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The map label for this gene is lctO [C]

Identifier: 21909833

GI number: 21909833

Start: 333160

End: 334341

Strand: Direct

Name: lctO [C]

Synonym: SpyM3_0297

Alternate gene names: 21909833

Gene position: 333160-334341 (Clockwise)

Preceding gene: 21909831

Following gene: 21909834

Centisome position: 17.53

GC content: 42.13

Gene sequence:

>1182_bases
ATGGCACAAAAAACAGTCATCACCGAAGAAACAACCGATTTTGTTATGGATTTCAAAACAAGTAGCGCTGAGGGAAATGT
TGATTTTATTAACGTTTTTGACCTTGAGAAAATGGCACAACAGGTCATCCCTAAAGGAGCCTTTGGTTATATTGCTAGTG
GTGCTGGTGATACCTTTACCCTCCATGAAAATATTCGCTCATTTAATCATAAATTAATTGTCCCTCACTCCCTCAAAGGG
GTTGAAAATCCGTCAACAGAGATTACGTTTGATGGTGATTACTTAACCTCTCCTCTTATTTTAGCACCTGTAGCGGCTCA
TAAACTGGCCAATGAACAAGGAGAAGTAGCGTCTGCTAAAGGATTGAAAGAATTTGGAAGTATCTACACCACTAGTTCCT
ACTCAACAACTGACCTGCCAGAAATCAGTGCTGCCTTAGGTGGGACACCCCATTGGTTTCAATTTTATTACAGTAAAGAC
GATGGCATTAATCGTAATATTATGGATCGTGTCAAAGCACAAGGTTGCAAAGCTATCGTTTTGACAGCAGATGCAACAGT
TGGAGGAAACCGCGAAGTTGATAGGCGAAACGGTTTTGTTTTTCCGGTCGGTATGCCAATTGTTCAAGAATATTTACCTG
ATGGTGCAGGTAAAACAATGGATTATGTCTATAAATCAGCTAAACAAGCGCTTACCTCAAAAGATATTGAATATATCGCA
ACCTACTCAGGCCTGCCTGTTTATGTCAAAGGACCACAGTGTGCTGAGGATACCTTGCGGGCATTGGACGCAGGAGCATC
AGGTATCTGGGTAACCAATCATGGTGGCCGTCAATTAGATGGTGGTCCAGCTGCTTTTGATTCCTTACAAGAAGTCGCTG
AAGCAGTTGATCACAAAGTTCCTATTGTCTTTGACTCAGGTATAAGATGGGGTCAACATATTTTCAAAGCTCTTGCTTCA
GGTGCTGACCTTGTTGCCTTGGGGCGTCCAGCGATTTACGGCTTGGCTATGGGAGGAAGCATTGGTACTCGTCAGGTCTT
TGAAAAGTTAAATGACGAGTTAAAAATGGTCATGCAACTGGCTGGGACACAAACTATCCAAGATGTTAAAGCCTTTAACC
TTCGCCATAATCCGTATGATTCATCGATTCCATTTGATCAAAATGCGCTACGCTTAGATTAG

Upstream 100 bases:

>100_bases
ATCAGTGAGCATATCGTTTGACTGACAGTTAAGAGTAGGATTACTATAATAGAAGCATAAATGTAAGCGCTAACAAAATA
AAAAATATAGGAGAATGTCA

Downstream 100 bases:

>100_bases
AACAGACAAAAACCTCTGCTATCATGTCACAAATAGTAGAGGTTTTACACTGCAAAAAGAGGTATGAAAATCCAATAACA
TAGTTACAAGGCTTTCGTTT

Product: L-lactate oxidase

Products: NA

Alternate protein names: Lactate oxidase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKG
VENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYIA
TYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRWGQHIFKALAS
GADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD

Sequences:

>Translated_393_residues
MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKG
VENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYIA
TYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRWGQHIFKALAS
GADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
>Mature_392_residues
AQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGV
ENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKDD
GINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYIAT
YSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRWGQHIFKALASG
ADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD

Specific function: Unknown

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=361, Percent_Identity=32.6869806094183, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI54234014, Length=343, Percent_Identity=31.4868804664723, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI7705393, Length=343, Percent_Identity=31.4868804664723, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1790033, Length=364, Percent_Identity=31.5934065934066, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI193208036, Length=354, Percent_Identity=37.2881355932203, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI32566217, Length=263, Percent_Identity=36.8821292775665, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6323587, Length=358, Percent_Identity=31.8435754189944, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI78707188, Length=357, Percent_Identity=35.2941176470588, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI281363140, Length=357, Percent_Identity=35.2941176470588, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI78707190, Length=357, Percent_Identity=35.2941176470588, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.13.12.4 [H]

Molecular weight: Translated: 42620; Mature: 42488

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFT
CCCCCEEECCCHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCCCEEE
LHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAK
EHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHHHH
GLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKDDGINRNIMDRVKAQGCKAIV
HHHHHCCEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
LTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYIA
EEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDHKV
ECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
PIVFDSGIRWGQHIFKALASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQL
CEEEECCCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
HCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
AQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIPKGAFGYIASGAGDTFT
CCCCEEECCCHHHEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCCCEEE
LHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILAPVAAHKLANEQGEVASAK
EHHHHHHCCCEEEECCCCCCCCCCCCEEEECCCHHCCCHHHHHHHHHHHCCCCCCHHHHH
GLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKDDGINRNIMDRVKAQGCKAIV
HHHHHCCEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
LTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGKTMDYVYKSAKQALTSKDIEYIA
EEECCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGGRQLDGGPAAFDSLQEVAEAVDHKV
ECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
PIVFDSGIRWGQHIFKALASGADLVALGRPAIYGLAMGGSIGTRQVFEKLNDELKMVMQL
CEEEECCCHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AGTQTIQDVKAFNLRHNPYDSSIPFDQNALRLD
HCCHHHHHHHHHCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2324094; 3571231 [H]