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The map label for this gene is dacA [H]

Identifier: 21909750

GI number: 21909750

Start: 247173

End: 248405

Strand: Reverse

Name: dacA [H]

Synonym: SpyM3_0214

Alternate gene names: 21909750

Gene position: 248405-247173 (Counterclockwise)

Preceding gene: 21909772

Following gene: 21909749

Centisome position: 13.07

GC content: 35.69

Gene sequence:

>1233_bases
ATGATCAAACGATTAATTTCCCTAGTGGTCATCGCCTTATTTTTTGCAGCAAGCACTGTTAGCGGTGAAGAGTATTCGGT
AACTGCTAAGCATGCGATTGCCGTTGACCTTGAAAGTGGCAAAGTTTTATACGAAAAAGATGCTAAAGAGGTTGTCCCAG
TCGCCTCAGTCAGTAAGCTCTTGACAACCTATCTGGTTTACAAAGAAGTTTCTAAGGGCAAGCTAAATTGGGATAGTCCT
GTAACTATTTCTAACTACCCTTATGAACTCACTACAAACTATACTATTAGTAACGTTCCTCTTGATAAGAGAAAATATAC
CGTTAAAGAACTTTTAAGTGCGTTAGTTGTTAATAACGCCAATAGCCCCGCTATTGCTTTAGCTGAAAAAATAGGCGGAA
CCGAACCCAAATTTGTTGACAAAATGAAAAAACAATTAAGACAATGGGGCATTTCCGATGCAAAGGTCGTCAATTCAACT
GGCTTAACTAACCATTTTTTAGGAGCTAATACTTATCCTAATACAGAACCAGATGATGAAAATTGTTTTTGCGCCACTGA
TTTAGCTATTATTGCCAGGCATCTCTTATTAGAATTTCCAGAAGTACTGAAATTATCTAGCAAATCCTCCACTATTTTTG
CTGGACAAACCATTTACAGTTATAATTACATGCTTAAAGGCATGCCTTGTTATCGAGAAGGCGTGGATGGTCTTTTTGTT
GGTTATTCTAAAAAAGCCGGTGCTTCTTTTGTAGCTACTAGTGTCGAAAATCAAATGAGGGTTATTACAGTAGTTTTAAA
TGCTGATCAAAGCCACGAGGATGATTTAGCTATATTTAAAACAACCAATCAATTGTTGCAGTACCTTTTAATTAATTTTC
AAAAAGTCCAGTTAATTGAAAATAATAAACCAGTAAAAACGTTATCTGTCTTAGACAGTCCTGAAAAAACTGTCAAACTT
GTAGCCCAAAATAGTTTATTTTTTATCAAACCAATACATACAAAGACCAAAAATACCGTCCATATTACTAAGAAATCATC
CACAATGATCGCACCTCTATCAAAGGGACAAGTCTTAGGTAGAGCAACCCTTCAAGATAAACATCTTATTGGACAAGGTT
ATCTGGATACTCCTCCTTCTATCAATCTTATCCTTCAAAAAAACATTTCTAAAAGTTTCTTTTTAAAGGTCTGGTGGAAC
CGTTTTGTGAGGTATGTCAATACCTCTTTATAG

Upstream 100 bases:

>100_bases
AGCGTTTTATTACATCACTACTCTTGTGAGGTAAGTCATTACCTTAGACAAAAATATGGTATAATTAAAAAAATAATACT
TGAGTAAGGATAATCTATAA

Downstream 100 bases:

>100_bases
AATAACACAATGCTTTTTGTATGACATTGACTGACAGAGTACTTCTGCAGTCTTTTTCATTTTTATTTAAGCAAACTAGG
CTAATCCTAACAATTTTTCA

Product: putative D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLYEKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSP
VTISNYPYELTTNYTISNVPLDKRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTIYSYNYMLKGMPCYREGVDGLFV
GYSKKAGASFVATSVENQMRVITVVLNADQSHEDDLAIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKL
VAQNSLFFIKPIHTKTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKSFFLKVWWN
RFVRYVNTSL

Sequences:

>Translated_410_residues
MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLYEKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSP
VTISNYPYELTTNYTISNVPLDKRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTIYSYNYMLKGMPCYREGVDGLFV
GYSKKAGASFVATSVENQMRVITVVLNADQSHEDDLAIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKL
VAQNSLFFIKPIHTKTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKSFFLKVWWN
RFVRYVNTSL
>Mature_410_residues
MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLYEKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSP
VTISNYPYELTTNYTISNVPLDKRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTIYSYNYMLKGMPCYREGVDGLFV
GYSKKAGASFVATSVENQMRVITVVLNADQSHEDDLAIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKL
VAQNSLFFIKPIHTKTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKSFFLKVWWN
RFVRYVNTSL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=383, Percent_Identity=24.2819843342037, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787062, Length=409, Percent_Identity=25.6723716381418, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786851, Length=377, Percent_Identity=24.9336870026525, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45690; Mature: 45690

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLYEKDAKEVVPVASVSKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCEEEECCCHHHCCHHHHHHH
LTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLDKRKYTVKELLSALVVNNA
HHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHCCC
NSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNSTGLTNHFLGANTYPNTEPDDE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCCCCCCCCCCCC
NCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTIYSYNYMLKGMPCYREGVDGLFV
CCEEEHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCEEEEEEHEECCCCHHHHCCCEEEE
GYSKKAGASFVATSVENQMRVITVVLNADQSHEDDLAIFKTTNQLLQYLLINFQKVQLIE
ECCCCCCCCEEEHHHCCCEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCEEEEEEC
NNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHTKTKNTVHITKKSSTMIAPLSKGQVLG
CCCCEEEEEECCCCHHHEEEEECCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCEEE
RATLQDKHLIGQGYLDTPPSINLILQKNISKSFFLKVWWNRFVRYVNTSL
EECCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLYEKDAKEVVPVASVSKL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCEEEECCCHHHCCHHHHHHH
LTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLDKRKYTVKELLSALVVNNA
HHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHCCC
NSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNSTGLTNHFLGANTYPNTEPDDE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCCCCCCCCCCCC
NCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTIYSYNYMLKGMPCYREGVDGLFV
CCEEEHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCEEEEEEHEECCCCHHHHCCCEEEE
GYSKKAGASFVATSVENQMRVITVVLNADQSHEDDLAIFKTTNQLLQYLLINFQKVQLIE
ECCCCCCCCEEEHHHCCCEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCEEEEEEC
NNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHTKTKNTVHITKKSSTMIAPLSKGQVLG
CCCCEEEEEECCCCHHHEEEEECCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCEEE
RATLQDKHLIGQGYLDTPPSINLILQKNISKSFFLKVWWNRFVRYVNTSL
EECCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]