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The map label for this gene is insB1 [H]

Identifier: 22127226

GI number: 22127226

Start: 3693907

End: 3694275

Strand: Reverse

Name: insB1 [H]

Synonym: y3350

Alternate gene names: 22127226

Gene position: 3694275-3693907 (Counterclockwise)

Preceding gene: 22127227

Following gene: 22127225

Centisome position: 80.3

GC content: 46.34

Gene sequence:

>369_bases
ATGTGGTCATTTGTGGGCAACAAAAAGCAGCAACGTTGGCTTTGGTATGCCTGGGAGTCTCGCCTCAAACGTATTATTGC
TCATGTTTTTGGTCGTCGGAGCAAAAAGACATTTCGCCAATTATTGGGGTTGCTGTCAGGTTTCAATATCGTCTTCTGGT
GTACCGACAACTTCAGTGCTTATGAGATGTTGCCGGATGAAAAACACATCAGGAGCAAGCTTTACACGCAGCGGATTGAG
CGTGAAAACCTGAATATCCGTAATCGGTTGAAACGACTGAACCGCAAGACTCTCGGAGACTCAAAGTCGGCAGAAATGCA
TGACAGGATCATCGGTACGTTCATTGAGCGTGAGCATTACCTAGTGTGA

Upstream 100 bases:

>100_bases
TCAATGCGGTCATGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAACCACGCTCCCACTGGATAACCTGCAAATTCAGC
TTATCTGTGAAGTCGATGAA

Downstream 100 bases:

>100_bases
TACTAATAACCAGATTGAATACATGACCGAATTTTTATTTTTATGGTGTTTGTCTGTGGGTGCGTGTCAACTATAACCTT
TGAACCGAGTGAATTGGCGG

Product: insertion sequence protein

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MWSFVGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV

Sequences:

>Translated_122_residues
MWSFVGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV
>Mature_122_residues
MWSFVGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIE
RENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=120, Percent_Identity=50.8333333333333, Blast_Score=127, Evalue=2e-31,
Organism=Escherichia coli, GI1788203, Length=120, Percent_Identity=50.8333333333333, Blast_Score=127, Evalue=2e-31,
Organism=Escherichia coli, GI1786203, Length=120, Percent_Identity=50.8333333333333, Blast_Score=127, Evalue=2e-31,
Organism=Escherichia coli, GI1787223, Length=120, Percent_Identity=50, Blast_Score=124, Evalue=2e-30,
Organism=Escherichia coli, GI1786470, Length=120, Percent_Identity=50, Blast_Score=123, Evalue=3e-30,
Organism=Escherichia coli, GI1786459, Length=120, Percent_Identity=50, Blast_Score=123, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14919; Mature: 14919

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSFVGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHY
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
LV
CC
>Mature Secondary Structure
MWSFVGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YEMLPDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHY
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]