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The map label for this gene is dagK [H]

Identifier: 161485751

GI number: 161485751

Start: 544110

End: 545132

Strand: Direct

Name: dagK [H]

Synonym: SpyM3_0492

Alternate gene names: 161485751

Gene position: 544110-545132 (Clockwise)

Preceding gene: 21910027

Following gene: 21910029

Centisome position: 28.63

GC content: 37.44

Gene sequence:

>1023_bases
ATGAAAAAACAATTGAGAGCACGTCTCATTTATAATCCAACGTCAGGACAAGAACTTATGAGAAAAAGTGTTCCTGAAGT
TTTAGACATATTAGAAGGGTTCGGGTATGAAACATCTGCCTTTCAAACAACAGCCCAAAAGAACTCTGCTCTAAATGAAG
CAAGAAGAGCGGCAAAGGCAGGTTTTGACTTATTGATTGCTGCTGGAGGTGATGGCACTATTAACGAAGTGGTCAATGGA
ATTGCTCCCTTGAAAAAGCGCCCTAAAATGGCCATTATTCCAACAGGTACCACTAATGATTTTGCTAGAGCGTTAAAAGT
ACCAAGAGGAAATCCGAGCCAAGCAGCAAAACTTATCGGAAAAAATCAGACTATCCAGATGGATATTGGTAGAGCTAAGA
AAGATACTTATTTCATCAATATTGCTGCAGCAGGAAGTTTGACGGAATTAACTTACAGTGTACCAAGTCAGTTAAAAACG
ATGTTTGGATACTTGGCTTATCTTGCTAAAGGAGTAGAACTGCTCCCAAGAGTTAGCAATGTGCCTGTTAAAATTACGCA
TGACAAAGGCGTCTTTGAAGGTCAAGTTTCTATGATTTTTGCAGCTATTACCAATTCTGTTGGGGGATTTGAAATGATTG
CTCCTGATGCAAAACTAGATGACGGTATGTTTACGCTCATTTTAATAAAGACAGCAAATCTTTTTGAAATTGTACATCTT
TTGCGTTTGATTTTGGATGGTGGTAAGCATATTACAGATCGCCGTGTCGAATACATTAAGACCAGTAAAATAGTCATTGA
ACCCCAATGTGGTAAGCGAATGATGATTAATTTAGATGGTGAGTACGGAGGAGATGCTCCAATTACATTAGAGAACCTAA
AAAATCATATCACTTTTTTTGCAGATACTGATTTGATTTCTGATGACGCTTTAGTCTTAGATCAGGACGAATTGGAAATT
GAGGAAATCGTAAAGAAATTTGCTCATGAAGTAGAAGACTTAGAACAAGAATTAGAAGAATAA

Upstream 100 bases:

>100_bases
GCAGGTAGTGACGCTGGTTCTAAGCTAGAAAAAGCAAAGAGCTTAGGAATTCGGATTGAAGATGAAGATTGGCTACGCCA
GCTTTAAGTGAGGTAATGTT

Downstream 100 bases:

>100_bases
TACAATAGTTATTAGCTACTGACAGTTTAGTCAAATGCACTACTATTAGAATCTAAGATTTTTAAATCAATTGAGGTAGT
GTTTTTTGATGCACTCAAAG

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTTAQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNG
IAPLKKRPKMAIIPTGTTNDFARALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAKLDDGMFTLILIKTANLFEIVHL
LRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMINLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEI
EEIVKKFAHEVEDLEQELEE

Sequences:

>Translated_340_residues
MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTTAQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNG
IAPLKKRPKMAIIPTGTTNDFARALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAKLDDGMFTLILIKTANLFEIVHL
LRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMINLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEI
EEIVKKFAHEVEDLEQELEE
>Mature_340_residues
MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTTAQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNG
IAPLKKRPKMAIIPTGTTNDFARALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAKLDDGMFTLILIKTANLFEIVHL
LRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMINLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEI
EEIVKKFAHEVEDLEQELEE

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=187, Percent_Identity=30.4812834224599, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 37443; Mature: 37443

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTTAQKNSALNEARRAAKA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFARALKVPRGNPSQAAKLIG
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHC
KNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKTMFGYLAYLAKGVELLPRVSN
CCCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAKLDDGMFTLILIKTANLFEIVHL
CCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHH
LRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMINLDGEYGGDAPITLENLKNHITFF
HHHHHCCCCCCHHHHHHHEEECEEEECCCCCCEEEEEECCCCCCCCCEEHHHHHCCEEEE
ADTDLISDDALVLDQDELEIEEIVKKFAHEVEDLEQELEE
EECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTTAQKNSALNEARRAAKA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFARALKVPRGNPSQAAKLIG
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHC
KNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKTMFGYLAYLAKGVELLPRVSN
CCCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAKLDDGMFTLILIKTANLFEIVHL
CCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEECCCHHHHHHH
LRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMINLDGEYGGDAPITLENLKNHITFF
HHHHHCCCCCCHHHHHHHEEECEEEECCCCCCEEEEEECCCCCCCCCEEHHHHHCCEEEE
ADTDLISDDALVLDQDELEIEEIVKKFAHEVEDLEQELEE
EECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]