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The map label for this gene is pbp1B [H]

Identifier: 21909610

GI number: 21909610

Start: 91788

End: 94088

Strand: Direct

Name: pbp1B [H]

Synonym: SpyM3_0074

Alternate gene names: 21909610

Gene position: 91788-94088 (Clockwise)

Preceding gene: 21909607

Following gene: 21909611

Centisome position: 4.83

GC content: 40.37

Gene sequence:

>2301_bases
ATGGTGAAATGGAACACGAAACAAAAGCGTATAAGTCATCAAAGATTAGGTCTCTTGGATTTGGGGCCGGTGCTATTACG
TACGTTGAGACTACTGTCTAACTTTTTTTATATTGTTATCTTCCTTTTTGGAATGATGGGATTTGGTATGGCATTTGGGT
ATTTGGCTAGTCAGATTGAATCTGTTAAGGTACCAAGTAAAGAAAGTTTAGTCAAACAAGTTGAATCATTAACGATGATT
TCGCAAATGAACTATTCTGATAATAGTTTAATTTCTACTTTAGATACGGATTTACTTCGAACACCAGTAGCTAATGATGC
GATTTCAGAGAATATCAAAAAAGCTATTGTATCAACAGAAGACGAACATTTTCAAGAACATAAAGGTGTCGTGCCAAAAG
CTGTTTTTCGGGCAACATTGGCTTCTGTATTGGGATTTGGAGAAGCTAGTGGAGGTTCGACCTTAACACAGCAATTGGTC
AAACAACAAGTTTTGGGAGATGATCCCACATTTAAGCGCAAGTCTAAGGAGATCGTTTACGCTCTTGCCTTAGAGCGTTA
TATGTCCAAAGACAATATCTTATGTGATTATCTTAATGTTTCACCTTTTGGTCGTAACAACAAGGGCCAAAATATTGCTG
GTGTTGAAGAAGCTGCGCGTGGCATTTTTGGCGTTTCTGCCAAAGATTTAACGGTGCCACAGGCAGCATTTTTGGCGGGG
CTTCCGCAGAGTCCTATTGTTTACTCTCCTTATTTGTCAACGGGACAACTGAAGTCAGAAAAGGACATGGCTTATGGCAT
CAAGCGTCAGCAAAATGTTCTCTATAACATGTACCGTACAGGTGTTCTGTCTAAAAAAGAATACGAGGACTATAAGGCTT
ATCCGATTCAAAAGGATTTTATTCAACCGGGAAGTGCAATAGTAAATAATCACGATTACCTTTATTACACGGTGTTAGCG
GATGCTAAAAAAGCCATGTATAGCTATTTGATTAAGCGAGATAAGGTGTCTAGTCGTGATTTGAAAAATGACGAGACTAA
GGCTGCTTATGAAGAGAGAGCCTTAACAGAATTGCAACAGGGTGGCTATACCATCACCACAACCATTAATAAGCCTATTT
ACAATGCGATGCAGACAGCGGCAGCTCAGTTTGGTGGCTTGTTAGATGATGGCACTGGTACAGTTCAAATGGGAAATGTC
TTGACAGACAATGCGACTGGTGCTGTGTTAGGTTTTGTTGGTGGTAGAGATTATGCTCTGAATCAAAATAATCATGCTTT
CAATACAGTTAGATCGCCAGGTTCTAGCATTAAACCGATAATCGCTTATGGTCCTGCTATTGATCAAGGTTTAATGGGGA
GTGCTAGCGTTTTGTCTAATTACCCAACAACTTACTCGAGTGGCCAAAAAATCATGCATGCTGATAGTGAAGGAACAGCC
ATGATGCCACTTCAAGAGGCCCTAAATACTTCTTGGAATATCCCAGCTTTTTGGACACAGAAATTACTGCGTGAAAAAGG
GGTCGATGTCGAAAATTATATGACAAAAATGGGTTATAAGATTGCAGACTACTCGATTGAAAGTTTACCTCTAGGGGGCG
GTATTGAAGTCTCGGTTGCTCAACAAACCAATGCTTACCAAATGCTTTCAAACAATGGCTTATATCAAAAGCAATATATT
GTAGATAAGATTACTGCTAGCGATGGTACAGTCGTTTACAAACATGAAAATAAGCCAATTCGTATTTTTTCTGCAGCAAC
AGCTACGATTTTACAAGAATTGTTGAGAGGTCCGATTACTTCAGGCGCTACGACTACTTTCAAGAACCGTTTGGCGGCTA
TTAATCCGTGGCTTGCTAATGCTGATTGGATTGGTAAGACCGGAACAACTGAGAATTATACGGATGTTTGGCTAGTCCTG
TCTACTCCAAAAGTTACCTTAGGCGGTTGGGCAGGACATGATGACAATACCTCATTAGCGCCATTAACAGGATATAACAA
TAATTCTAATTACCTTGCCTATTTAGCTAATGCCATTAATCAGGCCGATCCCAATGTTATTGGAGTAGGGCAACGTTTCA
ACTTAGATCCAGGAGTCATTAAGGCGAATGTCTTGAAGTCAACAGGTTTACAACCAGGAACTGTTAATGTCAATGGACAT
ACTTTTTCTGTTGGTGGAGAAATGACCACCAGTCTATGGTCCCAAAAAGGACCGGGGGCTATGACTTACCGATTTGCTAT
TGGTGGCACGGATGCCGATTATCAAAAAGCCTGGGGGAACTTCGGGTTCAGAAAAAATTAG

Upstream 100 bases:

>100_bases
CTTTCTCCATTTTCATTATAGTTTCGAATCATTATATCAAAAAACGCTACTGCTATGCAAGCTTTATCAAAGTTTGGTAT
AATGATAAATGAGGTATTTT

Downstream 100 bases:

>100_bases
GTTGCCATTTATCGATAAAAAGGGTATAATAGTAATAACTATTTGTCGTCCAATCTAGCTGAAATATTGTCCAGTTAGGA
AGAACAGCAGTTAAATCACA

Product: putative penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 766; Mature: 766

Protein sequence:

>766_residues
MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMI
SQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLTQQLV
KQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAAFLAG
LPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLA
DAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNV
LTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTA
MMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYI
VDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVL
STPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGH
TFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN

Sequences:

>Translated_766_residues
MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMI
SQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLTQQLV
KQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAAFLAG
LPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLA
DAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNV
LTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTA
MMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYI
VDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVL
STPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGH
TFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
>Mature_766_residues
MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMI
SQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLTQQLV
KQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAAFLAG
LPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLA
DAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNV
LTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTA
MMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYI
VDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVL
STPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGH
TFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=616, Percent_Identity=25.8116883116883, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87082258, Length=349, Percent_Identity=28.6532951289398, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786343, Length=577, Percent_Identity=25.1299826689775, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI1789601, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 83903; Mature: 83903

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIE
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTE
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLTQQLVKQQVLGDDPTFKRKSKEIVY
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAAFLAG
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHC
LPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLYNMYRTGVLSKKEYEDYKAYPIQKDF
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHH
IQPGSAIVNNHDYLYYTVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQ
CCCCCCEECCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
GGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNVLTDNATGAVLGFVGGRDYAL
CCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCEEECCEEECCCCCEEEEEECCCCEEE
NQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTA
CCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCE
MMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVA
ECCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHCCCCCCCEEEEEE
QQTNAYQMLSNNGLYQKQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPIT
CCCCHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCC
SGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCC
PLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGH
EEECCCCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCEEEHHHHHCCCCCCEEEECCE
TFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
EEECCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIE
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTE
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLTQQLVKQQVLGDDPTFKRKSKEIVY
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAAFLAG
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHC
LPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLYNMYRTGVLSKKEYEDYKAYPIQKDF
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHH
IQPGSAIVNNHDYLYYTVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQ
CCCCCCEECCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
GGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNVLTDNATGAVLGFVGGRDYAL
CCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCEEECCEEECCCCCEEEEEECCCCEEE
NQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTA
CCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCE
MMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVA
ECCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHCCCCCCCEEEEEE
QQTNAYQMLSNNGLYQKQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPIT
CCCCHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCC
SGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCC
PLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGH
EEECCCCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCEEEHHHHHCCCCCCEEEECCE
TFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
EEECCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]