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The map label for this gene is potH [H]

Identifier: 22125290

GI number: 22125290

Start: 1546543

End: 1547889

Strand: Direct

Name: potH [H]

Synonym: y1392

Alternate gene names: 22125290

Gene position: 1546543-1547889 (Clockwise)

Preceding gene: 22125289

Following gene: 22125291

Centisome position: 33.61

GC content: 52.49

Gene sequence:

>1347_bases
TTGAGCAACGCTTCAACTCTTGGGCAGCTAAATAACGGCGATAGCTGCTGGCGTAGACCCACGGTTTCCGGAGGGAACGC
AATGAGCCAAACCGAGATGGTCACCAAACCTGTAGCGGATGAGCGGCAGCAATCTGGCCCGATACTGCGTCAACAACTGC
GCAAAGTGAAGGCGGCCTACACACGCCGATCTCTCTTACTGATTACACCGCTGCTGCTGTTTATCGTCGTTAGCTTTATT
TTCCCCATTGCGACTATCTTGGGGAAAAGTATCGATAACCCCGAATTGAGGGTGGCCATGCCGGTAACAGTGGCTGTACT
GGCGAATTGGCCTGGCGATGCGCTACCGGACGAGGCAATGTTCGAGGCATTGATCACTGATCTGCGTAACGCGCGTAGCA
ATGGATTAATGCTCTCGATCGCCAAACGGATGAGTTATGAAGATAACCGTTATCGTAGCCTGATCATGTCGACACTACGG
AAAGTACCGGCTGAAGGGCCGGTACGTGAAGCGCTAATCAGTGCGTTACCGTTGTGGGGGGAATTAAGCACTTGGCAATC
GCTGCAACGGGCGACTCAGCCCTTGACCCGCTATTACCTGCTATCTGCATTTGACCGTAAAGTAGACGTAGAAAGTGGGC
AAATTAAGGCATTACCGGCTGATCAAGCGTTGTACGTTAACGTCTTGCTGCGGACGTTATGGATGGCGGCTGTCGTCACT
CTGTTATGCGTTACCTTGGGATACCCGCTGGCATATTGGTTGGCAAAACAGCCCACTGGCCGCGCCAACCTGTTGTTGAT
GCTGGTGTTGTTACCGTTCTGGACCTCATTGATTGTGCGTACCGCCAGTTGGATTGTGCTGTTGCAGTCCAGTGGGTTAA
TCAACCAGTCGCTGCTGGGGAGTAGGCTTATCGAACAACCATTGGCGCTGGTGTTTAACCGCGCCGGGGTCTACATCTCA
ATGACCCATATCCTGCTGCCCTTTATCGTGTTGCCGCTGTATGCCGTGATGAAAGGTATTTCGCCCAATTATGTCCGAGC
CGCCATTTCATTAGGTGCGCATCCGTTTGCTGCTTTCTGGCGTGTTTATGTACCGCAAACCTACAACGGCGTTACGGCGG
GCGCGTTACTGGTCTTTATGATGGCGATTGGCTACTACATCACACCCGCATTACTCGGCGGTCCGGGTGATCAGATGGTG
AGCTACTTTGTCGCTTTCTTTACCAATACCACCATGAACTGGGGAATGGCCGCGGCGTTGGGGAGCCAGTTATTGATTAT
CGTCCTGTTGCTGTACGTGGTTTATATCCGAGTAACCCGTACCCAGGCTGAAACCGCGATTCATTAG

Upstream 100 bases:

>100_bases
ATTGATCCCGAAATCGCCGCTAATTTACCCACTGCGCCAGCCAACCTGGCTGAGGCTTTTCCCATGAACAATCAATTCTG
GGTCGAGCATAGCGAAGATC

Downstream 100 bases:

>100_bases
TTCATCTATTGATGTGTTTATCCGTTCATGAGCTTATCAACACATTGTGCCGGAGGAGACGATGCCGAAACCACAAAAGG
CACTGAAACAAAAAGGGGCG

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSNASTLGQLNNGDSCWRRPTVSGGNAMSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFI
FPIATILGKSIDNPELRVAMPVTVAVLANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLR
KVPAEGPVREALISALPLWGELSTWQSLQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVT
LLCVTLGYPLAYWLAKQPTGRANLLLMLVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSRLIEQPLALVFNRAGVYIS
MTHILLPFIVLPLYAVMKGISPNYVRAAISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMV
SYFVAFFTNTTMNWGMAAALGSQLLIIVLLLYVVYIRVTRTQAETAIH

Sequences:

>Translated_448_residues
MSNASTLGQLNNGDSCWRRPTVSGGNAMSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFI
FPIATILGKSIDNPELRVAMPVTVAVLANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLR
KVPAEGPVREALISALPLWGELSTWQSLQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVT
LLCVTLGYPLAYWLAKQPTGRANLLLMLVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSRLIEQPLALVFNRAGVYIS
MTHILLPFIVLPLYAVMKGISPNYVRAAISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMV
SYFVAFFTNTTMNWGMAAALGSQLLIIVLLLYVVYIRVTRTQAETAIH
>Mature_447_residues
SNASTLGQLNNGDSCWRRPTVSGGNAMSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIF
PIATILGKSIDNPELRVAMPVTVAVLANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRK
VPAEGPVREALISALPLWGELSTWQSLQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVTL
LCVTLGYPLAYWLAKQPTGRANLLLMLVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSRLIEQPLALVFNRAGVYISM
THILLPFIVLPLYAVMKGISPNYVRAAISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVS
YFVAFFTNTTMNWGMAAALGSQLLIIVLLLYVVYIRVTRTQAETAIH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=263, Percent_Identity=34.9809885931559, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=35.9447004608295, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787713, Length=262, Percent_Identity=29.3893129770992, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 49558; Mature: 49427

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNASTLGQLNNGDSCWRRPTVSGGNAMSQTEMVTKPVADERQQSGPILRQQLRKVKAAY
CCCCHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
TRRSLLLITPLLLFIVVSFIFPIATILGKSIDNPELRVAMPVTVAVLANWPGDALPDEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHEEEEECCCCCCCCHHHH
FEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRKVPAEGPVREALISALPLWG
HHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
ELSTWQSLQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LLCVTLGYPLAYWLAKQPTGRANLLLMLVLLPFWTSLIVRTASWIVLLQSSGLINQSLLG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
SRLIEQPLALVFNRAGVYISMTHILLPFIVLPLYAVMKGISPNYVRAAISLGAHPFAAFW
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
RVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVSYFVAFFTNTTMNWGMAAAL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
GSQLLIIVLLLYVVYIRVTRTQAETAIH
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNASTLGQLNNGDSCWRRPTVSGGNAMSQTEMVTKPVADERQQSGPILRQQLRKVKAAY
CCCHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
TRRSLLLITPLLLFIVVSFIFPIATILGKSIDNPELRVAMPVTVAVLANWPGDALPDEAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHEEEEECCCCCCCCHHHH
FEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRKVPAEGPVREALISALPLWG
HHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
ELSTWQSLQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LLCVTLGYPLAYWLAKQPTGRANLLLMLVLLPFWTSLIVRTASWIVLLQSSGLINQSLLG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
SRLIEQPLALVFNRAGVYISMTHILLPFIVLPLYAVMKGISPNYVRAAISLGAHPFAAFW
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
RVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVSYFVAFFTNTTMNWGMAAAL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
GSQLLIIVLLLYVVYIRVTRTQAETAIH
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]