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The map label for this gene is glyS [H]

Identifier: 30410816

GI number: 30410816

Start: 140651

End: 142789

Strand: Reverse

Name: glyS [H]

Synonym: BUsg127

Alternate gene names: 30410816

Gene position: 142789-140651 (Counterclockwise)

Preceding gene: 21672418

Following gene: 21672405

Centisome position: 22.26

GC content: 22.07

Gene sequence:

>2139_bases
TTGCACAAGAATATTTATATTCAAGAAAAAAATAAAATTTTTATTATAATTAACGAGAAAAAAATGATAAAAAAAACATT
TTTAGTCGAAATAGGAACTGAAGAACTACCTTCTAACATCCTTAAAAAAATTATTTTAGTTTTTTACAAAAATTTTGTAG
ATGAACTAAGTTTTAATAAAATTTTATATAAAAACATCAATTATTTTTCTACTCCAAGAAGATTAGCATTACAAATTATA
GAACTTGACACTTCAGAAAAAATAAATGAAAAAATAAAAAAAGGTCCAGCAATAAAACATGCTTTTGATGAAAACGGTAA
TCCAACAAAAGCAGCTTACTATTGGGCTAAGAGTTGTAAAATAAATTTAAATCAAGCTGAAAGATTGAAAACTAAAACAG
GAGAATGGATCATACATCGTATACAAGAAAAAAAAGAAAAAATTGAATTGTTATTTCCTAAAATAATAGAAAAAATACTT
AAACAAATTAATTTGAAAAACACGATGAGATGGGAAACTACTAATCTTAGATTTATTCGTCCTATCCGTAATATTGTAAT
GCTATTAGATGAAAAAATTATTAAAAGCGAAGTATTTAATGTACCTTCTAATAATTTTCTTTATCACCATATTTCTTGTA
AAGAAGAAAAAATATACATTAAAAATGCAATAGAATATCCTGCAGTTCTTTTTAAAAAAAATAAAATAATAGCTAATTAT
GAAACTCGTAAAGAAAAAATAAAATATGAAGCAAAAAAAATAGCAAAAAAAGTTGATGGAATTATAAAAACGAATCCTTT
TCTACTTGAAGAAGTTACTTCTTTAGTAGAATCACCTCAAGCACTTTTAGCACGTTTTAAAAAAGATTATATTAATTACA
TACCTAAAAAAATACTTATTCATACTATAGAAAAACAACAAAGATGCTTTTCAATATATAGTAATAATTCAAAAAAAGAA
ATTTTACCATATTTTATTTTTATTTCTAATATAAAATCTAAAAAAAATAAAGAAATTATTTTAGGAAATGAAAAAGTTAT
GCATGCACGTTTATCAGATGCTATGTTTTTTTTAAAACAAGATAGAAAAAGGAAATTAGAAAATTATCTCCCATTTTTAA
AAAAAGTTTCATTTTATAATAATTTAGGTACATTATATGAAAAAACATTACGTTTAAAATTACTTATAGAATCTATTTCG
TGTTATACCAATACAATTAATAAAAATGACTTAATCAGATCAGCTGTTTTATCAAAATGTGATCTCATAACTCAAATGGT
AGATGAATTTCCAGAATTACAAGGCACTATAGGAATGTATTACGCTTTAGAAAACAAAGAGAATAAAGAAGTCGCTATAG
CTATTAAAGAACAATATTTACCGTCTTTTTCAGGTGATGAATTACCTTCTACCCCGATAGGTTGTCTATTATCTATTTCT
GATAAAATTGATACTTTATCAGGAATGTTCGCGATTGGAGAGACTCCAACACCAGAAAAAGATCCATTTGGATTAAGACG
TGCTGCTTTAGGAATATTACGCATTATTATAACAAAAAAAATACCAATAGATTTAAAAAAAATAATTGAAATCAGCCTAA
AAATTTATACTTTTAAAAAAGTAAACTATTCAATTATATCTAAAAAGATTATAAAATTCTTTATATCACGATTATCGTTT
TTTTATGAAAAAAAAGGATATAGTACTAAAGTCATTAAATCAGTATTATCCTGTAAATTGACAGAACCTTTAGATATCGA
TAAAAGAATTAATGCAATATCTGATCTTAAAAAAATAGAATCAATTATATTAATTGCTAAACGAATAGATAATATAGTAA
AAAACAACCATCAAATAATTTCTAGTGAAATTCATGTTGAACTGATGAAAAAAACAGAAGAAAAAAATTTATTAAAAGAA
ATAAAAATTTTTAATAGTAAAACAAAAGAATTATTTATACAAAAAAAATATAAAGAAATATTAGTAGAAATAAAAAAACT
TGAAAAACCAATAGATAATTTTTTTGATAAAGTACAAATAAATCATTCAAATTTTGAAATTAGACAAAATAGATTATTGC
TTTTAATAAAAATTCAAAAATTTTTTTTAAAAATAGCAAATTTTTCTTACTTATATTAA

Upstream 100 bases:

>100_bases
CAAGCAAGTCATGTATTTAATTTACTAGATGCAAGAAAAGCAATATCTTCTAGTGAACGTCAAAATTATATTTTAAAAAT
TCGAAAATTAGCTAAAAAAA

Downstream 100 bases:

>100_bases
AAAACATAAAAATTTTAATTAAAAATTATTTATAAAGATTCTAAGGTACCTTTAGGAAGAGATGAAACTATAAAATCTTT
TTTTATAAATATTTCTGTGG

Product: glyS: glycyl-tRNA synthetase beta chain

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MHKNIYIQEKNKIFIIINEKKMIKKTFLVEIGTEELPSNILKKIILVFYKNFVDELSFNKILYKNINYFSTPRRLALQII
ELDTSEKINEKIKKGPAIKHAFDENGNPTKAAYYWAKSCKINLNQAERLKTKTGEWIIHRIQEKKEKIELLFPKIIEKIL
KQINLKNTMRWETTNLRFIRPIRNIVMLLDEKIIKSEVFNVPSNNFLYHHISCKEEKIYIKNAIEYPAVLFKKNKIIANY
ETRKEKIKYEAKKIAKKVDGIIKTNPFLLEEVTSLVESPQALLARFKKDYINYIPKKILIHTIEKQQRCFSIYSNNSKKE
ILPYFIFISNIKSKKNKEIILGNEKVMHARLSDAMFFLKQDRKRKLENYLPFLKKVSFYNNLGTLYEKTLRLKLLIESIS
CYTNTINKNDLIRSAVLSKCDLITQMVDEFPELQGTIGMYYALENKENKEVAIAIKEQYLPSFSGDELPSTPIGCLLSIS
DKIDTLSGMFAIGETPTPEKDPFGLRRAALGILRIIITKKIPIDLKKIIEISLKIYTFKKVNYSIISKKIIKFFISRLSF
FYEKKGYSTKVIKSVLSCKLTEPLDIDKRINAISDLKKIESIILIAKRIDNIVKNNHQIISSEIHVELMKKTEEKNLLKE
IKIFNSKTKELFIQKKYKEILVEIKKLEKPIDNFFDKVQINHSNFEIRQNRLLLLIKIQKFFLKIANFSYLY

Sequences:

>Translated_712_residues
MHKNIYIQEKNKIFIIINEKKMIKKTFLVEIGTEELPSNILKKIILVFYKNFVDELSFNKILYKNINYFSTPRRLALQII
ELDTSEKINEKIKKGPAIKHAFDENGNPTKAAYYWAKSCKINLNQAERLKTKTGEWIIHRIQEKKEKIELLFPKIIEKIL
KQINLKNTMRWETTNLRFIRPIRNIVMLLDEKIIKSEVFNVPSNNFLYHHISCKEEKIYIKNAIEYPAVLFKKNKIIANY
ETRKEKIKYEAKKIAKKVDGIIKTNPFLLEEVTSLVESPQALLARFKKDYINYIPKKILIHTIEKQQRCFSIYSNNSKKE
ILPYFIFISNIKSKKNKEIILGNEKVMHARLSDAMFFLKQDRKRKLENYLPFLKKVSFYNNLGTLYEKTLRLKLLIESIS
CYTNTINKNDLIRSAVLSKCDLITQMVDEFPELQGTIGMYYALENKENKEVAIAIKEQYLPSFSGDELPSTPIGCLLSIS
DKIDTLSGMFAIGETPTPEKDPFGLRRAALGILRIIITKKIPIDLKKIIEISLKIYTFKKVNYSIISKKIIKFFISRLSF
FYEKKGYSTKVIKSVLSCKLTEPLDIDKRINAISDLKKIESIILIAKRIDNIVKNNHQIISSEIHVELMKKTEEKNLLKE
IKIFNSKTKELFIQKKYKEILVEIKKLEKPIDNFFDKVQINHSNFEIRQNRLLLLIKIQKFFLKIANFSYLY
>Mature_712_residues
MHKNIYIQEKNKIFIIINEKKMIKKTFLVEIGTEELPSNILKKIILVFYKNFVDELSFNKILYKNINYFSTPRRLALQII
ELDTSEKINEKIKKGPAIKHAFDENGNPTKAAYYWAKSCKINLNQAERLKTKTGEWIIHRIQEKKEKIELLFPKIIEKIL
KQINLKNTMRWETTNLRFIRPIRNIVMLLDEKIIKSEVFNVPSNNFLYHHISCKEEKIYIKNAIEYPAVLFKKNKIIANY
ETRKEKIKYEAKKIAKKVDGIIKTNPFLLEEVTSLVESPQALLARFKKDYINYIPKKILIHTIEKQQRCFSIYSNNSKKE
ILPYFIFISNIKSKKNKEIILGNEKVMHARLSDAMFFLKQDRKRKLENYLPFLKKVSFYNNLGTLYEKTLRLKLLIESIS
CYTNTINKNDLIRSAVLSKCDLITQMVDEFPELQGTIGMYYALENKENKEVAIAIKEQYLPSFSGDELPSTPIGCLLSIS
DKIDTLSGMFAIGETPTPEKDPFGLRRAALGILRIIITKKIPIDLKKIIEISLKIYTFKKVNYSIISKKIIKFFISRLSF
FYEKKGYSTKVIKSVLSCKLTEPLDIDKRINAISDLKKIESIILIAKRIDNIVKNNHQIISSEIHVELMKKTEEKNLLKE
IKIFNSKTKELFIQKKYKEILVEIKKLEKPIDNFFDKVQINHSNFEIRQNRLLLLIKIQKFFLKIANFSYLY

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=693, Percent_Identity=40.1154401154401, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 83698; Mature: 83698

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKNIYIQEKNKIFIIINEKKMIKKTFLVEIGTEELPSNILKKIILVFYKNFVDELSFNK
CCCCEEEEECCEEEEEECCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYKNINYFSTPRRLALQIIELDTSEKINEKIKKGPAIKHAFDENGNPTKAAYYWAKSCK
HHHHCCCCCCCCHHHHEEHEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCE
INLNQAERLKTKTGEWIIHRIQEKKEKIELLFPKIIEKILKQINLKNTMRWETTNLRFIR
EECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
PIRNIVMLLDEKIIKSEVFNVPSNNFLYHHISCKEEKIYIKNAIEYPAVLFKKNKIIANY
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHCCCHHEEECCCEEECC
ETRKEKIKYEAKKIAKKVDGIIKTNPFLLEEVTSLVESPQALLARFKKDYINYIPKKILI
HHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
HTIEKQQRCFSIYSNNSKKEILPYFIFISNIKSKKNKEIILGNEKVMHARLSDAMFFLKQ
HHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
DRKRKLENYLPFLKKVSFYNNLGTLYEKTLRLKLLIESISCYTNTINKNDLIRSAVLSKC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DLITQMVDEFPELQGTIGMYYALENKENKEVAIAIKEQYLPSFSGDELPSTPIGCLLSIS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEHHCCCCCCCCCCCCCCHHEEEECC
DKIDTLSGMFAIGETPTPEKDPFGLRRAALGILRIIITKKIPIDLKKIIEISLKIYTFKK
HHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEC
VNYSIISKKIIKFFISRLSFFYEKKGYSTKVIKSVLSCKLTEPLDIDKRINAISDLKKIE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SIILIAKRIDNIVKNNHQIISSEIHVELMKKTEEKNLLKEIKIFNSKTKELFIQKKYKEI
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVEIKKLEKPIDNFFDKVQINHSNFEIRQNRLLLLIKIQKFFLKIANFSYLY
HHHHHHHHHHHHHHHHHEEECCCCCEEECCCEEEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHKNIYIQEKNKIFIIINEKKMIKKTFLVEIGTEELPSNILKKIILVFYKNFVDELSFNK
CCCCEEEEECCEEEEEECCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYKNINYFSTPRRLALQIIELDTSEKINEKIKKGPAIKHAFDENGNPTKAAYYWAKSCK
HHHHCCCCCCCCHHHHEEHEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCE
INLNQAERLKTKTGEWIIHRIQEKKEKIELLFPKIIEKILKQINLKNTMRWETTNLRFIR
EECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHH
PIRNIVMLLDEKIIKSEVFNVPSNNFLYHHISCKEEKIYIKNAIEYPAVLFKKNKIIANY
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHCCCHHEEECCCEEECC
ETRKEKIKYEAKKIAKKVDGIIKTNPFLLEEVTSLVESPQALLARFKKDYINYIPKKILI
HHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
HTIEKQQRCFSIYSNNSKKEILPYFIFISNIKSKKNKEIILGNEKVMHARLSDAMFFLKQ
HHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
DRKRKLENYLPFLKKVSFYNNLGTLYEKTLRLKLLIESISCYTNTINKNDLIRSAVLSKC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DLITQMVDEFPELQGTIGMYYALENKENKEVAIAIKEQYLPSFSGDELPSTPIGCLLSIS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEHHCCCCCCCCCCCCCCHHEEEECC
DKIDTLSGMFAIGETPTPEKDPFGLRRAALGILRIIITKKIPIDLKKIIEISLKIYTFKK
HHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEC
VNYSIISKKIIKFFISRLSFFYEKKGYSTKVIKSVLSCKLTEPLDIDKRINAISDLKKIE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SIILIAKRIDNIVKNNHQIISSEIHVELMKKTEEKNLLKEIKIFNSKTKELFIQKKYKEI
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVEIKKLEKPIDNFFDKVQINHSNFEIRQNRLLLLIKIQKFFLKIANFSYLY
HHHHHHHHHHHHHHHHHEEECCCCCEEECCCEEEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]