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The map label for this gene is ldhA [H]

Identifier: 22125896

GI number: 22125896

Start: 2205964

End: 2207013

Strand: Reverse

Name: ldhA [H]

Synonym: y2004

Alternate gene names: 22125896

Gene position: 2207013-2205964 (Counterclockwise)

Preceding gene: 22125900

Following gene: 22125895

Centisome position: 47.97

GC content: 44.95

Gene sequence:

>1050_bases
TTGTATGACTTATTGAAATATGTTAAATTTATCACAATATTTTGGCGGAGAAATACGATGAAATTAGCCGTTTACAGTAC
CAAGCAGTATGACCGTAAATACCTTGAGCTGGTCAACAAAGATTTTGGCTTTGAATTGGAGTTTTTCGATTTTCTGCTTA
CCCCCAAAACCGCCAAAATGGCTGAGGGCTGTCAGGCGGTATGTCTCTTCGTCAATGATGATGGCTGCCGCGAAATATTA
ACTGAACTGGCGACTATCGGCGTTAAAATTCTGGCATTGCGTTGTGCTGGCTTTAATAACGTGGATCTGGAAGCGGCCAA
AGAGTTGGGTATACAAGTGGTCCGAGTGCCCGCATATTCACCTGAAGCTGTCGCGGAACATACCGTTGGCATGATGCTGA
GCCTTAACCGACGTATTCACCGTGCTTATCAGCGAACCCGTGATGCCAATTTCTCGCTGGAAGGTTTGATTGGTTTCAAT
ATGCATAACCGTACTGCGGGGATTATCGGCACAGGTAAAATTGGTGTTGCCACCATGCGTATCCTTAAAGGTTTTGGTAT
GCGCCTGCTGGCGTTTGACCCTTATCCAAGTGAGCAAGCTCTAGAGCTAGGTGCAGAATATGTTGATCTGAAGACCTTGT
ACGCCGAGTCTGATGTTATCTCACTGCATTGCCCGATGACGCCAGAAAACCATCATTTGCTCAATAAGCAGTCATTTGAT
CAAATGAAAGATGGCGTGATGATCATTAATACCAGCCGTGGCGGTTTAATTGATTCAACGGCGGCGATCGATGCGTTAAA
ACAACAGAAAATAGGCTCCCTCGGCATGGACGTTTATGAAAATGAACGTGATTTATTCTTTGAAGATAAATCCAATGACG
TGATTCAGGATGATGTTTTCCGCCGTTTGTCCTCTTGCCATAATGTGTTATTTACTGGGCATCAGGCGTTCCTAACCGAG
GAAGCACTCACCAGTATCTCTGTCACCACCTTGCAGAATATTGCTCAGTTGGATAAAGGCGAACCCTGCCCAAATATCAT
CACCCTCTGA

Upstream 100 bases:

>100_bases
ATAACTAGGTAACAGCTGTGATTGAAAATGGAGATAAATACCTTATCCTTTTAGTCTTAATAAGTTACACAGACTGCGAT
AAACTTATCATTGCTGAAAA

Downstream 100 bases:

>100_bases
TGCATTAACACCCAATAGCGGATAGCGAGTGTACATTCTCCCTATCCGTTATGATGAGTAGGAGATAATATGAGAAAGTT
CGTTCCAGTGGCTATTATCG

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MYDLLKYVKFITIFWRRNTMKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREIL
TELATIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFN
MHNRTAGIIGTGKIGVATMRILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFD
QMKDGVMIINTSRGGLIDSTAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTE
EALTSISVTTLQNIAQLDKGEPCPNIITL

Sequences:

>Translated_349_residues
MYDLLKYVKFITIFWRRNTMKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREIL
TELATIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFN
MHNRTAGIIGTGKIGVATMRILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFD
QMKDGVMIINTSRGGLIDSTAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTE
EALTSISVTTLQNIAQLDKGEPCPNIITL
>Mature_349_residues
MYDLLKYVKFITIFWRRNTMKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREIL
TELATIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFN
MHNRTAGIIGTGKIGVATMRILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFD
QMKDGVMIINTSRGGLIDSTAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTE
EALTSISVTTLQNIAQLDKGEPCPNIITL

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=245, Percent_Identity=34.2857142857143, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI4557497, Length=245, Percent_Identity=34.2857142857143, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI23308577, Length=199, Percent_Identity=33.6683417085427, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI145580578, Length=237, Percent_Identity=33.7552742616034, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557499, Length=237, Percent_Identity=33.7552742616034, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580575, Length=249, Percent_Identity=33.7349397590361, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=27.9863481228669, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=81.2883435582822, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=34.0314136125654, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789279, Length=236, Percent_Identity=30.9322033898305, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=254, Percent_Identity=27.5590551181102, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=261, Percent_Identity=30.6513409961686, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=195, Percent_Identity=35.8974358974359, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=234, Percent_Identity=32.4786324786325, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=234, Percent_Identity=32.0512820512821, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=222, Percent_Identity=27.9279279279279, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=126, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324980, Length=149, Percent_Identity=27.5167785234899, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646446, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646448, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646452, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646450, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472511, Length=248, Percent_Identity=33.4677419354839, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921140, Length=256, Percent_Identity=26.5625, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574286, Length=294, Percent_Identity=28.9115646258503, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552429, Length=262, Percent_Identity=28.2442748091603, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585514, Length=262, Percent_Identity=28.2442748091603, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28574282, Length=262, Percent_Identity=28.2442748091603, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28574284, Length=262, Percent_Identity=28.2442748091603, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45551003, Length=262, Percent_Identity=28.2442748091603, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571528, Length=283, Percent_Identity=27.5618374558304, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 39277; Mature: 39277

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDLLKYVKFITIFWRRNTMKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKM
CHHHHHHHHHHHHEEECCEEEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHH
AEGCQAVCLFVNDDGCREILTELATIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYS
HCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCEEEEEECCCC
PEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATMR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEECEEECCCHHHHHHH
ILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFD
HHHHCCCEEEEECCCCCHHHHHHCCHHEEHHHEEECCCEEEEECCCCCCCCCCCCCCHHH
QMKDGVMIINTSRGGLIDSTAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVF
HHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCEEEECCCCCCHHHHHH
RRLSSCHNVLFTGHQAFLTEEALTSISVTTLQNIAQLDKGEPCPNIITL
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MYDLLKYVKFITIFWRRNTMKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKM
CHHHHHHHHHHHHEEECCEEEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHH
AEGCQAVCLFVNDDGCREILTELATIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYS
HCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCEEEEEECCCC
PEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATMR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEECEEECCCHHHHHHH
ILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFD
HHHHCCCEEEEECCCCCHHHHHHCCHHEEHHHEEECCCEEEEECCCCCCCCCCCCCCHHH
QMKDGVMIINTSRGGLIDSTAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVF
HHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCEEEECCCCCCHHHHHH
RRLSSCHNVLFTGHQAFLTEEALTSISVTTLQNIAQLDKGEPCPNIITL
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]