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The map label for this gene is trmD [H]

Identifier: 21910110

GI number: 21910110

Start: 631792

End: 632523

Strand: Direct

Name: trmD [H]

Synonym: SpyM3_0574

Alternate gene names: 21910110

Gene position: 631792-632523 (Clockwise)

Preceding gene: 21910109

Following gene: 21910111

Centisome position: 33.24

GC content: 41.26

Gene sequence:

>732_bases
ATGAAGATTGATATTTTAACCCTTTTTCCTGAAATGTTTGCTCCCCTAGAACATTCCATTGTAGGCAAGGCAAAAGAAAA
AGGTCTGTTAGACATTCACTACCATAACTTTCGAGATTATGCTGAAAAAGCTCGTCATGTGGATGACGAACCCTATGGTG
GGGGCCAAGGCATGTTATTGAGAGCGCAGCCCATTTTTGACACCATTGAACAGATTGAAGCCAAAAAACCAAGGATTATT
CTTTTAGATCCAGCAGGTAAACCCTTTACTCAGGCTTATGCTGAAGAATTAGCTCTTGAGGAAGAGTTAATTTTTATTTG
TGGTCATTATGAAGGTTATGATGAGCGGATTAAGACCTTGGTGACGGATGAAATTTCATTAGGCGACTTTGTATTAACGG
GAGGTGAGTTGGCGGCCATGACTATGGTGGATGCGACCGTCAGGTTAATTCCACAGGTACTTGGGAAAGAAAGTAGCCAT
CAGGACGATTCTTTTTCATCAGGACTTTTAGAATACCCTCAATACACAAGACCTTATGATTATCGTGGTATGACAGTTCC
AGATGTTCTAATGAGTGGTCATCACGAACGTATTCGCTTGTGGCGTCTAGAAGAAAGTCTCAGAAAAACCTATTTGAGAA
GGCCAGATTTGTTAGAGCGGTACGATTTTTCCGAGGAAGAGCGCAAGCTATTGGATAAGATTAAAGAAGCATTGGGTCAA
GGAGAAGATTAG

Upstream 100 bases:

>100_bases
GGAAAACGAGACTTGCTCTTACCATATATTCCCCCAGTAGTTCTTAACGTAGATGTTCCTAACAAGCGCGTGGATGTTGA
ATTGATGGAAGGCTTAGACG

Downstream 100 bases:

>100_bases
TGAAAGATAAAGCATATGATATTACGATTATTGGTGGTGGTCCAATAGGTTTATTTGCAGCCTTTTATGCAGGTCTTAGG
GGCGTAACAGTAAAGATTAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLLERYDFSEEERKLLDKIKEALGQ
GED

Sequences:

>Translated_243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLLERYDFSEEERKLLDKIKEALGQ
GED
>Mature_243_residues
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRII
LLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLLERYDFSEEERKLLDKIKEALGQ
GED

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=48.9626556016597, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27979; Mature: 27979

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLL
CEEEHEEHHHHHHCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTL
ECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLLERYDFSEEERKLLDKIKEALGQ
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCC
GED
CCC
>Mature Secondary Structure
MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKARHVDDEPYGGGQGMLL
CEEEHEEHHHHHHCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEE
RAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEELIFICGHYEGYDERIKTL
ECCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLLERYDFSEEERKLLDKIKEALGQ
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCC
GED
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]