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The map label for this gene is ybfL [H]

Identifier: 21909757

GI number: 21909757

Start: 262681

End: 263097

Strand: Direct

Name: ybfL [H]

Synonym: SpyM3_0221

Alternate gene names: 21909757

Gene position: 262681-263097 (Clockwise)

Preceding gene: 21909756

Following gene: 21909758

Centisome position: 13.82

GC content: 38.13

Gene sequence:

>417_bases
ATGATTGATTTTATTATTTCTATTGATGATTGCGCAGTTGAATTGGATAGTCGTCAATCTTGGAAAATTCGCTCCCCCTT
ATCAACCATTCTATTTCTTGTCTTCGTTTGTCAGTTAGCTGGCATTGAAACCTGGAAGGAGATGGAAGATTTTATTGAAA
TGAATGAACCATTGTTTGCGACCTACGTTGATTTGAGTGAAGGTTGTCCGTCTCATGATACCTTAGAGCGTGTGATTAGT
CTTGTTAATTCAGAGCGTTTAAAAGAGCTTAAAGTTCAATTTGAGCAATCATTGACAAGCTTAGATGCCGTTCATCAACT
GATTTCAGTGGAGGGTAAAACGATTCGAGACAATCGAGGTAAAAATCAGAAGCCTGTTCATATTGTAACGGCTTATGATG
GGAGGTCATCATCTTAG

Upstream 100 bases:

>100_bases
ACTATCACGCCAAACGCATGCAGAAACTTTTGTTTTTAGAAAACTAGATTCATAATGACAAAATCAATCAAAGTAGTACA
CTATAGATGAGGTGACTACG

Downstream 100 bases:

>100_bases
TTTGGGACAGGTAGCGGTTGAGGAGAAAAGTAATGAAATTGTTGCCATTCCTCAGTTATTGCGGACAATTGATATCCGTA
AAAGCATTGTAACGATAGAC

Product: IS1548 transposase

Products: NA

Alternate protein names: H repeat-associated protein in rhsC-phrB intergenic region; ORF-H2 [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSERLKELKVQFEQSLTSLDAVHQLISVEGKTIRDNRGKNQKPVHIVTAYDGRSSS

Sequences:

>Translated_138_residues
MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSERLKELKVQFEQSLTSLDAVHQLISVEGKTIRDNRGKNQKPVHIVTAYDGRSSS
>Mature_138_residues
MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVIS
LVNSERLKELKVQFEQSLTSLDAVHQLISVEGKTIRDNRGKNQKPVHIVTAYDGRSSS

Specific function: Unknown

COG id: COG5433

COG function: function code L; Transposase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787733, Length=119, Percent_Identity=31.9327731092437, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1789896, Length=119, Percent_Identity=31.9327731092437, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1786924, Length=119, Percent_Identity=31.0924369747899, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 15706; Mature: 15706

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFA
CCEEEEECCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEE
TYVDLSEGCPSHDTLERVISLVNSERLKELKVQFEQSLTSLDAVHQLISVEGKTIRDNRG
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
KNQKPVHIVTAYDGRSSS
CCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MIDFIISIDDCAVELDSRQSWKIRSPLSTILFLVFVCQLAGIETWKEMEDFIEMNEPLFA
CCEEEEECCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEE
TYVDLSEGCPSHDTLERVISLVNSERLKELKVQFEQSLTSLDAVHQLISVEGKTIRDNRG
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
KNQKPVHIVTAYDGRSSS
CCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8387990; 8905232; 9278503 [H]