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The map label for this gene is dacA [H]

Identifier: 23097466

GI number: 23097466

Start: 13774

End: 15117

Strand: Direct

Name: dacA [H]

Synonym: OB0011

Alternate gene names: 23097466

Gene position: 13774-15117 (Clockwise)

Preceding gene: 23097465

Following gene: 23097467

Centisome position: 0.38

GC content: 36.53

Gene sequence:

>1344_bases
TTGAGAAATAAAATTAACAAAGTTTTACTGTTAGCGGTAGCAAGCATCCTCGTATTTACATCCATGTTGACAGCCACTAC
AACTGTCCAAGCAAATGAATTAGACCTAGAATCTGAATCAGCAATACTCGTGGATGGTGAAACAGGAAAAGTCCTGTATG
CAAAAAATCCTGATGTTGCGTTACCTCCTGCTAGCATGACAAAAATGATGACAGAATATCTTGTTTGGGAAGCAATTGAA
AACGGCGATATCACATGGGATACAACAACAGAAATTAGTGATTATCCATATAGTATATCCGCAAATAATTCTTTTTCCGG
TGTTGGATTAAGACAACAGCAGGAATATACAGTAAAAGAATTATATGAAGCGATGGCGATTAACTCTGATAATGCAACAA
CGATCGCATTAGCAGAACTAATTGCTGGCTCTGAATCTGAATTTGTTAAAATGATGAACGAAAAAGGCGAAGAGATGGGC
TTACAAGAATTTAAGTTTGTAAACTCTACTGGACTAGATAATGAAGATTTAGGTGATAATTATCCGGAGGGAACAAACCC
GAACGATACGAATCTACTATCTGCACGTTCAGCAGCTTTATTAGGCTATCATTTAGTTAATGATTATGAAGAAGCATTAG
AAATTTCTAGCATTCCGCAAACCGATTTTGGTGGACAGACCATCAATAACTGGAACTATATGTTACCACATGAAGGAGAT
AATCTAGCATCGTATTATTATGAAGGTGTCGATGGGTTAAAGACCGGCTTTACGGATTTAGCTGGTTATAGTTTTACAGG
AACTGCGGAACGTAATGGACAACGATTAATTACGGTTGTAATGAAAACCGGAAGTGAAACAGAACGATTTGAAGAGACAG
CAAAATTATTAGACTATGGTTTCTCTAATTTTGAAAATACAGAGCTATTCTCAGCAGGCTATCAAGAAGAAGGACATGAA
ACGATCCCAGTAGCAAAAGGGAAAGAAGATCAAGTGTCGATATCACTTCAAGATAGTGTGAGTGTACCTATTAAAGCAGA
TGAAAAAGATTTATATCATTTAGAATATAACATAGATCAAGATCGATTGAATGAAGATGGTGAATTAGTTGCTCCTGTAG
AAGCGAATGAAGCAATTGGAACAGCGAAGCTTGTCTATGATGGTGAAACAGAAGATTATGGATATATTAGTGATGCCGGT
TCAAATACTGGAGAATTCACGCTTGTAACGGATGAAGCAGTGGAGAAATCAAATTGGTTTATGCTGACACTACAAGCAAT
TGGAGACTTCTTTGTAAATATATTTACAAGTGCCGTTGATTGGATTAAAGGTTTATTTAGCTAA

Upstream 100 bases:

>100_bases
TCTATTTTTTTTATTACTGCTATGTTAAAATATGATAGGTGCTAAGCAGAATACATAGTATTATGTAATATAGCGATACT
TATGATGGAGGTACAAGAAA

Downstream 100 bases:

>100_bases
ATTTCCGGTCTTGAATAAAAACTAATTAGGCAATCTTATTAACTTGTGTATAAGGTATTGCAATGTTGGTAAAAATTGAT
TATGATATACCATAATAAAC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRNKINKVLLLAVASILVFTSMLTATTTVQANELDLESESAILVDGETGKVLYAKNPDVALPPASMTKMMTEYLVWEAIE
NGDITWDTTTEISDYPYSISANNSFSGVGLRQQQEYTVKELYEAMAINSDNATTIALAELIAGSESEFVKMMNEKGEEMG
LQEFKFVNSTGLDNEDLGDNYPEGTNPNDTNLLSARSAALLGYHLVNDYEEALEISSIPQTDFGGQTINNWNYMLPHEGD
NLASYYYEGVDGLKTGFTDLAGYSFTGTAERNGQRLITVVMKTGSETERFEETAKLLDYGFSNFENTELFSAGYQEEGHE
TIPVAKGKEDQVSISLQDSVSVPIKADEKDLYHLEYNIDQDRLNEDGELVAPVEANEAIGTAKLVYDGETEDYGYISDAG
SNTGEFTLVTDEAVEKSNWFMLTLQAIGDFFVNIFTSAVDWIKGLFS

Sequences:

>Translated_447_residues
MRNKINKVLLLAVASILVFTSMLTATTTVQANELDLESESAILVDGETGKVLYAKNPDVALPPASMTKMMTEYLVWEAIE
NGDITWDTTTEISDYPYSISANNSFSGVGLRQQQEYTVKELYEAMAINSDNATTIALAELIAGSESEFVKMMNEKGEEMG
LQEFKFVNSTGLDNEDLGDNYPEGTNPNDTNLLSARSAALLGYHLVNDYEEALEISSIPQTDFGGQTINNWNYMLPHEGD
NLASYYYEGVDGLKTGFTDLAGYSFTGTAERNGQRLITVVMKTGSETERFEETAKLLDYGFSNFENTELFSAGYQEEGHE
TIPVAKGKEDQVSISLQDSVSVPIKADEKDLYHLEYNIDQDRLNEDGELVAPVEANEAIGTAKLVYDGETEDYGYISDAG
SNTGEFTLVTDEAVEKSNWFMLTLQAIGDFFVNIFTSAVDWIKGLFS
>Mature_447_residues
MRNKINKVLLLAVASILVFTSMLTATTTVQANELDLESESAILVDGETGKVLYAKNPDVALPPASMTKMMTEYLVWEAIE
NGDITWDTTTEISDYPYSISANNSFSGVGLRQQQEYTVKELYEAMAINSDNATTIALAELIAGSESEFVKMMNEKGEEMG
LQEFKFVNSTGLDNEDLGDNYPEGTNPNDTNLLSARSAALLGYHLVNDYEEALEISSIPQTDFGGQTINNWNYMLPHEGD
NLASYYYEGVDGLKTGFTDLAGYSFTGTAERNGQRLITVVMKTGSETERFEETAKLLDYGFSNFENTELFSAGYQEEGHE
TIPVAKGKEDQVSISLQDSVSVPIKADEKDLYHLEYNIDQDRLNEDGELVAPVEANEAIGTAKLVYDGETEDYGYISDAG
SNTGEFTLVTDEAVEKSNWFMLTLQAIGDFFVNIFTSAVDWIKGLFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=395, Percent_Identity=30.8860759493671, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787062, Length=386, Percent_Identity=28.2383419689119, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87082022, Length=434, Percent_Identity=27.1889400921659, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87082054, Length=265, Percent_Identity=27.1698113207547, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49342; Mature: 49342

Theoretical pI: Translated: 3.83; Mature: 3.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKINKVLLLAVASILVFTSMLTATTTVQANELDLESESAILVDGETGKVLYAKNPDVA
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCEEEECCCCCC
LPPASMTKMMTEYLVWEAIENGDITWDTTTEISDYPYSISANNSFSGVGLRQQQEYTVKE
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHH
LYEAMAINSDNATTIALAELIAGSESEFVKMMNEKGEEMGLQEFKFVNSTGLDNEDLGDN
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHCCHHHHEEECCCCCCCCCCCCC
YPEGTNPNDTNLLSARSAALLGYHLVNDYEEALEISSIPQTDFGGQTINNWNYMLPHEGD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECCCCCC
NLASYYYEGVDGLKTGFTDLAGYSFTGTAERNGQRLITVVMKTGSETERFEETAKLLDYG
CHHHHHHHCCCHHHHCHHHHCCCEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHC
FSNFENTELFSAGYQEEGHETIPVAKGKEDQVSISLQDSVSVPIKADEKDLYHLEYNIDQ
CCCCCCCHHEECCCCCCCCEECCCCCCCCCEEEEEECCCCCCCEECCCCCEEEEEECCCH
DRLNEDGELVAPVEANEAIGTAKLVYDGETEDYGYISDAGSNTGEFTLVTDEAVEKSNWF
HHCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCEEEEEHHHHCCCCEE
MLTLQAIGDFFVNIFTSAVDWIKGLFS
EEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNKINKVLLLAVASILVFTSMLTATTTVQANELDLESESAILVDGETGKVLYAKNPDVA
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCEEEECCCCCC
LPPASMTKMMTEYLVWEAIENGDITWDTTTEISDYPYSISANNSFSGVGLRQQQEYTVKE
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHH
LYEAMAINSDNATTIALAELIAGSESEFVKMMNEKGEEMGLQEFKFVNSTGLDNEDLGDN
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHCCHHHHEEECCCCCCCCCCCCC
YPEGTNPNDTNLLSARSAALLGYHLVNDYEEALEISSIPQTDFGGQTINNWNYMLPHEGD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECCCCCC
NLASYYYEGVDGLKTGFTDLAGYSFTGTAERNGQRLITVVMKTGSETERFEETAKLLDYG
CHHHHHHHCCCHHHHCHHHHCCCEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHC
FSNFENTELFSAGYQEEGHETIPVAKGKEDQVSISLQDSVSVPIKADEKDLYHLEYNIDQ
CCCCCCCHHEECCCCCCCCEECCCCCCCCCEEEEEECCCCCCCEECCCCCEEEEEECCCH
DRLNEDGELVAPVEANEAIGTAKLVYDGETEDYGYISDAGSNTGEFTLVTDEAVEKSNWF
HHCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCEEEEEHHHHCCCCEE
MLTLQAIGDFFVNIFTSAVDWIKGLFS
EEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]