Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 22124416

GI number: 22124416

Start: 561662

End: 562972

Strand: Reverse

Name: purD [H]

Synonym: y0501

Alternate gene names: 22124416

Gene position: 562972-561662 (Counterclockwise)

Preceding gene: 22124417

Following gene: 22124410

Centisome position: 12.24

GC content: 51.87

Gene sequence:

>1311_bases
GTGCCCCTTCACGGAGTCATCCAGATGAATATTTTGATAATTGGTAACGGCGGTCGTGAACACGCTCTGGGCTGGAAAGC
CGCCCAATCTCCTTTAGCGGACAAAATTTATGTTGCACCAGGTAATGCGGGTACAGCACTGGAACCGACCTTAGAAAATG
TTGATATCGCCGCCACTGATATTGCCGGTTTACTGGCCTTTGCTCAAAGTCATGATATCGGCCTGACGATTGTTGGCCCA
GAAGCCCCTTTGGTGATCGGCGTGGTTGATGCGTTCCGCGCTGCTGGTTTAGCTATTTTTGGCCCGACTCAGGCTGCGGC
TCAATTAGAGGGTTCTAAAGCCTTCACCAAAGATTTCCTGGCCCGTCACAACATTCCCTCTGCGGAATACCAAAACTTTA
CAGATGTCGAGGCCGCATTGGCCTATGTGCGTCAAAAAGGTGCGCCAATCGTTATCAAAGCCGATGGTCTGGCCGCCGGT
AAAGGCGTGATTGTTGCGATGACGCAGGAAGAAGCCGAAACCGCCGTGAATGATATGTTGGCCGGTAACGCTTTTGGTGA
TGCAGGGCACCGTATCGTGGTGGAAGAGTTCCTTGATGGCGAAGAAGCCAGCTTTATCGTGATGGTTGATGGCGAAAATG
TTTTGCCAATGGCGACCAGTCAGGATCATAAGCGAGTTGGCGATGGTGATACCGGGCCAAATACCGGCGGAATGGGTGCT
TATTCCCCAGCCCCCGTGGTAACAGATGATGTTCACCAACGGGTCATGGATCAGGTTATTTGGCCGACCGTGCGTGGTAT
GGCGGCGGAAGGTAATATTTACACCGGTTTCCTCTATGCTGGCCTGATGATTTCAGCCGATGGGCAACCCAAAGTCATTG
AGTTCAACTGCCGCTTTGGCGATCCAGAAACGCAGCCAATCATGTTGCGTATGCGCTCCGATTTGGTCGAACTGTGTTTA
GCCGGTACACAAGGCAAACTAAATGAAAAAACCTCAGACTGGGATGAGCGCCCATCACTGGGGGTCGTTTTAGCCGCTGG
CGGTTATCCAGCAGATTACCGCCAGGGTGATGTTATTCATGGCTTACCACAGCAAGAAGTCAAGGATGGAAAAGTCTTCC
ACGCGGGGACCAAGCTGAATGGGAATCATGAAGTTGTCACCAATGGTGGCCGCGTCTTGTGTGTCACTGCACTCGGTGAA
ACCGTCGCGCAGGCGCAACAATATGCCTATCAGTTAGCTGAGGGGATCCAGTGGGAAGGGGTTTTCTGCCGTAAAGATAT
TGGTTATCGAGCGATTGCTCGCGGTAAGTAA

Upstream 100 bases:

>100_bases
TCGCGGCTGCTGATGAACACAGTATTGCCATGATCTTCACCGACATGCGCCATTTCCGTCATTAATTAACCCTCTTATAT
AGGAAGGGGCGCTTGATGAT

Downstream 100 bases:

>100_bases
AGGCTCACTGTCTTTAAAAGGGTACAGTCTAGTGCGCTGTGCCCTACTTATTGCGGATCATAATCGATTACTGGCTATCT
TATGACGCTGCACCGCTGAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPLHGVIQMNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGP
EAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAG
KGVIVAMTQEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGA
YSPAPVVTDDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCL
AGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGE
TVAQAQQYAYQLAEGIQWEGVFCRKDIGYRAIARGK

Sequences:

>Translated_436_residues
MPLHGVIQMNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGP
EAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAG
KGVIVAMTQEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGA
YSPAPVVTDDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCL
AGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGE
TVAQAQQYAYQLAEGIQWEGVFCRKDIGYRAIARGK
>Mature_435_residues
PLHGVIQMNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPE
APLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGK
GVIVAMTQEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAY
SPAPVVTDDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLA
GTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGET
VAQAQQYAYQLAEGIQWEGVFCRKDIGYRAIARGK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=80.7511737089202, Blast_Score=713, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=40.9836065573771, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.369020501139, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.1860465116279, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.1860465116279, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46194; Mature: 46063

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHGVIQMNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATD
CCCCEEEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHH
IAGLLAFAQSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFL
HHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHH
ARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTQEEAETAVNDML
HHCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHH
AGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGA
CCCCCCCCCCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
YSPAPVVTDDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFG
CCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEEECC
DPETQPIMLRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIH
CCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEC
GLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEG
CCCHHHCCCCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECE
VFCRKDIGYRAIARGK
EEEECCCCCEEECCCC
>Mature Secondary Structure 
PLHGVIQMNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATD
CCCEEEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHH
IAGLLAFAQSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFL
HHHHHHHHCCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHH
ARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTQEEAETAVNDML
HHCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHH
AGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGA
CCCCCCCCCCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
YSPAPVVTDDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFG
CCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEEECC
DPETQPIMLRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIH
CCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEC
GLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEG
CCCHHHCCCCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECE
VFCRKDIGYRAIARGK
EEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]