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The map label for this gene is ligA [H]

Identifier: 21910027

GI number: 21910027

Start: 542138

End: 544096

Strand: Direct

Name: ligA [H]

Synonym: SpyM3_0491

Alternate gene names: 21910027

Gene position: 542138-544096 (Clockwise)

Preceding gene: 21910026

Following gene: 161485751

Centisome position: 28.53

GC content: 40.48

Gene sequence:

>1959_bases
ATGAAAAAACGCATAAAAGAATTAACAGATCTTCTTAACCGTTACCGTTATGACTATTATACTAAAGATGCCCCATCAGT
TTCTGACAGTGACTATGATAAGCTTTATCGTGAACTGGTGACTTTAGAGCAGTCATATCCCGAGTATGTCTTGCAAGATA
GCCCAACGCAGCAGGTTGGAGGGACTATTTTAAAGGGGTTTGAAAAATATCGACATCAGTATCCGCTTTTTAGTTTGCAG
GATGCTTTTTCACGTGAGGAGCTGGATGCTTTTGATAAGCGCGTCAAGGCCGAATTTCCGAATGCCACCTATCTAGCAGA
GTTAAAAATTGATGGTTTATCTATTTCCTTGAGTTATGAGAATGGCTTCTTACAGGTTGGAGCAACACGGGGTGATGGCA
ATATCGGTGAAAATATTACAGAAAATATCAAAAAAATAAAGGATATTCCTCACCAATTGAGTGAGCCTTTAACCATCACC
GTTCGAGGAGAGGCTTACATGTCTCGTCAGTCCTTTAAAGCGATCAATGAAGGACGCCAAGAAAATGGGGAGACTGAATT
TGCTAATCCTAGAAATGCTGCCGCTGGGACACTAAGGCAGCTAGATACGGCAGTGGTTGCTAAGCGTCAACTAGCCACTT
TTTTGTATCAAGAAGTTAGTCCGACAGCTAGAAATCAACAAAATGAGGTCTTAGCAGAATTAGCAGATCTAGGTTTTTCA
GTAAATCCGTATTATCAACTCACATCGTCTATGGATGAGATTTGGGATTTTATCAAAACTATTGAGGCAAAAAGAGATCA
ATTAGCTTATGACATAGATGGCGTTGTTATTAAAGTTAATAGTCTAGCCATGCAAGAAGAGCTAGGGTTTACGGTTAAAG
CTCCACGCTGGGCCATTGCTTACAAATTTCCAGCAGAAGAAAAAGAGGCAGAAATCCTTTCGGTTGACTGGACAGTGGGA
CGAACAGGCGTGGTCACACCTACAGCGAATCTAACTCCTGTGCAACTAGCGGGGACAACCGTGAGTCGTGCAACCCTACA
TAATGTGGATTATATTGCCGAAAAAGATATCCGTATTGGCGATACAGTGATTGTATATAAGGCAGGGGACATTATTCCTG
CTGTCTTAAATGTCGTTATGAGTAAGCGGAACCAGCAAGAAGTCATGCTTATTCCTAAACTATGCCCATCTTGTGGTAGT
GAATTAGTTCATTTTGAAGATGAGGTTGCTTTGCGTTGTATTAATCCGTTGTGTCCTAGTTTGATTCAGCGAAGTTTGGA
GCATTTTGCAAGTCGTGATGCCATGAACATTACTGGGCTGGGACCAGCTATTGTGGAAAAACTATTTTTAGCGGGGTTTG
TTCATGATGTTGCTGATATTTACCAGTTGACCAAAGAAGATTTCATGCAATTAGATGGCATCAAAGAAAAGTCAGCTGAT
AAATTGCTCGCTGCGATTGAGGCTTCTAAATCAAATTCAGCTGAAAAACTGTTATTTGGTCTAGGAATTCGTCATATTGG
CTCTAAAGTTAGTCGCTTAATTTTAGAGGTATACGGAGATATTTCTGCTCTTTTAACCGCTAAAGAAGAGGAAATCGCAA
GGATAGATGGCCTTGGCTCAACGATTGCTCAATCACTTACTCAATACTTTGAACAAAAAACGGCTGCGATTTTAGTAGAC
GAGTTAAAGACAGCAGGTGTCAACATGCATTACTCAGGTCAAAAAGTTAATAGTGACGCAGCGCTTTTTGGTCTAACTGT
TGTTTTAACTGGCAAATTAAATCAGCTGAACCGAAATGAGGCCAAAGATAAATTAGAGGCATTAGGTGCCAAAGTTACTG
GAAGTGTATCTAAGAAAACAGATTTAGTTATTGCAGGTAGTGACGCTGGTTCTAAGCTAGAAAAAGCAAAGAGCTTAGGA
ATTCGGATTGAAGATGAAGATTGGCTACGCCAGCTTTAA

Upstream 100 bases:

>100_bases
TTTAGCTAATAGTACCATATGGAGTGGCCAACGATTACTTTTGATGTTGTTATGATATAGAAAATAGCTCATTTGTTATC
TTTTATGATAAAATAAAAGT

Downstream 100 bases:

>100_bases
GTGAGGTAATGTTATGAAAAAACAATTGAGAGCACGTCTCATTTATAATCCAACGTCAGGACAAGAACTTATGAGAAAAA
GTGTTCCTGAAGTTTTAGAC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEGRQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEVSPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Sequences:

>Translated_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEGRQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEVSPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL
>Mature_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEGRQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEVSPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=42.6448736998514, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI87082305, Length=558, Percent_Identity=22.5806451612903, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 72393; Mature: 72393

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTITVRGEAYMSRQSFKAINEGRQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEVSPTARNQQNEVLAELADLGFS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC
>Mature Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTITVRGEAYMSRQSFKAINEGRQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEVSPTARNQQNEVLAELADLGFS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]