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The map label for this gene is nth [H]

Identifier: 21910178

GI number: 21910178

Start: 703509

End: 704165

Strand: Direct

Name: nth [H]

Synonym: SpyM3_0642

Alternate gene names: 21910178

Gene position: 703509-704165 (Clockwise)

Preceding gene: 21910177

Following gene: 21910179

Centisome position: 37.02

GC content: 39.57

Gene sequence:

>657_bases
ATGAGAATTGGAAAAGCAAGATTAGCAAAAGTCCTGACCATCATTGGTCAAATGTTTCCTGAGGCTAAGGGAGAGTTAGA
CTGGGAAACACCTTTTCAATTGTTGATTGCGGTGATTTTATCAGCTCAAACGACAGATAAGGCGGTCAATAAGGTAACTC
CTGGACTATGGCAGTCTTATCCAGAAATAGAAGACTTAGCTTTTGCTGAACTTTCTGATGTTGAAAATGCTCTGAGAACG
ATTGGACTCTATAAAAATAAGGCCAAAAATATCATTAAAACTGCTCAGGCTATTCGTGATGATTTTAAGGGTCAAGTGCC
AAAAACCCACAAAGAGCTTGAAAGTTTACCGGGTGTTGGCCGAAAAACAGCCAATGTGGTATTGGCAGAGGTTTATGGTG
TTCCAGCTATTGCGGTTGATACTCATGTGGCTAGAGTCTCAAAGAGGCTCAATATTTCATCACCAGATGCCGATGTCAAG
CAGATTGAAGCAGATTTGATGGCTAAAATTCCAAAGAAAGATTGGATTATTACTCACCATCGATTGATTTTCTTTGGACG
CTACCATTGTTTAGCCAAAAAACCAAAATGTGAGATTTGTCCCGTTCAGTCTTACTGCAAGTACTATCAAAATACTTATG
GAAAATCGAAAGCCTAG

Upstream 100 bases:

>100_bases
GACAAGTAGAAGAACGTAGACGTGTTAGAGAAGGCGAAGATCTTTCTCAAGTGACGATATCAGAGGATTTTCTATCTGCG
ATGAATTTATGGAGTGATTC

Downstream 100 bases:

>100_bases
CCTTTGGTTTTCTTTTTTTATGGAGGTAATTTTGCTATAATAGAACCATTACGTTTTAGAAAGAAATCAAGATGGATAGT
CAATTATCAAACCGTTTAGC

Product: putative endonuclease III (DNA repair)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQTTDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRT
IGLYKNKAKNIIKTAQAIRDDFKGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA

Sequences:

>Translated_218_residues
MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQTTDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRT
IGLYKNKAKNIIKTAQAIRDDFKGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
>Mature_218_residues
MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQTTDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRT
IGLYKNKAKNIIKTAQAIRDDFKGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=186, Percent_Identity=29.0322580645161, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI115298648, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI190358497, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI6912520, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298650, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298654, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298652, Length=196, Percent_Identity=26.0204081632653, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=42.3076923076923, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789331, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=37.4301675977654, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=28.8770053475936, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=29.8850574712644, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=184, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24423; Mature: 24423

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQTTDKAVNKVTPGLWQSY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHCC
PEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDFKGQVPKTHKELESLPGVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
RKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVKQIEADLMAKIPKKDWIITHH
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
RLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQTTDKAVNKVTPGLWQSY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHCC
PEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDFKGQVPKTHKELESLPGVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
RKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVKQIEADLMAKIPKKDWIITHH
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHH
RLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]