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The map label for this gene is dnaN [H]

Identifier: 23097457

GI number: 23097457

Start: 1830

End: 2966

Strand: Direct

Name: dnaN [H]

Synonym: OB0002

Alternate gene names: 23097457

Gene position: 1830-2966 (Clockwise)

Preceding gene: 23097456

Following gene: 23097458

Centisome position: 0.05

GC content: 33.16

Gene sequence:

>1137_bases
ATGAGATTTACAATCCAACGAGATAAGTTAATTAATGGTGTATCAAATGTAATGAAGGCCATTTCTGCAAGAACGGTAAT
TCCAATTTTGACAGGCATGAAAATAGAAGTGAAAAATCATGGAGTTACTTTAACAGGAAGTGATTCGGATATTTCAATTG
AGTATTATATTCCAATTGAAGAAGACGGTATTGTTCATGTGGAAAATATTGAAGAAGGTACAATTATACTTCAAGCAAAA
TATTTTCCGGATATAGTTAGGAAATTACCAGAAAGTACGGTTGATATTGTAGTAGATGATCAATTGAACGTAAGAATTAC
TTCTGGAAAAGCAGAATTTAATTTAAATGGACAAAGTGCTGAAGAATATCCACAGCTACCAAAAGTTCAAACAGAAAATA
GTTTTGAATTACCGATTGATCTATTAAAAAGTATGATTAAGCAAACTGTATTTGCAGTATCCACAATGGAAACTCGACCG
ATTTTAACCGGTGTAAACTTGAAACTTGTGGATAACTCACTTTCATTTACTGCAACGGATAGTCATCGTTTAGCAAGAAG
AGAAATTCCGGTTTCTAATGCACCAATCGAAATATCACAAATTGTTGTACCTGGAAAAAGTCTCAATGAATTAAACAAAA
TTCTTGGGGATTCTGAAGAAACGGTAGAAATTAGTGTGACTAATAATCAAATTTTATTCCGTACGAAGCACTTAAACTTC
CTTTCAAGACTGCTTGATGGAAATTATCCGGAAACTTCTAGATTAATCCCTGAGCAAAGTAAGACAAAAATTCAATTAAA
AACGAAAGAATTATTAGGGACAATTGATCGTGCTTCCTTATTGGCGAAAGAAGAAAGAAATAATGTCGTAAAGTTTAATG
CACCTGGTAATTCAATGATTGAAATCTCCAGTAATTCGCCAGAAGTAGGAAACGTTGTTGAGGAAATTACAGCTGACCAA
ATGGAAGGTGAAGATGTGAAGATCTCATTTAGTTCTAAATATATGATTGATGCACTAAAAGCAATCGAATATGATGAAGT
ACAAATTGAGTTTACTGGCGCAATGAGACCATTTATTATCCGACCAGTTGGGGATGATTCTATTCTACAACTTATCTTAC
CTGTTAGAACCTATTAA

Upstream 100 bases:

>100_bases
AATGATAGTTATCCACATAATCACAGGCCCTATTATTATTATTACTGTATTTTATAAATAAAAATAATAAATATAACTTC
CAAATTAGGAGGGAACTAAA

Downstream 100 bases:

>100_bases
TTAATCTAAATAATATGGGAAGAAGCTCTTTCAACGAAAGGGCTTTTTTTCATTTAGTGTAAAGGTGAGAATAAAATTGC
ATCCTAAATTTGGAGGGAGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRFTIQRDKLINGVSNVMKAISARTVIPILTGMKIEVKNHGVTLTGSDSDISIEYYIPIEEDGIVHVENIEEGTIILQAK
YFPDIVRKLPESTVDIVVDDQLNVRITSGKAEFNLNGQSAEEYPQLPKVQTENSFELPIDLLKSMIKQTVFAVSTMETRP
ILTGVNLKLVDNSLSFTATDSHRLARREIPVSNAPIEISQIVVPGKSLNELNKILGDSEETVEISVTNNQILFRTKHLNF
LSRLLDGNYPETSRLIPEQSKTKIQLKTKELLGTIDRASLLAKEERNNVVKFNAPGNSMIEISSNSPEVGNVVEEITADQ
MEGEDVKISFSSKYMIDALKAIEYDEVQIEFTGAMRPFIIRPVGDDSILQLILPVRTY

Sequences:

>Translated_378_residues
MRFTIQRDKLINGVSNVMKAISARTVIPILTGMKIEVKNHGVTLTGSDSDISIEYYIPIEEDGIVHVENIEEGTIILQAK
YFPDIVRKLPESTVDIVVDDQLNVRITSGKAEFNLNGQSAEEYPQLPKVQTENSFELPIDLLKSMIKQTVFAVSTMETRP
ILTGVNLKLVDNSLSFTATDSHRLARREIPVSNAPIEISQIVVPGKSLNELNKILGDSEETVEISVTNNQILFRTKHLNF
LSRLLDGNYPETSRLIPEQSKTKIQLKTKELLGTIDRASLLAKEERNNVVKFNAPGNSMIEISSNSPEVGNVVEEITADQ
MEGEDVKISFSSKYMIDALKAIEYDEVQIEFTGAMRPFIIRPVGDDSILQLILPVRTY
>Mature_378_residues
MRFTIQRDKLINGVSNVMKAISARTVIPILTGMKIEVKNHGVTLTGSDSDISIEYYIPIEEDGIVHVENIEEGTIILQAK
YFPDIVRKLPESTVDIVVDDQLNVRITSGKAEFNLNGQSAEEYPQLPKVQTENSFELPIDLLKSMIKQTVFAVSTMETRP
ILTGVNLKLVDNSLSFTATDSHRLARREIPVSNAPIEISQIVVPGKSLNELNKILGDSEETVEISVTNNQILFRTKHLNF
LSRLLDGNYPETSRLIPEQSKTKIQLKTKELLGTIDRASLLAKEERNNVVKFNAPGNSMIEISSNSPEVGNVVEEITADQ
MEGEDVKISFSSKYMIDALKAIEYDEVQIEFTGAMRPFIIRPVGDDSILQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=25.7978723404255, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42232; Mature: 42232

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTIQRDKLINGVSNVMKAISARTVIPILTGMKIEVKNHGVTLTGSDSDISIEYYIPIE
CCEEECHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCEEEECCCCCEEEEEEEEEC
EDGIVHVENIEEGTIILQAKYFPDIVRKLPESTVDIVVDDQLNVRITSGKAEFNLNGQSA
CCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEECCCCH
EEYPQLPKVQTENSFELPIDLLKSMIKQTVFAVSTMETRPILTGVNLKLVDNSLSFTATD
HHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEECCEEEEEECCEEEEECC
SHRLARREIPVSNAPIEISQIVVPGKSLNELNKILGDSEETVEISVTNNQILFRTKHLNF
CHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCEEEEEEHHHHH
LSRLLDGNYPETSRLIPEQSKTKIQLKTKELLGTIDRASLLAKEERNNVVKFNAPGNSMI
HHHHHCCCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
EISSNSPEVGNVVEEITADQMEGEDVKISFSSKYMIDALKAIEYDEVQIEFTGAMRPFII
EECCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEE
RPVGDDSILQLILPVRTY
EECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRFTIQRDKLINGVSNVMKAISARTVIPILTGMKIEVKNHGVTLTGSDSDISIEYYIPIE
CCEEECHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCEEEECCCCCEEEEEEEEEC
EDGIVHVENIEEGTIILQAKYFPDIVRKLPESTVDIVVDDQLNVRITSGKAEFNLNGQSA
CCCEEEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEEEEECCCCH
EEYPQLPKVQTENSFELPIDLLKSMIKQTVFAVSTMETRPILTGVNLKLVDNSLSFTATD
HHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHEECCCCCEEECCEEEEEECCEEEEECC
SHRLARREIPVSNAPIEISQIVVPGKSLNELNKILGDSEETVEISVTNNQILFRTKHLNF
CHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCEEEEEEHHHHH
LSRLLDGNYPETSRLIPEQSKTKIQLKTKELLGTIDRASLLAKEERNNVVKFNAPGNSMI
HHHHHCCCCCCHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
EISSNSPEVGNVVEEITADQMEGEDVKISFSSKYMIDALKAIEYDEVQIEFTGAMRPFII
EECCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEE
RPVGDDSILQLILPVRTY
EECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]