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The map label for this gene is rffG [H]

Identifier: 22124281

GI number: 22124281

Start: 409316

End: 410389

Strand: Direct

Name: rffG [H]

Synonym: y0366

Alternate gene names: 22124281

Gene position: 409316-410389 (Clockwise)

Preceding gene: 22124280

Following gene: 22124282

Centisome position: 8.9

GC content: 51.3

Gene sequence:

>1074_bases
ATGGCGTTGAGGCGAATTCTCGTCACAGGCGGGGCCGGTTTTATCGGCTCTGCGGTGGTCAGGCATATTATTGATGGCAC
CTCAGATAGCGTGGTGGTAGTCGATAAACTCACCTATGCCGGTAATCTGGAGTCGCTGTCAGTGGTTGCGGGCAGTGAGC
GTTATGCTTTCGAACAAGTCGATATCTGTGACAGCAGTGAGTTGGATCGGGTTTTCGCACAGTATCAGCCGAATGTGGTG
ATGCATTTGGCTGCCGAGAGCCACGTTGACCGCTCAATTGATGGCCCCGCCGCGTTTATCGAGACGAACGTGGTTGGCAC
TTACACCCTGTTGGAAGCTGCCCGTCACTATTGGCAACAACTGAGTGTTGAAGCCAAGCAGGCGTTCCGCTTTCACCATA
TCTCAACGGATGAGGTGTATGGCGATTTACACGGCACTGACGATTTGTTTACCGAAACCACGCCGTATGCGCCAAGCAGC
CCATATTCGGCGTCTAAAGCGTCCAGTGACCATTTAGTCCGGGCTTGGTTGCGTACTTATGGTTTACCGACTTTGGTGAC
TAATTGTTCAAATAATTACGGCCCGTATCACTTCCCAGAAAAGCTGATCCCGCTGGTTATTTTAAATGCGTTGGCGGGTA
AGCCGCTGCCTGTTTATGGCAATGGTGCTCAGGTCCGCGACTGGTTGTATGTAGAAGACCACGCCAGAGCGTTGTATCAG
GTGGTTACTGAGGGTGTGGTGGGTGAAACCTACAATATCGGCGGCCATAATGAGCGCAAGAATATTGAAGTAGTTGAAAC
CATTTGCGCGCTATTGGATGAGTTGGTGCCAGCAAAACCGGCGGGTATCGCACACTACCGTGATTTGATTACCTACGTAA
AAGACCGGCCGGGTCATGATATGCGCTATGCCATTGATGCTGGCAAGATTGAGCGGGAACTGGGTTGGCGTCCACAAGAA
ACCTTCGAAAGCGGTATCCGTAAAACGGTCTTGTGGTATTTGAATAATGAATCTTGGTGGCGTCGTGTTCAGGATGGCTC
TTACGCCGGAGAGCGTTTGGGCTTGAGTGACTAA

Upstream 100 bases:

>100_bases
AACAGGCTGATGTCTTGGTCATGTTGGTGGACCACAAACAATTCAAGGCCATCAAACCTGAAGATATTAAGCAACAGTGG
ATTGTTGATACCAAAGGAGT

Downstream 100 bases:

>100_bases
CACCCGTCATTTTTCACGTTGTAGGTGTATTGGCTGCGCTCAGTCATCCGAATAACTTACTGATGTAAGCACCTCGGGGT
TCTCTTGCTTGCCGCCTTCC

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MALRRILVTGGAGFIGSAVVRHIIDGTSDSVVVVDKLTYAGNLESLSVVAGSERYAFEQVDICDSSELDRVFAQYQPNVV
MHLAAESHVDRSIDGPAAFIETNVVGTYTLLEAARHYWQQLSVEAKQAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSS
PYSASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQVRDWLYVEDHARALYQ
VVTEGVVGETYNIGGHNERKNIEVVETICALLDELVPAKPAGIAHYRDLITYVKDRPGHDMRYAIDAGKIERELGWRPQE
TFESGIRKTVLWYLNNESWWRRVQDGSYAGERLGLSD

Sequences:

>Translated_357_residues
MALRRILVTGGAGFIGSAVVRHIIDGTSDSVVVVDKLTYAGNLESLSVVAGSERYAFEQVDICDSSELDRVFAQYQPNVV
MHLAAESHVDRSIDGPAAFIETNVVGTYTLLEAARHYWQQLSVEAKQAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSS
PYSASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQVRDWLYVEDHARALYQ
VVTEGVVGETYNIGGHNERKNIEVVETICALLDELVPAKPAGIAHYRDLITYVKDRPGHDMRYAIDAGKIERELGWRPQE
TFESGIRKTVLWYLNNESWWRRVQDGSYAGERLGLSD
>Mature_356_residues
ALRRILVTGGAGFIGSAVVRHIIDGTSDSVVVVDKLTYAGNLESLSVVAGSERYAFEQVDICDSSELDRVFAQYQPNVVM
HLAAESHVDRSIDGPAAFIETNVVGTYTLLEAARHYWQQLSVEAKQAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSP
YSASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQVRDWLYVEDHARALYQV
VTEGVVGETYNIGGHNERKNIEVVETICALLDELVPAKPAGIAHYRDLITYVKDRPGHDMRYAIDAGKIERELGWRPQET
FESGIRKTVLWYLNNESWWRRVQDGSYAGERLGLSD

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=35.1744186046512, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=26.6272189349112, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI4504031, Length=286, Percent_Identity=25.1748251748252, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI56237023, Length=300, Percent_Identity=27.6666666666667, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI56118217, Length=300, Percent_Identity=27.6666666666667, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI189083684, Length=300, Percent_Identity=27.6666666666667, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=82.4362606232295, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=74.7159090909091, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=25.2124645892351, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788366, Length=293, Percent_Identity=26.6211604095563, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=348, Percent_Identity=34.4827586206897, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=32.6283987915408, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=341, Percent_Identity=24.633431085044, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=24.8587570621469, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=25.5681818181818, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=292, Percent_Identity=23.972602739726, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=25.1381215469613, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356223, Length=354, Percent_Identity=26.271186440678, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19923002, Length=374, Percent_Identity=25.4010695187166, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24158427, Length=277, Percent_Identity=27.4368231046931, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39820; Mature: 39689

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRRILVTGGAGFIGSAVVRHIIDGTSDSVVVVDKLTYAGNLESLSVVAGSERYAFEQV
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
DICDSSELDRVFAQYQPNVVMHLAAESHVDRSIDGPAAFIETNVVGTYTLLEAARHYWQQ
CCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LSVEAKQAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPYSASKASSDHLVRAWLRTY
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQVRDWLYVEDHARALYQ
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEHHHHHHHHH
VVTEGVVGETYNIGGHNERKNIEVVETICALLDELVPAKPAGIAHYRDLITYVKDRPGHD
HHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
MRYAIDAGKIERELGWRPQETFESGIRKTVLWYLNNESWWRRVQDGSYAGERLGLSD
EEEEECCHHHHHHHCCCCHHHHHHCHHEEEEEEECCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALRRILVTGGAGFIGSAVVRHIIDGTSDSVVVVDKLTYAGNLESLSVVAGSERYAFEQV
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
DICDSSELDRVFAQYQPNVVMHLAAESHVDRSIDGPAAFIETNVVGTYTLLEAARHYWQQ
CCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LSVEAKQAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPYSASKASSDHLVRAWLRTY
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GLPTLVTNCSNNYGPYHFPEKLIPLVILNALAGKPLPVYGNGAQVRDWLYVEDHARALYQ
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEHHHHHHHHH
VVTEGVVGETYNIGGHNERKNIEVVETICALLDELVPAKPAGIAHYRDLITYVKDRPGHD
HHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
MRYAIDAGKIERELGWRPQETFESGIRKTVLWYLNNESWWRRVQDGSYAGERLGLSD
EEEEECCHHHHHHHCCCCHHHHHHCHHEEEEEEECCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]