFVNAYLEDVGALGVKKATKNPRVMDTMDDIIAFISALIEKGYAYEADGDVYFKPRSFDTYGKLSHQSI
DELRSGARIQVGEKKEDPLDFALWKQAKDDEIAWKSPWGEGRPGWHIECSAMVKKHLGDTIDIHAGGQDLTFPHHENEIA
QSEAMNGETFANYWMHNGYINIDNEKMSKSLGNFVLARDLIQAHDPRVLRFFMLSVHYRNPINFTEALLESAKTSLERIQ
TAYHNLSHRRDSSLNLTNDDAKWLQLVSAAMSKFEQDMDDDFNTANAISVLFDLSKEANVYLQENQTSTEVIDAFQDAIS
QILTVLGINILEDEETLLDETIEALIKERNEARKNRNFARADEIRDMLKEKGIVLEDTPQGVRWKRGK

Sequences:

>Translated_468_residues
MTIQIYNTLTRQKEIFKPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRRYLEYRGFDVDYVLNFTDVDDKIIKTAK
DVGEEVPVLADRFVNAYLEDVGALGVKKATKNPRVMDTMDDIIAFISALIEKGYAYEADGDVYFKPRSFDTYGKLSHQSI
DELRSGARIQVGEKKEDPLDFALWKQAKDDEIAWKSPWGEGRPGWHIECSAMVKKHLGDTIDIHAGGQDLTFPHHENEIA
QSEAMNGETFANYWMHNGYINIDNEKMSKSLGNFVLARDLIQAHDPRVLRFFMLSVHYRNPINFTEALLESAKTSLERIQ
TAYHNLSHRRDSSLNLTNDDAKWLQLVSAAMSKFEQDMDDDFNTANAISVLFDLSKEANVYLQENQTSTEVIDAFQDAIS
QILTVLGINILEDEETLLDETIEALIKERNEARKNRNFARADEIRDMLKEKGIVLEDTPQGVRWKRGK
>Mature_467_residues
TIQIYNTLTRQKEIFKPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRRYLEYRGFDVDYVLNFTDVDDKIIKTAKD
VGEEVPVLADRFVNAYLEDVGALGVKKATKNPRVMDTMDDIIAFISALIEKGYAYEADGDVYFKPRSFDTYGKLSHQSID
ELRSGARIQVGEKKEDPLDFALWKQAKDDEIAWKSPWGEGRPGWHIECSAMVKKHLGDTIDIHAGGQDLTFPHHENEIAQ
SEAMNGETFANYWMHNGYINIDNEKMSKSLGNFVLARDLIQAHDPRVLRFFMLSVHYRNPINFTEALLESAKTSLERIQT
AYHNLSHRRDSSLNLTNDDAKWLQLVSAAMSKFEQDMDDDFNTANAISVLFDLSKEANVYLQENQTSTEVIDAFQDAISQ
ILTVLGINILEDEETLLDETIEALIKERNEARKNRNFARADEIRDMLKEKGIVLEDTPQGVRWKRGK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=480, Percent_Identity=34.1666666666667, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI303304987, Length=414, Percent_Identity=29.7101449275362, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI21269877, Length=414, Percent_Identity=29.7101449275362, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI10835051, Length=414, Percent_Identity=29.7101449275362, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI62240992, Length=414, Percent_Identity=29.7101449275362, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1786737, Length=474, Percent_Identity=45.3586497890295, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17509797, Length=354, Percent_Identity=31.0734463276836, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17509795, Length=354, Percent_Identity=31.0734463276836, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324082, Length=332, Percent_Identity=33.433734939759, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=34.0594059405941, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19922364, Length=348, Percent_Identity=32.4712643678161, Blast_Score=178, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_OCEIH (Q8ETZ9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691020.1
- ProteinModelPortal:   Q8ETZ9
- SMR:   Q8ETZ9
- GeneID:   1015179
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0099
- NMPDR:   fig|221109.1.peg.100
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   OIHE221109:OB0099-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53569; Mature: 53438

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQIYNTLTRQKEIFKPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRRYLEYRGF
CEEEEEHHHHHHHHHHCHHHCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
DVDYVLNFTDVDDKIIKTAKDVGEEVPVLADRFVNAYLEDVGALGVKKATKNPRVMDTMD
CEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHH
DIIAFISALIEKGYAYEADGDVYFKPRSFDTYGKLSHQSIDELRSGARIQVGEKKEDPLD
HHHHHHHHHHHCCCEEECCCCEEECCCCCCHHHCHHHHHHHHHHCCCEEEECCCCCCCCH
FALWKQAKDDEIAWKSPWGEGRPGWHIECSAMVKKHLGDTIDIHAGGQDLTFPHHENEIA
HHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHH
QSEAMNGETFANYWMHNGYINIDNEKMSKSLGNFVLARDLIQAHDPRVLRFFMLSVHYRN
HHHCCCCCHHHHHHEECCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
PINFTEALLESAKTSLERIQTAYHNLSHRRDSSLNLTNDDAKWLQLVSAAMSKFEQDMDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DFNTANAISVLFDLSKEANVYLQENQTSTEVIDAFQDAISQILTVLGINILEDEETLLDE
CCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHH
TIEALIKERNEARKNRNFARADEIRDMLKEKGIVLEDTPQGVRWKRGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TIQIYNTLTRQKEIFKPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRRYLEYRGF
EEEEEHHHHHHHHHHCHHHCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
DVDYVLNFTDVDDKIIKTAKDVGEEVPVLADRFVNAYLEDVGALGVKKATKNPRVMDTMD
CEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHH
DIIAFISALIEKGYAYEADGDVYFKPRSFDTYGKLSHQSIDELRSGARIQVGEKKEDPLD
HHHHHHHHHHHCCCEEECCCCEEECCCCCCHHHCHHHHHHHHHHCCCEEEECCCCCCCCH
FALWKQAKDDEIAWKSPWGEGRPGWHIECSAMVKKHLGDTIDIHAGGQDLTFPHHENEIA
HHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHH
QSEAMNGETFANYWMHNGYINIDNEKMSKSLGNFVLARDLIQAHDPRVLRFFMLSVHYRN
HHHCCCCCHHHHHHEECCEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
PINFTEALLESAKTSLERIQTAYHNLSHRRDSSLNLTNDDAKWLQLVSAAMSKFEQDMDD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DFNTANAISVLFDLSKEANVYLQENQTSTEVIDAFQDAISQILTVLGINILEDEETLLDE
CCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHH
TIEALIKERNEARKNRNFARADEIRDMLKEKGIVLEDTPQGVRWKRGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376