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The map label for this gene is nemA [H]

Identifier: 22125850

GI number: 22125850

Start: 2157941

End: 2158798

Strand: Reverse

Name: nemA [H]

Synonym: y1958

Alternate gene names: 22125850

Gene position: 2158798-2157941 (Counterclockwise)

Preceding gene: 22125851

Following gene: 22125849

Centisome position: 46.92

GC content: 51.52

Gene sequence:

>858_bases
ATGAAAACTGCTAAATTATTCTCCCCATTAAAGGTTGGCGCGCTCACGTTGCCAAACCGTGTCTTTATGGCCCCACTCAC
CCGCTTGCGTAGCATTGAGCCTGGTGATATCCCAACGCCGTTAATGGCCGAATATTATCGCCAACGGGCCAGTGCAGGCT
TGATTATCACGGAAGCCACTCAGATTTCTTTTCAGGCGAAAGGCTATGCTGGCGCTCCAGGGCTACATACTCAAGAACAG
TTGAACGCATGGAAAAAAGTGACTCAGGGTGTACATAATGAGGGTGGCCATATTGCCGTACAACTTTGGCACGTAGGCCG
TATTTCCCACAACAGTTTACAACCAGGCCAGCAAGCCCCTGTAGCACCATCGGCGATTGCCGCAGATACCCGTACCACCG
TGCGCGATGAAACAGGTGCTTGGGTACGTGTCCCTTGCTCTACCCCACGGGCGCTGGAGACAGAAGAGATTCCGGGCATC
ATTAATGATTTCCGTCAGGCAACCGCCAATGCCCGCGAAGCAGGATTTGACTACATTGAACTGCACGCAGCCCATGGTTA
TCTGTTGCATCAATTCATGTCCCCAGCTTCCAATCAGCGTACTGACCAATATGGCGGCAGTATTGAAAACCGTACCCGTC
TGACGTTGGAAGTGGTCGATGCCACCGCCGCCGAATGGGGGGCTGACCGAATTGGTATTCGTATTTCGCCATTAGGCCCA
TTTAATGGCCTCGATAATGGTGAAGACCAAGAAGAAGCCGCACTGTATCTTATAGGACCTAATGCGGAGAGTGGGGCTAC
CGATATGACGAGCTTCATTTGTCGTTATTGTGAGCCTGGGAGGAAACCCAAAATGTAA

Upstream 100 bases:

>100_bases
ACAACCAGCCTAATAATCTACCGATCCTGACACTGACTTTTTATGTTTTTCAGGATTTACCCAGCAACTAGTTGACTGGT
CTATTAATACAGGACGAACC

Downstream 100 bases:

>100_bases
AGTGACCTATTCATTCTCTGTAACCCATTGAACTAATTTAATTACCGGGGCTATACTTGAACCTAATTTTATTCACTCTT
GCCAGAGCGTCGGGCAAAAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIGPNAESGATDMTSFICRYCEPGRKPKM

Sequences:

>Translated_285_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIGPNAESGATDMTSFICRYCEPGRKPKM
>Mature_285_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIGPNAESGATDMTSFICRYCEPGRKPKM

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=258, Percent_Identity=76.3565891472868, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1789463, Length=242, Percent_Identity=35.5371900826446, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=222, Percent_Identity=34.6846846846847, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=226, Percent_Identity=33.6283185840708, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=239, Percent_Identity=30.9623430962343, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=223, Percent_Identity=31.8385650224215, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=225, Percent_Identity=32.8888888888889, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=221, Percent_Identity=32.579185520362, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=236, Percent_Identity=27.5423728813559, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=250, Percent_Identity=40.4, Blast_Score=178, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6321973, Length=254, Percent_Identity=39.3700787401575, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 31075; Mature: 31075

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIGPNAESGATDMTSFICRYCEPGRKPKM
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIGPNAESGATDMTSFICRYCEPGRKPKM
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]