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The map label for this gene is hscB

Identifier: 21672835

GI number: 21672835

Start: 636348

End: 636845

Strand: Direct

Name: hscB

Synonym: BUsg579

Alternate gene names: 21672835

Gene position: 636348-636845 (Clockwise)

Preceding gene: 21672834

Following gene: 21672836

Centisome position: 99.2

GC content: 15.66

Gene sequence:

>498_bases
ATGGATTATTTTGCATTATTTAATTTACCAAAAAAATATATAATTGATAAATTTTTACTTTCTAAAAATTTTTATAAATT
ACAGTTAAAATTTCATCCAGATTTATTTATACATCATTCTGAATCTAAAAAAAGAATAGTTTTACAAAAATCAATTGAAA
TTAATAAAGGATATAAAATTTTACAAGATTCATTAAATAGAGCTATACACTTACTTTTTTTAAATGGTTTTAAGATTAGT
AAAGAAAAAGTTTTATCTGAAGATAATAGTTTTTTAAAAAAATATTTTTTTTTATATGAAGAATTAGATTTTTTGTTAAA
AAATAACTGTGATGAAGTTCAAATCGATATTTTTTTTAAAAAAATAAGAAATAAAATAGACAATTATGAAAAAATAATTG
AAATTAAATTTAATGAAAAAAAATGGGATGATATTATTAAATTAATAACTAAACTTCTTTTTTTTAAAAAAATACAAACT
CGTTTAAAACAGTCATAA

Upstream 100 bases:

>100_bases
AATTTTTATTTTTTTAGTTAAAATGTTGAAAGAGATTATCCTTTCAACATTTAATTATGAATTTTAGTATTTTTTAAATA
TATTTTGAATTAGGTGACTT

Downstream 100 bases:

>100_bases
TCACTTATTCAATAAATTGGGATTTTATGCATGGTTTTTTTGAAAAATAAAGTTAAAAAAAAATTAATTTTAGGTATTGA
TTTTGGAACTACTTATTCTC

Product: chaperone protein HscB

Products: NA

Alternate protein names: Hsc20

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MDYFALFNLPKKYIIDKFLLSKNFYKLQLKFHPDLFIHHSESKKRIVLQKSIEINKGYKILQDSLNRAIHLLFLNGFKIS
KEKVLSEDNSFLKKYFFLYEELDFLLKNNCDEVQIDIFFKKIRNKIDNYEKIIEIKFNEKKWDDIIKLITKLLFFKKIQT
RLKQS

Sequences:

>Translated_165_residues
MDYFALFNLPKKYIIDKFLLSKNFYKLQLKFHPDLFIHHSESKKRIVLQKSIEINKGYKILQDSLNRAIHLLFLNGFKIS
KEKVLSEDNSFLKKYFFLYEELDFLLKNNCDEVQIDIFFKKIRNKIDNYEKIIEIKFNEKKWDDIIKLITKLLFFKKIQT
RLKQS
>Mature_165_residues
MDYFALFNLPKKYIIDKFLLSKNFYKLQLKFHPDLFIHHSESKKRIVLQKSIEINKGYKILQDSLNRAIHLLFLNGFKIS
KEKVLSEDNSFLKKYFFLYEELDFLLKNNCDEVQIDIFFKKIRNKIDNYEKIIEIKFNEKKWDDIIKLITKLLFFKKIQT
RLKQS

Specific function: Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward hscA

COG id: COG1076

COG function: function code O; DnaJ-domain-containing proteins 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain

Homologues:

Organism=Escherichia coli, GI1788876, Length=164, Percent_Identity=32.9268292682927, Blast_Score=106, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSCB_BUCAP (O51884)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660902.1
- ProteinModelPortal:   O51884
- SMR:   O51884
- EnsemblBacteria:   EBBUCT00000000347
- GeneID:   1005619
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg579
- GeneTree:   EBGT00050000007802
- HOGENOM:   HBG366228
- OMA:   MNYFKLF
- ProtClustDB:   CLSK316589
- BioCyc:   BAPH198804:BUSG579-MONOMER
- HAMAP:   MF_00682
- InterPro:   IPR001623
- InterPro:   IPR009073
- InterPro:   IPR004640
- Gene3D:   G3DSA:1.10.287.110
- Gene3D:   G3DSA:1.20.1280.20
- SMART:   SM00271
- TIGRFAMs:   TIGR00714

Pfam domain/function: PF00226 DnaJ; PF07743 HSCB_C; SSF46565 DnaJ_N; SSF47144 HSC20_C

EC number: NA

Molecular weight: Translated: 20154; Mature: 20154

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00636 DNAJ_1; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFALFNLPKKYIIDKFLLSKNFYKLQLKFHPDLFIHHSESKKRIVLQKSIEINKGYKI
CCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCHHH
LQDSLNRAIHLLFLNGFKISKEKVLSEDNSFLKKYFFLYEELDFLLKNNCDEVQIDIFFK
HHHHHHHHEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
KIRNKIDNYEKIIEIKFNEKKWDDIIKLITKLLFFKKIQTRLKQS
HHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYFALFNLPKKYIIDKFLLSKNFYKLQLKFHPDLFIHHSESKKRIVLQKSIEINKGYKI
CCCHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCHHH
LQDSLNRAIHLLFLNGFKISKEKVLSEDNSFLKKYFFLYEELDFLLKNNCDEVQIDIFFK
HHHHHHHHEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
KIRNKIDNYEKIIEIKFNEKKWDDIIKLITKLLFFKKIQTRLKQS
HHHHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438