Click here to switch to the map view.

The map label for this gene is proH [H]

Identifier: 23097468

GI number: 23097468

Start: 17534

End: 18346

Strand: Direct

Name: proH [H]

Synonym: OB0013

Alternate gene names: 23097468

Gene position: 17534-18346 (Clockwise)

Preceding gene: 23097467

Following gene: 23097469

Centisome position: 0.48

GC content: 38.87

Gene sequence:

>813_bases
ATGAATCAAACGATTGCATTTATGGGAGCAGGTTCGCTTGCAGAAGCGATTATTTCTGGAATTACGAAAGCTCAAATTGT
CCCAAATGAAAATATACTCGTAACCAATAAAAGTGATATAAACCGATTAAAACGAATAGAAGAAGGCTATGACATTCAAG
GAAATACGAATAAACAAGAAGTCATTAAACAAGCAGATATTATCATTTTGGCGATGAAACCAAAAGACGCTGCAACCTCA
TTATCTTTTATGAAAGAATACATTCGTCCTGATCAAATGGTGATTTCTGTAATTGCAGGACTTACAACGGAAAGAATGGA
AGCGATTTTAGAAAAAGAGATACCAGTGATTCGAACCATGCCCAATACCTCTGCGTTAATTGGATATTCAGCTACCGCCA
TTGCAAAAGGATCCTATGTTACTGATGAGCAATTAGCAGTTGCAGAACAATTATTTCAAACTGTTGGTATGACTACAATT
ATTAAAGAAAAAGATATGCATACGGTTACGGCTATCGCTGGCAGTGGGCCGGCATTCTTTTACTATATGGTCGAAGCGAT
GGAACAAGCAGCTGTAGAAGCAGGTTTGGATGCAGAAATGGCATCTCAATTAATAGCTCAAACCGTTATCGGTGCAGGAA
AAATGTTAGAATTATCTGGAGACCCAATAACACTTCGAAAAAATATTACAAGTCCTGGTGGAACAACCGAAGCCGGTGTC
CAGCAATTAATGAGTGAAGATTTTGCATCAATTGTCCAGTCTTGTGTTCAGAGGGCACGAGAACGTTCCATGGAACTTGC
TGCCAAGTCATGA

Upstream 100 bases:

>100_bases
CTAAAATAGTAATATCTATTCGGCATATACAGCATGTATGTAACCTTTTTTCAACCTTTATAAATGACTGAAACCTATCT
TGATAAGGAGGATATTGAGT

Downstream 100 bases:

>100_bases
AGGGAGAAAAACCATGCAAACACCATCCTGTAAATATTGTGGTGCACAGTGGAGTTATGCTGAAACGTTTAAAAATATTT
TTAAACCCCGTATGATATGT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 1; P5CR 1 [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNQTIAFMGAGSLAEAIISGITKAQIVPNENILVTNKSDINRLKRIEEGYDIQGNTNKQEVIKQADIIILAMKPKDAATS
LSFMKEYIRPDQMVISVIAGLTTERMEAILEKEIPVIRTMPNTSALIGYSATAIAKGSYVTDEQLAVAEQLFQTVGMTTI
IKEKDMHTVTAIAGSGPAFFYYMVEAMEQAAVEAGLDAEMASQLIAQTVIGAGKMLELSGDPITLRKNITSPGGTTEAGV
QQLMSEDFASIVQSCVQRARERSMELAAKS

Sequences:

>Translated_270_residues
MNQTIAFMGAGSLAEAIISGITKAQIVPNENILVTNKSDINRLKRIEEGYDIQGNTNKQEVIKQADIIILAMKPKDAATS
LSFMKEYIRPDQMVISVIAGLTTERMEAILEKEIPVIRTMPNTSALIGYSATAIAKGSYVTDEQLAVAEQLFQTVGMTTI
IKEKDMHTVTAIAGSGPAFFYYMVEAMEQAAVEAGLDAEMASQLIAQTVIGAGKMLELSGDPITLRKNITSPGGTTEAGV
QQLMSEDFASIVQSCVQRARERSMELAAKS
>Mature_270_residues
MNQTIAFMGAGSLAEAIISGITKAQIVPNENILVTNKSDINRLKRIEEGYDIQGNTNKQEVIKQADIIILAMKPKDAATS
LSFMKEYIRPDQMVISVIAGLTTERMEAILEKEIPVIRTMPNTSALIGYSATAIAKGSYVTDEQLAVAEQLFQTVGMTTI
IKEKDMHTVTAIAGSGPAFFYYMVEAMEQAAVEAGLDAEMASQLIAQTVIGAGKMLELSGDPITLRKNITSPGGTTEAGV
QQLMSEDFASIVQSCVQRARERSMELAAKS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361454, Length=271, Percent_Identity=32.4723247232472, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI198041662, Length=268, Percent_Identity=28.3582089552239, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI24797095, Length=268, Percent_Identity=30.9701492537313, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI24797097, Length=268, Percent_Identity=30.9701492537313, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=32.3420074349442, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17569021, Length=261, Percent_Identity=29.5019157088123, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17540664, Length=278, Percent_Identity=29.4964028776978, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320861, Length=288, Percent_Identity=27.0833333333333, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358587, Length=268, Percent_Identity=30.9701492537313, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24648116, Length=279, Percent_Identity=31.5412186379928, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24647700, Length=172, Percent_Identity=35.4651162790698, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 29117; Mature: 29117

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTIAFMGAGSLAEAIISGITKAQIVPNENILVTNKSDINRLKRIEEGYDIQGNTNKQE
CCCEEEEECCCHHHHHHHHCCHHEEECCCCCEEEECHHHHHHHHHHHCCCCCCCCCCHHH
VIKQADIIILAMKPKDAATSLSFMKEYIRPDQMVISVIAGLTTERMEAILEKEIPVIRTM
HHHHCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEC
PNTSALIGYSATAIAKGSYVTDEQLAVAEQLFQTVGMTTIIKEKDMHTVTAIAGSGPAFF
CCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHCHHHEEECCCCCEEEEECCCCCHHH
YYMVEAMEQAAVEAGLDAEMASQLIAQTVIGAGKMLELSGDPITLRKNITSPGGTTEAGV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCHHHH
QQLMSEDFASIVQSCVQRARERSMELAAKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQTIAFMGAGSLAEAIISGITKAQIVPNENILVTNKSDINRLKRIEEGYDIQGNTNKQE
CCCEEEEECCCHHHHHHHHCCHHEEECCCCCEEEECHHHHHHHHHHHCCCCCCCCCCHHH
VIKQADIIILAMKPKDAATSLSFMKEYIRPDQMVISVIAGLTTERMEAILEKEIPVIRTM
HHHHCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEC
PNTSALIGYSATAIAKGSYVTDEQLAVAEQLFQTVGMTTIIKEKDMHTVTAIAGSGPAFF
CCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHCHHHEEECCCCCEEEEECCCCCHHH
YYMVEAMEQAAVEAGLDAEMASQLIAQTVIGAGKMLELSGDPITLRKNITSPGGTTEAGV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCHHHH
QQLMSEDFASIVQSCVQRARERSMELAAKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1849493; 2493444 [H]