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The map label for this gene is glmU

Identifier: 23097513

GI number: 23097513

Start: 56254

End: 57621

Strand: Direct

Name: glmU

Synonym: OB0058

Alternate gene names: 23097513

Gene position: 56254-57621 (Clockwise)

Preceding gene: 23097512

Following gene: 23097514

Centisome position: 1.55

GC content: 38.45

Gene sequence:

>1368_bases
ATGGCGAATCGATTTGCTGTAATATTAGCAGCGGGGCAAGGGACTAGGATGAAATCAAAGCTACATAAAATGCTTCATCC
AGTTGCTGGCAGACCAATGGTGCAACATGTCGTTGATCAGCTGCAACAGGTTAACTTAAATAAAATTGTTACTATTGTTG
GATTTGGGGCAGAAAAGGTGAAGGCTCAACTTGGGTCTTCAAGTGAATTTGCATTTCAAAAGGAACAGCTGGGTACCGGC
CATGCTGTTTTACAGGCAGAGGATCTTTTGAAAGATGAAAAAGGTGTAACAGTTGTTGCTTGTGGAGATACACCTCTTAT
TCGTGCCGAAACATTTGAAGAACTGATTCAATATCATAAGAAAACAGGGGCTAAAGCAAGTATTCTGACAACCAAAGTAG
AAAACCCTACAGGATACGGACGTGTCGTTAGAAATGACCAAGACGAGGTAGAGAGAATCGTTGAACATAAAGATGCTAAT
GAAGGCGAGAGATTAATTCAAGAAATTAATACGGGGACATATTGTTTTGATAATCAAGCCTTGTTTGCAGCTTTAAAGGA
AGTTTCCAATGATAATGCACAGGGAGAATATTATTTACCTGATGTGATTAAGATATTACGTCATCAAGGTGATACGGTTA
GCGCATTTATGACTCCTGATTTTGATGAAACAATAGGGATAAATGATCGAATAGCGCTCGCAGAAGCTGAAAAAATAATG
AAAAAGCGTATTAATGAACAACATATGCGTAATGGAGTAAGTATTATTGATCCTGAGCAAACTTATATTGAACCAGATGT
ACAAATTGAATCGGACGTTATACTCCATCCTGGTACAGTTTTAAAGGGTGAAACGATTATTCGCACTGGAGCGGAAATCG
GCCCGCATTCTGAACTTAAGGATTGTGAAGTTGGGGAAGATACTGTCATTAGACATAGCGTAGCGACAAGCAGTAAAGTA
GGAAATAGAGTAAATATTGGTCCGTATGCTCATATTAGGCCAGAGTCACGTGTGGGTAACGATACAAAGGTTGGAAATTT
CGTAGAGATTAAGAAAACAAACCTAGGTGATCATAGTAAAGTATCTCATTTAAGTTATATTGGTGATGCTGACGTTGGAG
AAAGAGTCAATGTTGGCTGCGGAACCATTACAGTAAATTATGACGGTACGAATAAATACTTAACTACAATTGAAGACGAC
GCATTTATTGGTTGTAATTCGAACCTTATTGCACCTGTAACGGTTGGAAAAGGGTCTTACGTAGCTGCCGGATCTACGAT
TACAAAAAATGTACCCGAAAATGCATTGTCAATAGCAAGAGCGAGACAAACAAATAAAGAAGAATATGCATCTAAATTCA
AAAAATAG

Upstream 100 bases:

>100_bases
GTTATTCCTTTAATATATCAATGTTTTCACTTTTATTGAAAAGATGGGCCAATTACGATATATTTTTAAGTGGATATTTA
GAGATAATGGAGGTTTCCTA

Downstream 100 bases:

>100_bases
ATGAACAATTTTATGGAGGTAATCAAACAATGACATCCAGTTATAAAGATTCGTCGTTAAAAGTTTTTTCATTAAATTCA
AACCCTGAGTTAGCAAATGA

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MANRFAVILAAGQGTRMKSKLHKMLHPVAGRPMVQHVVDQLQQVNLNKIVTIVGFGAEKVKAQLGSSSEFAFQKEQLGTG
HAVLQAEDLLKDEKGVTVVACGDTPLIRAETFEELIQYHKKTGAKASILTTKVENPTGYGRVVRNDQDEVERIVEHKDAN
EGERLIQEINTGTYCFDNQALFAALKEVSNDNAQGEYYLPDVIKILRHQGDTVSAFMTPDFDETIGINDRIALAEAEKIM
KKRINEQHMRNGVSIIDPEQTYIEPDVQIESDVILHPGTVLKGETIIRTGAEIGPHSELKDCEVGEDTVIRHSVATSSKV
GNRVNIGPYAHIRPESRVGNDTKVGNFVEIKKTNLGDHSKVSHLSYIGDADVGERVNVGCGTITVNYDGTNKYLTTIEDD
AFIGCNSNLIAPVTVGKGSYVAAGSTITKNVPENALSIARARQTNKEEYASKFKK

Sequences:

>Translated_455_residues
MANRFAVILAAGQGTRMKSKLHKMLHPVAGRPMVQHVVDQLQQVNLNKIVTIVGFGAEKVKAQLGSSSEFAFQKEQLGTG
HAVLQAEDLLKDEKGVTVVACGDTPLIRAETFEELIQYHKKTGAKASILTTKVENPTGYGRVVRNDQDEVERIVEHKDAN
EGERLIQEINTGTYCFDNQALFAALKEVSNDNAQGEYYLPDVIKILRHQGDTVSAFMTPDFDETIGINDRIALAEAEKIM
KKRINEQHMRNGVSIIDPEQTYIEPDVQIESDVILHPGTVLKGETIIRTGAEIGPHSELKDCEVGEDTVIRHSVATSSKV
GNRVNIGPYAHIRPESRVGNDTKVGNFVEIKKTNLGDHSKVSHLSYIGDADVGERVNVGCGTITVNYDGTNKYLTTIEDD
AFIGCNSNLIAPVTVGKGSYVAAGSTITKNVPENALSIARARQTNKEEYASKFKK
>Mature_454_residues
ANRFAVILAAGQGTRMKSKLHKMLHPVAGRPMVQHVVDQLQQVNLNKIVTIVGFGAEKVKAQLGSSSEFAFQKEQLGTGH
AVLQAEDLLKDEKGVTVVACGDTPLIRAETFEELIQYHKKTGAKASILTTKVENPTGYGRVVRNDQDEVERIVEHKDANE
GERLIQEINTGTYCFDNQALFAALKEVSNDNAQGEYYLPDVIKILRHQGDTVSAFMTPDFDETIGINDRIALAEAEKIMK
KRINEQHMRNGVSIIDPEQTYIEPDVQIESDVILHPGTVLKGETIIRTGAEIGPHSELKDCEVGEDTVIRHSVATSSKVG
NRVNIGPYAHIRPESRVGNDTKVGNFVEIKKTNLGDHSKVSHLSYIGDADVGERVNVGCGTITVNYDGTNKYLTTIEDDA
FIGCNSNLIAPVTVGKGSYVAAGSTITKNVPENALSIARARQTNKEEYASKFKK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Homo sapiens, GI11761621, Length=369, Percent_Identity=23.8482384823848, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI11761619, Length=338, Percent_Identity=25.1479289940828, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790168, Length=456, Percent_Identity=40.5701754385965, Blast_Score=332, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI133931050, Length=376, Percent_Identity=23.6702127659574, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=371, Percent_Identity=24.2587601078167, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355443, Length=332, Percent_Identity=25.9036144578313, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644084, Length=332, Percent_Identity=25.9036144578313, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_OCEIH (Q8CXP9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690979.1
- ProteinModelPortal:   Q8CXP9
- SMR:   Q8CXP9
- GeneID:   1015119
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0058
- NMPDR:   fig|221109.1.peg.58
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- BioCyc:   OIHE221109:OB0058-MONOMER
- BRENDA:   2.3.1.157
- BRENDA:   2.7.7.23
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49838; Mature: 49707

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 363-363 BINDING 73-73 BINDING 140-140 BINDING 155-155 BINDING 170-170 BINDING 387-387 BINDING 423-423 BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRFAVILAAGQGTRMKSKLHKMLHPVAGRPMVQHVVDQLQQVNLNKIVTIVGFGAEKV
CCCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHH
KAQLGSSSEFAFQKEQLGTGHAVLQAEDLLKDEKGVTVVACGDTPLIRAETFEELIQYHK
HHHCCCCCCCEEEHHHCCCCCEEEEHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHH
KTGAKASILTTKVENPTGYGRVVRNDQDEVERIVEHKDANEGERLIQEINTGTYCFDNQA
HCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCHH
LFAALKEVSNDNAQGEYYLPDVIKILRHQGDTVSAFMTPDFDETIGINDRIALAEAEKIM
HHHHHHHHCCCCCCCCEECHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHH
KKRINEQHMRNGVSIIDPEQTYIEPDVQIESDVILHPGTVLKGETIIRTGAEIGPHSELK
HHHHHHHHHHCCCEEECCCHHEECCCCEECCCEEECCCCEECCCEEEECCCCCCCCCCCC
DCEVGEDTVIRHSVATSSKVGNRVNIGPYAHIRPESRVGNDTKVGNFVEIKKTNLGDHSK
CCCCCCCCEEHHHHHHHHHCCCEEECCCCEEECCHHHCCCCCCCCCEEEEEECCCCCCHH
VSHLSYIGDADVGERVNVGCGTITVNYDGTNKYLTTIEDDAFIGCNSNLIAPVTVGKGSY
HHHHHCCCCCCCCCEEECCEEEEEEEECCCCCEEEEECCCEEECCCCCEEEEEEECCCCE
VAAGSTITKNVPENALSIARARQTNKEEYASKFKK
EECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
ANRFAVILAAGQGTRMKSKLHKMLHPVAGRPMVQHVVDQLQQVNLNKIVTIVGFGAEKV
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHH
KAQLGSSSEFAFQKEQLGTGHAVLQAEDLLKDEKGVTVVACGDTPLIRAETFEELIQYHK
HHHCCCCCCCEEEHHHCCCCCEEEEHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHH
KTGAKASILTTKVENPTGYGRVVRNDQDEVERIVEHKDANEGERLIQEINTGTYCFDNQA
HCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCHH
LFAALKEVSNDNAQGEYYLPDVIKILRHQGDTVSAFMTPDFDETIGINDRIALAEAEKIM
HHHHHHHHCCCCCCCCEECHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHH
KKRINEQHMRNGVSIIDPEQTYIEPDVQIESDVILHPGTVLKGETIIRTGAEIGPHSELK
HHHHHHHHHHCCCEEECCCHHEECCCCEECCCEEECCCCEECCCEEEECCCCCCCCCCCC
DCEVGEDTVIRHSVATSSKVGNRVNIGPYAHIRPESRVGNDTKVGNFVEIKKTNLGDHSK
CCCCCCCCEEHHHHHHHHHCCCEEECCCCEEECCHHHCCCCCCCCCEEEEEECCCCCCHH
VSHLSYIGDADVGERVNVGCGTITVNYDGTNKYLTTIEDDAFIGCNSNLIAPVTVGKGSY
HHHHHCCCCCCCCCEEECCEEEEEEEECCCCCEEEEECCCEEECCCCCEEEEEEECCCCE
VAAGSTITKNVPENALSIARARQTNKEEYASKFKK
EECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376