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The map label for this gene is prs

Identifier: 23097514

GI number: 23097514

Start: 57651

End: 58604

Strand: Direct

Name: prs

Synonym: OB0059

Alternate gene names: 23097514

Gene position: 57651-58604 (Clockwise)

Preceding gene: 23097513

Following gene: 23097515

Centisome position: 1.59

GC content: 36.69

Gene sequence:

>954_bases
ATGACATCCAGTTATAAAGATTCGTCGTTAAAAGTTTTTTCATTAAATTCAAACCCTGAGTTAGCAAATGAAATTGCTAC
TCATATTGGAACAAACTTAGGAAAATGTACGGTTTCGAAATTCAGTGATGGTGAAGTGCAAATAAACCTTGAAGAAAGCG
TTCGTGGCTGTGATGTATATGTCGTACAATCTACTTGTGCACCTGTTAATCAGCATATTATGGAATTGTTAATTATGATT
GATGCATTAAAACGAGCATCAGCCAAATCCATTAATATCGTAATGCCATACTATGGTTATGCGCGCCAAGATCGTAAAGC
GCGTTCTAGAGAACCTATTGCAGCTAAATTAGTAGCTGATTTAATTGAAAAAGCAGGTGCAAATCGCGTGATTACATTAG
ATCTGCACGCACCACAAATTCAAGGGTTCTTTGATATACCTATTGATCACTTACAAGGTGTTCCGATTCTATCGAGTTAT
TTTGAGCAGAAAAATTTGGATGATGTCATTGTTGTATCTCCAGACCATGGTGGGGTTACCAGAGCAAGAAAAATGGCAGA
CCGCTTAAAAGCGCCTATCGGAATAATTGATAAGCGTCGTCCAAAACCAAATGTTGCGGAAATCATGAATATCATTGGAA
ATATTGAAGGAAAAACAGCGATATTAATTGATGATATTATCGATACTGCTGGAACTATTACACTTGCAGCGAACGCTCTT
ATTGAGAACGGTGCAAAAGAAGTATATGCATGCTGTACACATCCAGTTTTATCTGGACCAGCAATTGAACGTATTGATAA
CTCTAAAATCAAGGAATTAGTAATCACGGATAGCATTCCATTACCTGATGAGAAATTCAGTACAAAGATTACAGCTTTAT
CAGTAGCTCCATTAATTGGTGAGGCGATTATTCGCGTTCATGAAATGCAATCCGTAAGTATCTTATTTGATTAA

Upstream 100 bases:

>100_bases
AAAATGCATTGTCAATAGCAAGAGCGAGACAAACAAATAAAGAAGAATATGCATCTAAATTCAAAAAATAGATGAACAAT
TTTATGGAGGTAATCAAACA

Downstream 100 bases:

>100_bases
TGTTTAGTACGATCTGATTTCGGGTATTAATGATATTGACCGTTTATTAACAACCAAGATGAAGCATAAGTATTTAACAA
AAAAGAAAATATAAGTTATA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTSSYKDSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVYVVQSTCAPVNQHIMELLIMI
DALKRASAKSINIVMPYYGYARQDRKARSREPIAAKLVADLIEKAGANRVITLDLHAPQIQGFFDIPIDHLQGVPILSSY
FEQKNLDDVIVVSPDHGGVTRARKMADRLKAPIGIIDKRRPKPNVAEIMNIIGNIEGKTAILIDDIIDTAGTITLAANAL
IENGAKEVYACCTHPVLSGPAIERIDNSKIKELVITDSIPLPDEKFSTKITALSVAPLIGEAIIRVHEMQSVSILFD

Sequences:

>Translated_317_residues
MTSSYKDSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVYVVQSTCAPVNQHIMELLIMI
DALKRASAKSINIVMPYYGYARQDRKARSREPIAAKLVADLIEKAGANRVITLDLHAPQIQGFFDIPIDHLQGVPILSSY
FEQKNLDDVIVVSPDHGGVTRARKMADRLKAPIGIIDKRRPKPNVAEIMNIIGNIEGKTAILIDDIIDTAGTITLAANAL
IENGAKEVYACCTHPVLSGPAIERIDNSKIKELVITDSIPLPDEKFSTKITALSVAPLIGEAIIRVHEMQSVSILFD
>Mature_316_residues
TSSYKDSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVYVVQSTCAPVNQHIMELLIMID
ALKRASAKSINIVMPYYGYARQDRKARSREPIAAKLVADLIEKAGANRVITLDLHAPQIQGFFDIPIDHLQGVPILSSYF
EQKNLDDVIVVSPDHGGVTRARKMADRLKAPIGIIDKRRPKPNVAEIMNIIGNIEGKTAILIDDIIDTAGTITLAANALI
ENGAKEVYACCTHPVLSGPAIERIDNSKIKELVITDSIPLPDEKFSTKITALSVAPLIGEAIIRVHEMQSVSILFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=308, Percent_Identity=45.4545454545455, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=45.6591639871383, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI84875539, Length=311, Percent_Identity=45.016077170418, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=44.6945337620579, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=35.7558139534884, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=34.2028985507246, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=51.4469453376206, Blast_Score=328, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=45.016077170418, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=45.016077170418, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=45.016077170418, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=45.1298701298701, Blast_Score=281, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=33.6283185840708, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=42.9487179487179, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319403, Length=314, Percent_Identity=42.3566878980892, Blast_Score=253, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=42.3076923076923, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=40.8163265306122, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=273, Percent_Identity=32.6007326007326, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355239, Length=311, Percent_Identity=46.3022508038585, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=43.4131736526946, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=33.2394366197183, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=33.2394366197183, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=33.2394366197183, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=33.2394366197183, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=32.3529411764706, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=32.3529411764706, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=32.3529411764706, Blast_Score=189, Evalue=1e-48,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_OCEIH (Q8EU34)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690980.1
- ProteinModelPortal:   Q8EU34
- SMR:   Q8EU34
- GeneID:   1015120
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0059
- NMPDR:   fig|221109.1.peg.59
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- ProtClustDB:   PRK01259
- BioCyc:   OIHE221109:OB0059-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 34567; Mature: 34436

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSYKDSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVY
CCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHCCCCCEEE
VVQSTCAPVNQHIMELLIMIDALKRASAKSINIVMPYYGYARQDRKARSREPIAAKLVAD
EEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
LIEKAGANRVITLDLHAPQIQGFFDIPIDHLQGVPILSSYFEQKNLDDVIVVSPDHGGVT
HHHHCCCCEEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHH
RARKMADRLKAPIGIIDKRRPKPNVAEIMNIIGNIEGKTAILIDDIIDTAGTITLAANAL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHHH
IENGAKEVYACCTHPVLSGPAIERIDNSKIKELVITDSIPLPDEKFSTKITALSVAPLIG
HHCCHHHHHHHHCCCCCCCCHHHHCCCCHHHEEEEECCCCCCCHHHHCEEEEEHHHHHHH
EAIIRVHEMQSVSILFD
HHHHHHHHCCEEEEEEC
>Mature Secondary Structure 
TSSYKDSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVY
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEHHCCCCCEEE
VVQSTCAPVNQHIMELLIMIDALKRASAKSINIVMPYYGYARQDRKARSREPIAAKLVAD
EEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHH
LIEKAGANRVITLDLHAPQIQGFFDIPIDHLQGVPILSSYFEQKNLDDVIVVSPDHGGVT
HHHHCCCCEEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHH
RARKMADRLKAPIGIIDKRRPKPNVAEIMNIIGNIEGKTAILIDDIIDTAGTITLAANAL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHHH
IENGAKEVYACCTHPVLSGPAIERIDNSKIKELVITDSIPLPDEKFSTKITALSVAPLIG
HHCCHHHHHHHHCCCCCCCCHHHHCCCCHHHEEEEECCCCCCCHHHHCEEEEEHHHHHHH
EAIIRVHEMQSVSILFD
HHHHHHHHCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376