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The map label for this gene is mutY [H]

Identifier: 22127215

GI number: 22127215

Start: 3678521

End: 3679768

Strand: Direct

Name: mutY [H]

Synonym: y3339

Alternate gene names: 22127215

Gene position: 3678521-3679768 (Clockwise)

Preceding gene: 22127208

Following gene: 22127216

Centisome position: 79.95

GC content: 50.32

Gene sequence:

>1248_bases
GTGAGCGCCGCTAACGCCGCTGCGGCGTCAAGGACGAAGAGTATAAGGAAAGTTCCGGTTTACAGCACAAGAACTCTATG
CTGCAATCCAGCTCTCATTTATTTACCTGTCGGATATCGATACCTGCTTATGATGCAAGCGCAACAATTCGCGCACGTGG
TACTTGATTGGTACCAACACTTTGGCCGCAAAACCCTGCCATGGCAGTTGGATAAGACCCCCTATCAAGTATGGCTGTCA
GAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTCATCCCCTATTTTCAACGTTTTATGCTGCGCTTCCCTGATATTCA
GGCACTGGCGGCTGCGCCGTTGGATGATGTACTGCATTTATGGACCGGTTTGGGTTACTACGCCCGTGCCAGAAACCTGC
ATAAAGCGGCCCAAATGGTCGTGGAACACCATCAAGGGGAGTTTCCCACAACATTTGACCAGATACTGGCATTGCCGGGT
ATCGGGCGCTCAACTGCCGGGGCTATTTTATCGCTGTCTTTAGGCCAGCATTTTCCTATTTTGGATGGCAACGTCAAACG
GGTGCTGGCCCGTTGCTATGCCGTTGACGGCTGGCCGGGAAAAAAAGAGGTCGAAGGCCGCCTGTGGCAAATCAGCGAAG
ATGTCACACCCGCCAACGGGGTGGGCCAGTTTAATCAGGCAATGATGGATTTAGGCGCGATGGTGTGTACTCGCTCTAAA
CCTAAATGTGAACTTTGCCCATTGAATATCGGCTGTATGGCGTACGCTAACCACAGTTGGGCGCGCTATCCGGGCAAAAA
ACCTAAACAGACGTTGCCGGAAAAAACCGCCTGGTTCTTATTAATGCAAAATGGATCGCAAGTGTGGCTCGAACAGCGCC
CCCCAGTCGGCTTATGGGGCGGCTTATTCTGTTTCCCACAATTTGCTGAACAAGAAGAACTCATTCACTGGCTGCAAAAA
CAGGGTATTCCCGCCAATGAAACCCAGCAGTTAACCGCGTTTCGCCATACGTTTAGTCATTTCCATCTGGATATAGTCCC
TATATGGCTAAATACGGCCTCAGTCCGAGGATGCATGGATGATGGCGCAGGTCTCTGGTATAACTTAGCCCAGCCACCTT
CGGTAGGGTTAGCTGCTCCGGTTGAGCGTTTATTGCATCAGTTATTAAAAGATCCGTTGGCAAAAGATGAGTTAACGCAA
CAACAACTCACAAAGCAATCGCCTACCCAACCAGCTTTATTTGACTAG

Upstream 100 bases:

>100_bases
TTATATAGACTTAAAATGATTGGTGTTGCAGTAAAGAAACAAGTGAATCCCGATGAGCTTACTCATTTTAAAATCAACGA
CCAGTAAGTGATTCGGGTAA

Downstream 100 bases:

>100_bases
ATACCCAAAGAAATTGGAGATGCAGGTAGGCAGCAAGCGAATGGCAAATCGGCCTGTGCCAATTTGAACTGCATTCATGC
TAGCCCACAGGGGGAGTGAA

Product: adenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSAANAAAASRTKSIRKVPVYSTRTLCCNPALIYLPVGYRYLLMMQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLS
EVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDDVLHLWTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPG
IGRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANGVGQFNQAMMDLGAMVCTRSK
PKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQNGSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQK
QGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQ
QQLTKQSPTQPALFD

Sequences:

>Translated_415_residues
MSAANAAAASRTKSIRKVPVYSTRTLCCNPALIYLPVGYRYLLMMQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLS
EVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDDVLHLWTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPG
IGRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANGVGQFNQAMMDLGAMVCTRSK
PKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQNGSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQK
QGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQ
QQLTKQSPTQPALFD
>Mature_414_residues
SAANAAAASRTKSIRKVPVYSTRTLCCNPALIYLPVGYRYLLMMQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSE
VMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDDVLHLWTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGI
GRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANGVGQFNQAMMDLGAMVCTRSKP
KCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQNGSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQ
GIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQ
QLTKQSPTQPALFD

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=357, Percent_Identity=35.0140056022409, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI190358497, Length=357, Percent_Identity=34.733893557423, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI115298648, Length=357, Percent_Identity=35.0140056022409, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI115298650, Length=357, Percent_Identity=34.733893557423, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI115298654, Length=357, Percent_Identity=35.0140056022409, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI115298652, Length=357, Percent_Identity=35.0140056022409, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1789331, Length=344, Percent_Identity=75.8720930232558, Blast_Score=557, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 46793; Mature: 46661

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAANAAAASRTKSIRKVPVYSTRTLCCNPALIYLPVGYRYLLMMQAQQFAHVVLDWYQH
CCCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDDVLHL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHH
WTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRSTAGAILSLSLGQHFPI
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHEEEEECCCCCCC
LDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANGVGQFNQAMMDLGAMVCTRSK
CCCHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQNGSQVWLEQRPPVGLWG
CCCEECCCCCCEEEECCCCHHHCCCCCCHHHCCCCCEEEEEEECCCEEEEECCCCCCCCH
GLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMD
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEEEECCHHHHCCCC
DGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLTKQSPTQPALFD
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAANAAAASRTKSIRKVPVYSTRTLCCNPALIYLPVGYRYLLMMQAQQFAHVVLDWYQH
CCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
FGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDDVLHL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHH
WTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRSTAGAILSLSLGQHFPI
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHEEEEECCCCCCC
LDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANGVGQFNQAMMDLGAMVCTRSK
CCCHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQNGSQVWLEQRPPVGLWG
CCCEECCCCCCEEEECCCCHHHCCCCCCHHHCCCCCEEEEEEECCCEEEEECCCCCCCCH
GLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMD
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEEEECCHHHHCCCC
DGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLTKQSPTQPALFD
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]