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The map label for this gene is braB [H]

Identifier: 21909772

GI number: 21909772

Start: 275216

End: 276586

Strand: Reverse

Name: braB [H]

Synonym: SpyM3_0236

Alternate gene names: 21909772

Gene position: 276586-275216 (Counterclockwise)

Preceding gene: 21909774

Following gene: 21909750

Centisome position: 14.55

GC content: 36.98

Gene sequence:

>1371_bases
ATGAAACAAAAAAATGTATATATCGTCATTGGATTCATGCTATTTGCACTATTTTTTGGAGCAGCAAACCTCATTTACCC
AGCTTTTTTAGGCATCTACTCAGGTCATCAGATTCTATGGTCTATCATTGGTTTTTGTTTAACTGGTGTCTCCTTGCCTT
TACTCGGTGTCATTGCTGTTGCTAAATCTGGATCAGGCGATGTTGAAAGTTTGGCACGCCCCATCTCTAAATGGTATGCA
ATCTTCTATTCTTCCATTTTGTATTTATCTATTGGTCCATTTTTTGCTATTCCAAGAACAGGAGCCACTTCTTTTTCAGT
CGGTATCGCTCCTATCTTAGGAGATAATGCAACCAATAAAGCTATTTATGCTATACTATTTTTTGGTCTGTCCTACTTCC
TTGCTATCAAACCTAGTAAGCTAGCTGAAAATATCGGAAAATTTTTAACGCCAACGTTGTTAGTTGTTATTTCTATTTTG
GTTATCGCGTCCTTTGTCCATCCTGCTGGAAATTACGGTGATACTTTTAACGCTGGGGTCGGTGTTAATAATGCCTTTAA
AGATTTTCCTTTTATAGCAGGATTAATTCAAGGTTATGGCACTATGGATGCACTAGCTTCTCTTGTTTTTGCTATTTTAG
TCATTGAGGCTACCAAACAATTTGGCGCTAAGACGGACAAAGAAGTGACCAAAATAACACTTATTTCTGGGGCTATTGCC
ATTTTGCTATTAGCACTTGTCTATATCTTTGTCGGTCGTATTGGAGCAACATCACAATCATTATTTCCTTTTAGTGATGG
CAGCTTTACCCTTCATGGTAATCCAGTTAATGGCGGTCAAATCTTAAGTCATGCTTCTCGTTTTTACCTAGGTGGCATCG
GACAAGCATTTCTAGCTGTTGTGATTTTCCTGGCCTGTCTAACCACTTCAACAGGCTTAATCACGTCAAGTGCTGAATAC
TTCCATAAATTAGTGCCTGCTTTATCTCATATTGCTTGGGCAACTATCTTTACTTTACTATCAGCTTTCTTTTATTTTGG
TGGTTTATCAGTTATTATCAACTGGTCAGCTCCTGTTTTATTCCTTTTATACCCATTAACAGTCGATTTAATTTTCCTTG
TTTTGGCACAAAAATGCTTCAATAATGATCCTATTGTCTATCGAACTACAATTGGTCTAACCTTTATTCCTGCCATATTT
GATGCACTCCTAACACTATCACAAATGACTGGATTATTTCATTTACCAGAAGCCGTTGTAACTTTTTTCCAAAAAACTGT
TCCACTAGGGCAATTCTCAATGGGATGGATTATCTTTGCTGCTATTGGTTTTTTAATAGGGCTTATACTAAGTAAAACGA
AGAAAAGCTAA

Upstream 100 bases:

>100_bases
GTAATCTTAGATATAATAACCTTTGTTGTTATTTTGACACATAATATAATTGAAATTGTTAGAATCTTCGGATTAGTATC
AATAGAAAGACTATTTATAA

Downstream 100 bases:

>100_bases
TGATATCATCTAAACAACAACACCCTAAGTAAAAACTTAGGGTGTTGTTTCGTTTATTTATGACTAAACCTTCTAGTCAA
GCTATCACCAATAAATTGGA

Product: putative branched-chain amino acid transport protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYA
IFYSSILYLSIGPFFAIPRTGATSFSVGIAPILGDNATNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
VIASFVHPAGNYGDTFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVTKITLISGAIA
ILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEY
FHKLVPALSHIAWATIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIF
DALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS

Sequences:

>Translated_456_residues
MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYA
IFYSSILYLSIGPFFAIPRTGATSFSVGIAPILGDNATNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
VIASFVHPAGNYGDTFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVTKITLISGAIA
ILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEY
FHKLVPALSHIAWATIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIF
DALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS
>Mature_456_residues
MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYA
IFYSSILYLSIGPFFAIPRTGATSFSVGIAPILGDNATNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
VIASFVHPAGNYGDTFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVTKITLISGAIA
ILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEY
FHKLVPALSHIAWATIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIF
DALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=452, Percent_Identity=32.3008849557522, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49023; Mature: 49023

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLGVIAV
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGATSFSVGIAPILGDNATNK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCCCCHH
AIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISILVIASFVHPAGNYGDTFNAGV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVTKITLISGAIA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRFYLGGIGQAFLAV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHH
VIFLACLTTSTGLITSSAEYFHKLVPALSHIAWATIFTLLSAFFYFGGLSVIINWSAPVL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHH
FLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIFDALLTLSQMTGLFHLPEAVV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIGFCLTGVSLPLLGVIAV
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGATSFSVGIAPILGDNATNK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCCCCHH
AIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISILVIASFVHPAGNYGDTFNAGV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEATKQFGAKTDKEVTKITLISGAIA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPVNGGQILSHASRFYLGGIGQAFLAV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHH
VIFLACLTTSTGLITSSAEYFHKLVPALSHIAWATIFTLLSAFFYFGGLSVIINWSAPVL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHH
FLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIGLTFIPAIFDALLTLSQMTGLFHLPEAVV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFFQKTVPLGQFSMGWIIFAAIGFLIGLILSKTKKS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]