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The map label for this gene is yusI [H]

Identifier: 21909831

GI number: 21909831

Start: 331665

End: 332021

Strand: Direct

Name: yusI [H]

Synonym: SpyM3_0295

Alternate gene names: 21909831

Gene position: 331665-332021 (Clockwise)

Preceding gene: 21909829

Following gene: 21909833

Centisome position: 17.45

GC content: 40.34

Gene sequence:

>357_bases
ATGATTACGATTTACCAATACCCAAAATGTAGCACCTGCCAAAGAGCCATGGCTGAATTAAAGCAACTGGTTAGTGACTT
TGAAGCCATTGACATCAAAGCTAATCCACCCAAAGCTCAGGACCTCAAACATTGGATGGAAACATCTGGTTATACCATCA
AAAATTTCTTTAATACCAGTGGTAACAGCTACCGCGAATTGGGACTTAAAGATAAAATAGACCAGCTTAGCCTTGATAAA
GCTGCTGAACTCTTAGCCACAGATGGTATGCTGATTAAACGGCCTATTCTGATTAAGGACGGTAAAGTCCTACAAGTTGG
CTATCGCAAGCCATATCAAGAGTTGGATTTAGGATAA

Upstream 100 bases:

>100_bases
AAGACAAAGCTTAGCATTTTAAAAAAGCCCCCTTTTTTTGTGAAATGATGACCAAAAACGGGCGCCTCACGCCTAAAACA
GTTACCCTAAAGGAGAACAA

Downstream 100 bases:

>100_bases
TGCGACAAAGTCTCAAAAAAGGACAAGGCAACATTATAACGCCTTGTCCTTTTTTGGGATCTGTTTTTAGACATTAGAGA
TCAATATCTAGTAAAATTGG

Product: putative arsenate reductase

Products: arsenite; oxidized glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKHWMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDK
AAELLATDGMLIKRPILIKDGKVLQVGYRKPYQELDLG

Sequences:

>Translated_118_residues
MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKHWMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDK
AAELLATDGMLIKRPILIKDGKVLQVGYRKPYQELDLG
>Mature_118_residues
MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKHWMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDK
AAELLATDGMLIKRPILIKDGKVLQVGYRKPYQELDLG

Specific function: Reduction Of Arsenate [As(V)] To Arsenite [As(III)]. This Protein Expands The Substrate Specificity Of Arsab Pump Which Can Extrude Arsenite And Antimonite To Allow For Arsenate Pumping And Resistance (By Similarity). [C]

COG id: COG1393

COG function: function code P; Arsenate reductase and related proteins, glutaredoxin family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsC family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006660
- InterPro:   IPR012336
- InterPro:   IPR012335
- InterPro:   IPR006504 [H]

Pfam domain/function: PF03960 ArsC [H]

EC number: 1.20.4.1 [C]

Molecular weight: Translated: 13431; Mature: 13431

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKHWMETSGYTIKNFFNTS
CEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
GNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGKVLQVGYRKPYQELDLG
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCEEEECCCCCHHHCCCC
>Mature Secondary Structure
MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKHWMETSGYTIKNFFNTS
CEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
GNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGKVLQVGYRKPYQELDLG
CCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arsenate; reduced glutathione [C]

Specific reaction: arsenate + reduced glutathione = arsenite + oxidized glutathione [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]