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The map label for this gene is dnaA

Identifier: 23097456

GI number: 23097456

Start: 300

End: 1643

Strand: Direct

Name: dnaA

Synonym: OB0001

Alternate gene names: 23097456

Gene position: 300-1643 (Clockwise)

Preceding gene: NA

Following gene: 23097457

Centisome position: 0.01

GC content: 33.41

Gene sequence:

>1344_bases
TTGGAAAATATTGAAGAATTATGGAGCGCTACATTAAAAAAAATTGAAGAAAAATTGAGTAAACCTAGCTTTGATACGTG
GCTTAAAAATACAAAAGCAGAAGCATTAGAAAAAGATACACTAATTATTTCTGCTCCAAATGAATTCGCAAGAGATTGGT
TAGAAAATCAATACACGAACTTAATTTCTCAGATGCTACTTGAAGTTACAGGATCTGAATTAAATACAAAATTTATCATT
CCTGATTCATTAGAAGAAATAGAAGAGCAAAAGCCTATGCCAAAACCAAAGCAGTCTACAGATACAGGTGACTCACCAAA
ATCGATGCTTAACTCAAAATATACTTTTGATACATTTGTCATTGGAGCTGGTAATCGTTTCGCTCATGCTGCTTCATTAG
CGGTAGCTGAAGCACCTGCAAAAGCATATAATCCTCTATTTATTTATGGGGGAGTTGGATTAGGTAAAACACATTTAATG
CACGCTATTGGTCATTATGTACGAGACCATAATCCGAATGCTAAAGTCGTTTATTTAACATCCGAAAAATTTACAAATGA
ATTTATTAACGCAATCATGGATAATAAATCCAATCATTTTCGTAATAAATACCGAAACATTGATGTTTTATTAATTGATG
ATATTCAATTTATTGCTGGAAAAGAATCGACGCAAGAAGAGTTTTTCCATACATTTAACGCATTGCACGGTGAAAGTAAA
CAAATTATTATCTCAAGTGATCGACCACCAAAAGAAATTCCAACATTAGAAGACCGATTACGTTCTCGTTTTGAATGGGG
ATTAATTACTGATATTACTCCTCCAGACTTAGAGACACGAATTGCGATTCTTAATAAGAAAGCAAAAGCAGAAGGACTCG
ATATACCAAATGAAGTCATGCTCTATATTGCAAATCAAATTAATACAAATATCCGTGAACTAGAAGGTGCATTAATTAGA
GTGGTAGCTTATTCATCATTAGTTAATCAAGATATCGATGCTTCATTAGCAGCGGATGCGTTAAAGGATATTATTCCTAG
TAGTAAACCAAAAGAAATTACCATACCAGCTATTCAAGAAATTGTTGCAGAACGCTACCACATTCGTTTAGAAGATTTTG
CTGCAAAAAAGCGGACAAAATCTATTGCATTTCCTAGACAAATAGCGATGTATTTATCGAGAGAATTAACCGATGCATCT
TTACCAAAGATCGGAGAAGAGTTTGGCGGTCGAGATCATACAACTGTCATTCATGCTCATGAAAAAATATCAAAAATGCT
AGAACAAGATACGGAATTAGATAGAGATATCGAAGAACTAAAAGAAAAGCTTAAGTCGATATAA

Upstream 100 bases:

>100_bases
GCTTCGTACATAGATAAAGATAATAATGCCTTTCCATCTATTTTAGATTGAGAGAGGCTTTTTCTGTCATGATTAAATTA
GGAAAGGAGTGAGCACTACT

Downstream 100 bases:

>100_bases
AGATATCCCCATGTGTATACTGTGTAAAACGTGTCTCACTCATGCACAGTTTATCCACATGTGGAAAACCTTTAGTAAAG
TGTAATAATGATAGTTATCC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MENIEELWSATLKKIEEKLSKPSFDTWLKNTKAEALEKDTLIISAPNEFARDWLENQYTNLISQMLLEVTGSELNTKFII
PDSLEEIEEQKPMPKPKQSTDTGDSPKSMLNSKYTFDTFVIGAGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAIGHYVRDHNPNAKVVYLTSEKFTNEFINAIMDNKSNHFRNKYRNIDVLLIDDIQFIAGKESTQEEFFHTFNALHGESK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILNKKAKAEGLDIPNEVMLYIANQINTNIRELEGALIR
VVAYSSLVNQDIDASLAADALKDIIPSSKPKEITIPAIQEIVAERYHIRLEDFAAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGEEFGGRDHTTVIHAHEKISKMLEQDTELDRDIEELKEKLKSI

Sequences:

>Translated_447_residues
MENIEELWSATLKKIEEKLSKPSFDTWLKNTKAEALEKDTLIISAPNEFARDWLENQYTNLISQMLLEVTGSELNTKFII
PDSLEEIEEQKPMPKPKQSTDTGDSPKSMLNSKYTFDTFVIGAGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAIGHYVRDHNPNAKVVYLTSEKFTNEFINAIMDNKSNHFRNKYRNIDVLLIDDIQFIAGKESTQEEFFHTFNALHGESK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILNKKAKAEGLDIPNEVMLYIANQINTNIRELEGALIR
VVAYSSLVNQDIDASLAADALKDIIPSSKPKEITIPAIQEIVAERYHIRLEDFAAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGEEFGGRDHTTVIHAHEKISKMLEQDTELDRDIEELKEKLKSI
>Mature_447_residues
MENIEELWSATLKKIEEKLSKPSFDTWLKNTKAEALEKDTLIISAPNEFARDWLENQYTNLISQMLLEVTGSELNTKFII
PDSLEEIEEQKPMPKPKQSTDTGDSPKSMLNSKYTFDTFVIGAGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAIGHYVRDHNPNAKVVYLTSEKFTNEFINAIMDNKSNHFRNKYRNIDVLLIDDIQFIAGKESTQEEFFHTFNALHGESK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILNKKAKAEGLDIPNEVMLYIANQINTNIRELEGALIR
VVAYSSLVNQDIDASLAADALKDIIPSSKPKEITIPAIQEIVAERYHIRLEDFAAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGEEFGGRDHTTVIHAHEKISKMLEQDTELDRDIEELKEKLKSI

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=465, Percent_Identity=43.6559139784946, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI226510964, Length=170, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_OCEIH (Q8EU88)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690922.1
- ProteinModelPortal:   Q8EU88
- GeneID:   1015018
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0001
- NMPDR:   fig|221109.1.peg.1
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- ProtClustDB:   PRK00149
- BioCyc:   OIHE221109:OB0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 50693; Mature: 50693

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIEELWSATLKKIEEKLSKPSFDTWLKNTKAEALEKDTLIISAPNEFARDWLENQYTN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHCCCEEEEECCHHHHHHHHHHHHHH
LISQMLLEVTGSELNTKFIIPDSLEEIEEQKPMPKPKQSTDTGDSPKSMLNSKYTFDTFV
HHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEE
IGAGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVRDHNPNAKVVYLT
EECCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
SEKFTNEFINAIMDNKSNHFRNKYRNIDVLLIDDIQFIAGKESTQEEFFHTFNALHGESK
CHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILNKKAKAEGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCHHHCCCCCCCHHHH
LYIANQINTNIRELEGALIRVVAYSSLVNQDIDASLAADALKDIIPSSKPKEITIPAIQE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHH
IVAERYHIRLEDFAAKKRTKSIAFPRQIAMYLSRELTDASLPKIGEEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHH
EKISKMLEQDTELDRDIEELKEKLKSI
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENIEELWSATLKKIEEKLSKPSFDTWLKNTKAEALEKDTLIISAPNEFARDWLENQYTN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHCCCEEEEECCHHHHHHHHHHHHHH
LISQMLLEVTGSELNTKFIIPDSLEEIEEQKPMPKPKQSTDTGDSPKSMLNSKYTFDTFV
HHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEE
IGAGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVRDHNPNAKVVYLT
EECCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
SEKFTNEFINAIMDNKSNHFRNKYRNIDVLLIDDIQFIAGKESTQEEFFHTFNALHGESK
CHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILNKKAKAEGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCHHHCCCCCCCHHHH
LYIANQINTNIRELEGALIRVVAYSSLVNQDIDASLAADALKDIIPSSKPKEITIPAIQE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHH
IVAERYHIRLEDFAAKKRTKSIAFPRQIAMYLSRELTDASLPKIGEEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHH
EKISKMLEQDTELDRDIEELKEKLKSI
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376