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The map label for this gene is gloA [H]

Identifier: 22125849

GI number: 22125849

Start: 2157416

End: 2157862

Strand: Reverse

Name: gloA [H]

Synonym: y1957

Alternate gene names: 22125849

Gene position: 2157862-2157416 (Counterclockwise)

Preceding gene: 22125850

Following gene: 22125847

Centisome position: 46.9

GC content: 48.32

Gene sequence:

>447_bases
TTGCCAGAGCGTCGGGCAAAATGTAAACAGAGGGAAATTATGCGCTTACTCCATACCATGCTCCGCGTCGGTGACCTGCA
ACGTTCTATCGATTTCTACACCAAGGTATTAGGGATGCGTTTACTGCGTACCAGCGAAAATACTGAATATAAATACTCGT
TGGCATTCGTAGGCTATAGCGATGAAAGTAAAGGTTCGGTGATTGAACTGACGTATAACTGGGGCGTTGACCAGTACGAT
ATGGGCACCGCATTCGGCCATCTGGCTCTGGGTGTTGATGATGTCGCCGCAACGTGTGATCAAATTCGCCAGGCAGGCGG
TAAAGTCACCCGCGAAGCTGGCCCGGTAAAAGGCGGTAATACCATTATTGCTTTTGTTGAAGATCCAGATGGCTACAAAA
TTGAGTTAATTGAGAATAAGAGCGCGGGTGACTGCCTCGGAAACTGA

Upstream 100 bases:

>100_bases
TGGGAGGAAACCCAAAATGTAAAGTGACCTATTCATTCTCTGTAACCCATTGAACTAATTTAATTACCGGGGCTATACTT
GAACCTAATTTTATTCACTC

Downstream 100 bases:

>100_bases
TTAACTTAAGGGCACCTCGGACGGTGCCTTTTACTTATCACAATTAACCCCCTGCATCCCTCGCGAAAATTTGACATAAT
GCACGCTGAATTCGATAAAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MPERRAKCKQREIMRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYD
MGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN

Sequences:

>Translated_148_residues
MPERRAKCKQREIMRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYD
MGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN
>Mature_147_residues
PERRAKCKQREIMRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYSDESKGSVIELTYNWGVDQYDM
GTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGNTIIAFVEDPDGYKIELIENKSAGDCLGN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=36.1842105263158, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=79.2592592592593, Blast_Score=227, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323639, Length=141, Percent_Identity=39.0070921985816, Blast_Score=99, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=35.1351351351351, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=33.0935251798561, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16461; Mature: 16330

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERRAKCKQREIMRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
DESKGSVIELTYNWGVDQYDMGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGN
CCCCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TIIAFVEDPDGYKIELIENKSAGDCLGN
EEEEEEECCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PERRAKCKQREIMRLLHTMLRVGDLQRSIDFYTKVLGMRLLRTSENTEYKYSLAFVGYS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
DESKGSVIELTYNWGVDQYDMGTAFGHLALGVDDVAATCDQIRQAGGKVTREAGPVKGGN
CCCCCCEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TIIAFVEDPDGYKIELIENKSAGDCLGN
EEEEEEECCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]