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The map label for this gene is purH [H]

Identifier: 22124417

GI number: 22124417

Start: 563008

End: 564642

Strand: Reverse

Name: purH [H]

Synonym: y0502

Alternate gene names: 22124417

Gene position: 564642-563008 (Counterclockwise)

Preceding gene: 22124420

Following gene: 22124416

Centisome position: 12.27

GC content: 51.01

Gene sequence:

>1635_bases
TTGATAATGAAGAAAACTGCGACTCCAAATCCAAGGGATAAAACCATGCAACAACGCCGTCCAATCCGCCGTGCTCTACT
CAGTGTGTCTGACAAAGCAGGTATCATCGAATTCGCCCAAGCACTTTCTCAACGCGGTATCGAGTTACTTTCCACCGGTG
GGACTGCCCGCCTGCTGGCTGATGCTGGTTTACCCGTTACCGAAGTGTCTGACTACACCGGTTTCCCGGAAATGATGGAT
GGACGTGTGAAGACTTTGCATCCAAAGGTGCATGGTGGGATTTTAGGTCGTCGTGGTCAGGATGATGGCATCATGGCTCA
ACATGGCATTCAGCCCATTGATATTGTCGTCGTTAATTTATATCCCTTCGCCCAGACGGTTGCCCGCCCGGATTGCTCGT
TGGAAGATGCGGTTGAGAATATTGATATTGGTGGCCCAACCATGGTTCGCTCTGCGGCCAAGAACCATAAAGATGTCGCC
ATCGTGGTGAAGAGTAGCGACTACCCCGCCATTATTACTGAGCTTGATAATAATGATGGTTCGTTGACTTACCCCACCCG
CTTCAATCTGGCCATTAAAGCTTTCGAACACACCGCCGCCTACGACAGCATGATCGCCAACTACTTCGGCACACTGGTGC
CACCTTATCATGGTGATACGGAACAGCCTTCCGGCCACTTCCCTCGCACCCTAAATCTTAACTATATAAAGAAGCAGGAT
ATGCGTTACGGTGAAAACAGCCACCAGCAAGCTGCCTTCTATATAGAAGAAGATGTCAAAGAGGCATCCGTTGCCACTGC
CCAGCAATTACAAGGGAAAGCCCTCTCTTATAACAATATTGCGGATACCGATGCCGCGCTGGAATGCGTGAAAGAGTTCA
GTGAACCAGCCTGTGTGATCGTTAAACATGCCAACCCATGCGGTGTGGCTATCGGTGATTCTATTCTTGCCGCTTATGAA
CGTGCCTATCAAACCGATCCAACCTCAGCTTTCGGTGGCATCATCGCCTTTAACCGTGAATTGGATGCAGCAACGGCCAG
CGCGATCATCAGCCGCCAGTTTGTCGAAGTGATCATTGCGCCAACAGTCAGCTCTGATGCATTGGCATTGCTTGCAGCTA
AACAAAATGTCCGAGTCCTGACTTGTGGCCAGTGGCAAGCACGTTCAGCAGGTTTAGATTTCAAACGTGTTAATGGGGGT
TTGCTGGTACAAGAACGCGATTTAGGTATGGTGACGGCGGCCGACCTTCGCGTGGTTTCCAAGCGTCAGCCTACCGAACA
GGAACTGCGTGATGCGCTGTTCTGCTGGAAAGTGGCTAAGTTTGTTAAATCCAATGCGATTGTCTATGCCCGCGATAACA
TGACAATCGGTATAGGTGCCGGCCAAATGAGCCGCGTGTACTCTGCGAAAATAGCCGGTATCAAGGCCGCAGATGAAGGG
CTGGAAGTGGCTGGCTCAGCCATGGCCTCTGATGCCTTCTTCCCGTTCCGTGATGGTATTGATGCCGCCGCGGCTGTGGG
CATTACTTGTGTCATCCAACCGGGCGGCTCAATTCGTGATGATGAAGTCATCGCGGCTGCTGATGAACACAGTATTGCCA
TGATCTTCACCGACATGCGCCATTTCCGTCATTAA

Upstream 100 bases:

>100_bases
TTCGCTACAATTCCCCGCGTCGAAAAGGGTGACCTCATCGACAAAATATCAACGAAATAGTCTGTAGCGCCCTATTAATT
GATAGTGAAGAACTCTGTAA

Downstream 100 bases:

>100_bases
TTAACCCTCTTATATAGGAAGGGGCGCTTGATGATGTGCCCCTTCACGGAGTCATCCAGATGAATATTTTGATAATTGGT
AACGGCGGTCGTGAACACGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MIMKKTATPNPRDKTMQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMD
GRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVA
IVVKSSDYPAIITELDNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQD
MRYGENSHQQAAFYIEEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYE
RAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGG
LLVQERDLGMVTAADLRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEG
LEVAGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH

Sequences:

>Translated_544_residues
MIMKKTATPNPRDKTMQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMD
GRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVA
IVVKSSDYPAIITELDNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQD
MRYGENSHQQAAFYIEEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYE
RAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGG
LLVQERDLGMVTAADLRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEG
LEVAGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH
>Mature_544_residues
MIMKKTATPNPRDKTMQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMD
GRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVA
IVVKSSDYPAIITELDNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQD
MRYGENSHQQAAFYIEEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYE
RAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGG
LLVQERDLGMVTAADLRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEG
LEVAGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=38.6831275720165, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=87.1455576559546, Blast_Score=962, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=620, Percent_Identity=34.6774193548387, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI71985574, Length=358, Percent_Identity=28.4916201117318, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=36.5217391304348, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.0082304526749, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=623, Percent_Identity=31.3001605136437, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24649832, Length=612, Percent_Identity=35.7843137254902, Blast_Score=308, Evalue=7e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 58869; Mature: 58869

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKKTATPNPRDKTMQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEE
DAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNL
CCCCCCEECCCCCCCHHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCEEEEEEEEE
YPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITELDNNDG
EHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCC
SLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQD
CEECCEEECEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHH
MRYGENSHQQAAFYIEEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVI
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
VKHANPCGVAIGDSILAAYERAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIA
EECCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEEE
PTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAADLRVVS
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHH
KRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEG
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCC
LEVAGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMR
HHHHHHHHHCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEEHHH
HFRH
HHCC
>Mature Secondary Structure
MIMKKTATPNPRDKTMQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEE
DAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNL
CCCCCCEECCCCCCCHHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCEEEEEEEEE
YPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITELDNNDG
EHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCC
SLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQD
CEECCEEECEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHH
MRYGENSHQQAAFYIEEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVI
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEE
VKHANPCGVAIGDSILAAYERAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIA
EECCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEEE
PTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAADLRVVS
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHH
KRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEG
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCC
LEVAGSAMASDAFFPFRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMR
HHHHHHHHHCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEEHHH
HFRH
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA