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The map label for this gene is dut

Identifier: 21672802

GI number: 21672802

Start: 593854

End: 594333

Strand: Reverse

Name: dut

Synonym: BUsg540

Alternate gene names: 21672802

Gene position: 594333-593854 (Counterclockwise)

Preceding gene: 21672803

Following gene: 161485723

Centisome position: 92.65

GC content: 27.5

Gene sequence:

>480_bases
ATGCAAAAACATTTGATTAATAATAATATTAAAATCAGAATTCTAGATTCTAATATAAAAAAAAATAGTAGTTTTTTTTT
ACCCCAATATGCAACTCCTGGATCTTCCGGTTTAGATCTTAGAGCAAGTATAAAGAAAAAAATATCTTTACCTTCTAAAG
AAGTTATTTTAGTTCCAACTGGAATAGCAATACATATTGATAATCCTTATATTACAGCTTTAGTTTTACCTCGTTCTGGA
TTAGGTCATAAAAATGGGATTATCTTAGGTAATTTGATTGGTTTAATTGATTCAGATTATCAAGGTGAATTAATGATATC
GCTTTGGAATCGTAGTAAAAATATTTTTTTTATTAATCCCTATGATAGAATTGCTCAGATGATATTTGTTCCAATTATCA
GACCTACATTTTCTATTGTTGATGATTTTGAAGAAACCGTACGTTTTGGAAAAGGATTTGGTCATTCAGGTGTTCAATAA

Upstream 100 bases:

>100_bases
TAAATTATTCTTTATCTTCTTATCATAATATTTCATCTTTAATAAATCCAGGTTTTTTAGCCTCTAATTAGAAAATTTAT
AATTCATTAATAAAATATTT

Downstream 100 bases:

>100_bases
TAAATAAAAATGTAATATTTTACCTATTACTTCTTATTATTGCTAATTTTTTACGATATTCGATTAATTTATATGCATAT
TTTTTTAATTTTTTTTCTTC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQKHLINNNIKIRILDSNIKKNSSFFLPQYATPGSSGLDLRASIKKKISLPSKEVILVPTGIAIHIDNPYITALVLPRSG
LGHKNGIILGNLIGLIDSDYQGELMISLWNRSKNIFFINPYDRIAQMIFVPIIRPTFSIVDDFEETVRFGKGFGHSGVQ

Sequences:

>Translated_159_residues
MQKHLINNNIKIRILDSNIKKNSSFFLPQYATPGSSGLDLRASIKKKISLPSKEVILVPTGIAIHIDNPYITALVLPRSG
LGHKNGIILGNLIGLIDSDYQGELMISLWNRSKNIFFINPYDRIAQMIFVPIIRPTFSIVDDFEETVRFGKGFGHSGVQ
>Mature_159_residues
MQKHLINNNIKIRILDSNIKKNSSFFLPQYATPGSSGLDLRASIKKKISLPSKEVILVPTGIAIHIDNPYITALVLPRSG
LGHKNGIILGNLIGLIDSDYQGELMISLWNRSKNIFFINPYDRIAQMIFVPIIRPTFSIVDDFEETVRFGKGFGHSGVQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=58, Blast_Score=185, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71988561, Length=138, Percent_Identity=36.231884057971, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319729, Length=139, Percent_Identity=35.2517985611511, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=35.3383458646617, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=35.3383458646617, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_BUCAP (Q8K921)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660869.1
- ProteinModelPortal:   Q8K921
- SMR:   Q8K921
- EnsemblBacteria:   EBBUCT00000000372
- GeneID:   1005571
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg540
- GeneTree:   EBGT00050000007746
- HOGENOM:   HBG436079
- OMA:   NEAYTIN
- ProtClustDB:   PRK00601
- BioCyc:   BAPH198804:BUSG540-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 17763; Mature: 17763

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: BINDING 91-91 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKHLINNNIKIRILDSNIKKNSSFFLPQYATPGSSGLDLRASIKKKISLPSKEVILVPT
CCCCEECCCCEEEEEECCCCCCCCEECCCCCCCCCCCCEEHEEHHHHCCCCCCCEEEEEC
GIAIHIDNPYITALVLPRSGLGHKNGIILGNLIGLIDSDYQGELMISLWNRSKNIFFINP
CEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEECC
YDRIAQMIFVPIIRPTFSIVDDFEETVRFGKGFGHSGVQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKHLINNNIKIRILDSNIKKNSSFFLPQYATPGSSGLDLRASIKKKISLPSKEVILVPT
CCCCEECCCCEEEEEECCCCCCCCEECCCCCCCCCCCCEEHEEHHHHCCCCCCCEEEEEC
GIAIHIDNPYITALVLPRSGLGHKNGIILGNLIGLIDSDYQGELMISLWNRSKNIFFINP
CEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEECC
YDRIAQMIFVPIIRPTFSIVDDFEETVRFGKGFGHSGVQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438