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The map label for this gene is insA [H]

Identifier: 22127227

GI number: 22127227

Start: 3694329

End: 3694607

Strand: Reverse

Name: insA [H]

Synonym: y3351

Alternate gene names: 22127227

Gene position: 3694607-3694329 (Counterclockwise)

Preceding gene: 22127228

Following gene: 22127226

Centisome position: 80.3

GC content: 51.25

Gene sequence:

>279_bases
TTGATGGCTAAGGTTGATGTGAAATGTCCATTTTGCGAGCAGTTCCACCCTGTTAAAAAGCACGGGCCAGGACGCACAGG
GCATCAGCGCTACCGCTGTCAGGCCTGCCGACGGAGCTTTCAACTAGAGTACGAGTATCGTGCCTGCCACCCCGGTATGA
AAGAGCAAATTGTCGATCTTGCTATGAACAATGCCGGTATTCGTGATACTGCACGGGCGTTGCATATCAGTATCAATGCG
GTCATGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAA

Upstream 100 bases:

>100_bases
CAAAATAAGGGCCTCCTTAAGTTGTATAACTTCAAAAAAACTGGTCATGCATTCAATGCGGAGATCATCAGGTAAAATCC
TCGCATTTATCTGAGAGGGT

Downstream 100 bases:

>100_bases
CCACGCTCCCACTGGATAACCTGCAAATTCAGCTTATCTGTGAAGTCGATGAAATGTGGTCATTTGTGGGCAACAAAAAG
CAGCAACGTTGGCTTTGGTA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MMAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDLAMNNAGIRDTARALHISINA
VMRTLKNSRRSV

Sequences:

>Translated_92_residues
MMAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDLAMNNAGIRDTARALHISINA
VMRTLKNSRRSV
>Mature_92_residues
MMAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDLAMNNAGIRDTARALHISINA
VMRTLKNSRRSV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=90, Percent_Identity=44.4444444444444, Blast_Score=95, Evalue=1e-21,
Organism=Escherichia coli, GI1788204, Length=90, Percent_Identity=44.4444444444444, Blast_Score=95, Evalue=1e-21,
Organism=Escherichia coli, GI1786204, Length=90, Percent_Identity=44.4444444444444, Blast_Score=95, Evalue=1e-21,
Organism=Escherichia coli, GI1790747, Length=90, Percent_Identity=43.3333333333333, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI87081814, Length=90, Percent_Identity=44.4444444444444, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1786471, Length=90, Percent_Identity=44.4444444444444, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1786460, Length=90, Percent_Identity=44.4444444444444, Blast_Score=94, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10647; Mature: 10647

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
10.9 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
10.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDL
CCCCCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AMNNAGIRDTARALHISINAVMRTLKNSRRSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMAKVDVKCPFCEQFHPVKKHGPGRTGHQRYRCQACRRSFQLEYEYRACHPGMKEQIVDL
CCCCCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AMNNAGIRDTARALHISINAVMRTLKNSRRSV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]