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The map label for this gene is rffH [H]

Identifier: 22124282

GI number: 22124282

Start: 410474

End: 411565

Strand: Direct

Name: rffH [H]

Synonym: y0367

Alternate gene names: 22124282

Gene position: 410474-411565 (Clockwise)

Preceding gene: 22124281

Following gene: 22124283

Centisome position: 8.92

GC content: 48.81

Gene sequence:

>1092_bases
TTGCTTGCCGCCTTCCTGCACCTTGAAATCTATAGTGTCTATCGTTGGGCGACCTACGAGAGACACCAAAGTCGATTAAT
CATCGGGATGGCGTCATCCAGTCGTTTGCATGATGTTTTGCCTGTGTATAGCCTCTGCCTGATGGTCTCAGGAGATGGTA
TGTATCAGGAAATTGTATGTATCAGGAGATTGCATGTATCAGGAGAACGTATGAAAGGCATAATTCTGGCTGGTGGTTCA
GGTTCCCGATTACACCCCATCACCCGTGGCGTCTCTAAGCAGTTGCTTCCCATCTACGATAAACCCATGATCTATTACCC
GCTGTCAGTATTGATGCTGGCAGGGATCCGTGACGTGTTGATTATCTCAACCCCGGAAGACTTACCATCGTTTCAGCGTT
TGTTGGGCAACGGTGATGAGTTTGGTATTAATCTGAGTTATGCCGCGCAGCCTAGCCCAGACGGGCTGGCACAGGCATTT
ATCATTGGTGAAGCGTTTATTGATAATGAGCCATGCTGTTTGGTGCTCGGTGATAATATCTACTTCGGCCAAGGCTTTAG
TCCAAAACTGAAAGCTGTCGCCGCGCGTCAGCAGGGCGCGACTGTTTTCGGCTATCAGGTGATGGATCCAGAGCGCTTTG
GCGTAGTGGAGTTTGATGACAACTTCCGCGCGTTGTCTATTGAAGAAAAACCGAGCCAGCCAAAATCAAATTGGGCGGTG
ACCGGGCTCTATTTCTACGATAACCAAGTGGTTGATTTTGCCAAGCAGGTTAAACCTTCTGCTCGCGGTGAACTGGAAAT
TACCAGCATTAATCAAATGTATCTGGATCGCGGTGAGTTAACGGTTGAGCTGCTAGGGCGCGGTTTTGCTTGGCTGGATA
CCGGCACCCATGACAGCCTGATCGAAGCCAGTACTTTTGTTCAAACGGTTGAAAAACGCCAAGGTTTTAAAATTGCTTGT
TTGGAAGAGATTGCCTGGCGTAACGGTTGGTTGGATGATGATGGCGTAAAACGTGCGGCAACCGCGCTGGCGAAAACCGG
TTATGGCAAATATCTGTTGGATCTATTACATGCCCGTCCACGCCAGTATTGA

Upstream 100 bases:

>100_bases
GGGCTTGAGTGACTAACACCCGTCATTTTTCACGTTGTAGGTGTATTGGCTGCGCTCAGTCATCCGAATAACTTACTGAT
GTAAGCACCTCGGGGTTCTC

Downstream 100 bases:

>100_bases
GCCACTGGGCTGGGAAAGTGAATTTTTCCAGCGCCAAAGTGCAAAATTGATTTTTTCCGACTCAGCCCCGCCACTTAACC
CGGCTGAGTTGGCTGCTTTT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MLAAFLHLEIYSVYRWATYERHQSRLIIGMASSSRLHDVLPVYSLCLMVSGDGMYQEIVCIRRLHVSGERMKGIILAGGS
GSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQPSPDGLAQAF
IIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAV
TGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTVEKRQGFKIAC
LEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY

Sequences:

>Translated_363_residues
MLAAFLHLEIYSVYRWATYERHQSRLIIGMASSSRLHDVLPVYSLCLMVSGDGMYQEIVCIRRLHVSGERMKGIILAGGS
GSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQPSPDGLAQAF
IIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAV
TGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTVEKRQGFKIAC
LEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY
>Mature_363_residues
MLAAFLHLEIYSVYRWATYERHQSRLIIGMASSSRLHDVLPVYSLCLMVSGDGMYQEIVCIRRLHVSGERMKGIILAGGS
GSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVLIISTPEDLPSFQRLLGNGDEFGINLSYAAQPSPDGLAQAF
IIGEAFIDNEPCCLVLGDNIYFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAV
TGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSLIEASTFVQTVEKRQGFKIAC
LEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARPRQY

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790224, Length=293, Percent_Identity=86.6894197952219, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=65.4929577464789, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI133931050, Length=229, Percent_Identity=24.8908296943231, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=277, Percent_Identity=26.3537906137184, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355443, Length=254, Percent_Identity=24.4094488188976, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644084, Length=254, Percent_Identity=24.4094488188976, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 40579; Mature: 40579

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAFLHLEIYSVYRWATYERHQSRLIIGMASSSRLHDVLPVYSLCLMVSGDGMYQEIVC
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHH
IRRLHVSGERMKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVL
HHHHHCCCCCEEEEEEECCCCCCCCHHHHCCHHCCCCCCCCCEEEHHHHHHHHCCCCEEE
IISTPEDLPSFQRLLGNGDEFGINLSYAAQPSPDGLAQAFIIGEAFIDNEPCCLVLGDNI
EEECCCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHHHEEEEEHHHCCCCCEEEEECCEE
YFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAV
EECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCEEE
TGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSL
EEEEEECCHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCEEEEEECCCEEEEECCCCCHH
IEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARP
HHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
RQY
CCC
>Mature Secondary Structure
MLAAFLHLEIYSVYRWATYERHQSRLIIGMASSSRLHDVLPVYSLCLMVSGDGMYQEIVC
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHH
IRRLHVSGERMKGIILAGGSGSRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIRDVL
HHHHHCCCCCEEEEEEECCCCCCCCHHHHCCHHCCCCCCCCCEEEHHHHHHHHCCCCEEE
IISTPEDLPSFQRLLGNGDEFGINLSYAAQPSPDGLAQAFIIGEAFIDNEPCCLVLGDNI
EEECCCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHHHEEEEEHHHCCCCCEEEEECCEE
YFGQGFSPKLKAVAARQQGATVFGYQVMDPERFGVVEFDDNFRALSIEEKPSQPKSNWAV
EECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCEEE
TGLYFYDNQVVDFAKQVKPSARGELEITSINQMYLDRGELTVELLGRGFAWLDTGTHDSL
EEEEEECCHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCEEEEEECCCEEEEECCCCCHH
IEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDDGVKRAATALAKTGYGKYLLDLLHARP
HHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
RQY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]