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The map label for this gene is sbcB

Identifier: 161485723

GI number: 161485723

Start: 590975

End: 592423

Strand: Reverse

Name: sbcB

Synonym: BUsg537

Alternate gene names: 161485723

Gene position: 592423-590975 (Counterclockwise)

Preceding gene: 21672802

Following gene: 21672796

Centisome position: 92.36

GC content: 20.84

Gene sequence:

>1449_bases
ATGAAAAATATTTTAAAAAAAAATGAAGTAAACTTTTTATTTTATGATTATGAAACTTTTGGAATACATACATCTCTAGA
TAAACCAGCTCAATTTTCTTCAATTAGAACAGATAAAAATTTTAATATTATGGAAGAACCAAAGTGTTTTTATTGTTTTC
CATCAGATGATTACTTACCTGATCCTTCTTCAATTTTAATTACAGGAATTACTCCTGAATATACAGAAAAACATGGTTTT
AATGAATACAAATTTTCTAAAAAAATACATGATGTTCTGTTAAAACCAAATACTTGTATTATAGGTTATAATAATATTAA
TTTTGATGATGAAATTACAAGAAATATATTTTATCGCAATTTTTTAGATCCTTATGAATGGAGTTGGAAAAATAATAATT
CTCGTTGGGATTTATTAAACGTTGTAAGAGCATGTTATGCTTTAAGACCAAGTGGTATACAATGGCCTAAAAATGAATTT
GGACTACCTGTTTTTAAACTTTCAGATTTAACTCAAAAAAATAATATATCACATTATAATGCTCATGATGCTACATCAGA
TGTATATGCTACTATTGAATTAGCCAAACTAATAAAAAGAAAACAACCAAAATTATTTGATTTTTTTTTCAAATACAGAA
AAAAAAATGAATTATGTAGGTTAATTGATATAGAAAAATTTACCCCAATTATATATGTTTCTAGTTATTTTGGAGCTTTA
CGTCAGAATATGAGTTGTATATTACCTTTGTCATGGGACCTACATAATAAAAACATATTAATTTCAATTGATTTATTTAA
AGATATTGACAAACTAATTGTTTTTTGCAAGACAGTATCTATTAGTAATATTTCAATTAAAGATTTATTTGATCTAGGAA
TAGTATTAGTATATTTGAATCGCTGTCCTATTTTGGCACCAATAAAAGTGATTAGAAAAGAAGATACTAATCGATTAAAC
TTTAAAAAGTATTTTTACTATAAGAAAATAAATCTAGTGAAAAAAAATTATTTTTTAATAGATTTAGTTAAAAATGTTTT
ATTAAAAAAAAATGAAAATAAAAATTCTTTAAATGTAGATCTACAAATATATGATTCTTTTTTTAATTTTCATGATAAAA
ATTTAATAAAAAAAATAAGTCTAACTAAATCGAGTGATTTAAAAAAGATGAAGTTAGATTTTAAAGATCCTCGTTTAAAA
GAGTTATTTTTTCGTTATAAAGCACGTAATTTTTTTAATATACTAAAAAATGATGAAAAAAAGGAATGGATTAATTATTG
TTTAAAAACTTTAAATTCTTTTTTTTTAACAGGATATATAGATAAAATTGAAAGTCTTTTAAAAATTTATTCTTATGATG
TAAAAAAAAATAATTTATTATCTGATTTATTAAAGTATGTTTTTAAAAAATATAAAAAAGTGTTTTATAAAAATATTAAT
TTAAGTTAA

Upstream 100 bases:

>100_bases
CATTATATTTAAAAAACCTTTTTCAGTTATAGTGAATATTTTTAATAATATTTTAGAGTTTAACAGTTTTTATTCATTAA
AACTAAAAATGAAAAACAAT

Downstream 100 bases:

>100_bases
TTTCTTGAATTTTTTTAAATTTATATTTTTTTAAAAAAAATACTAGTTGTTTTATTTGTTTATTATATTTCGAACATAAT
AAAATATTTTTTTTTATTTT

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKNILKKNEVNFLFYDYETFGIHTSLDKPAQFSSIRTDKNFNIMEEPKCFYCFPSDDYLPDPSSILITGITPEYTEKHGF
NEYKFSKKIHDVLLKPNTCIIGYNNINFDDEITRNIFYRNFLDPYEWSWKNNNSRWDLLNVVRACYALRPSGIQWPKNEF
GLPVFKLSDLTQKNNISHYNAHDATSDVYATIELAKLIKRKQPKLFDFFFKYRKKNELCRLIDIEKFTPIIYVSSYFGAL
RQNMSCILPLSWDLHNKNILISIDLFKDIDKLIVFCKTVSISNISIKDLFDLGIVLVYLNRCPILAPIKVIRKEDTNRLN
FKKYFYYKKINLVKKNYFLIDLVKNVLLKKNENKNSLNVDLQIYDSFFNFHDKNLIKKISLTKSSDLKKMKLDFKDPRLK
ELFFRYKARNFFNILKNDEKKEWINYCLKTLNSFFLTGYIDKIESLLKIYSYDVKKNNLLSDLLKYVFKKYKKVFYKNIN
LS

Sequences:

>Translated_482_residues
MKNILKKNEVNFLFYDYETFGIHTSLDKPAQFSSIRTDKNFNIMEEPKCFYCFPSDDYLPDPSSILITGITPEYTEKHGF
NEYKFSKKIHDVLLKPNTCIIGYNNINFDDEITRNIFYRNFLDPYEWSWKNNNSRWDLLNVVRACYALRPSGIQWPKNEF
GLPVFKLSDLTQKNNISHYNAHDATSDVYATIELAKLIKRKQPKLFDFFFKYRKKNELCRLIDIEKFTPIIYVSSYFGAL
RQNMSCILPLSWDLHNKNILISIDLFKDIDKLIVFCKTVSISNISIKDLFDLGIVLVYLNRCPILAPIKVIRKEDTNRLN
FKKYFYYKKINLVKKNYFLIDLVKNVLLKKNENKNSLNVDLQIYDSFFNFHDKNLIKKISLTKSSDLKKMKLDFKDPRLK
ELFFRYKARNFFNILKNDEKKEWINYCLKTLNSFFLTGYIDKIESLLKIYSYDVKKNNLLSDLLKYVFKKYKKVFYKNIN
LS
>Mature_482_residues
MKNILKKNEVNFLFYDYETFGIHTSLDKPAQFSSIRTDKNFNIMEEPKCFYCFPSDDYLPDPSSILITGITPEYTEKHGF
NEYKFSKKIHDVLLKPNTCIIGYNNINFDDEITRNIFYRNFLDPYEWSWKNNNSRWDLLNVVRACYALRPSGIQWPKNEF
GLPVFKLSDLTQKNNISHYNAHDATSDVYATIELAKLIKRKQPKLFDFFFKYRKKNELCRLIDIEKFTPIIYVSSYFGAL
RQNMSCILPLSWDLHNKNILISIDLFKDIDKLIVFCKTVSISNISIKDLFDLGIVLVYLNRCPILAPIKVIRKEDTNRLN
FKKYFYYKKINLVKKNYFLIDLVKNVLLKKNENKNSLNVDLQIYDSFFNFHDKNLIKKISLTKSSDLKKMKLDFKDPRLK
ELFFRYKARNFFNILKNDEKKEWINYCLKTLNSFFLTGYIDKIESLLKIYSYDVKKNNLLSDLLKYVFKKYKKVFYKNIN
LS

Specific function: Also functions as a DNA deoxyribophosphodiesterase that release deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=466, Percent_Identity=44.206008583691, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EX1_BUCAP (Q8K923)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660867.3
- ProteinModelPortal:   Q8K923
- SMR:   Q8K923
- EnsemblBacteria:   EBBUCT00000000139
- GeneID:   1005554
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg537
- GeneTree:   EBGT00050000007730
- HOGENOM:   HBG366231
- OMA:   PYAREWQ
- ProtClustDB:   PRK11779
- BioCyc:   BAPH198804:BUSG537-MONOMER
- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C; SSF53098 RNaseH_fold

EC number: =3.1.11.1

Molecular weight: Translated: 57566; Mature: 57566

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILKKNEVNFLFYDYETFGIHTSLDKPAQFSSIRTDKNFNIMEEPKCFYCFPSDDYLP
CCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
DPSSILITGITPEYTEKHGFNEYKFSKKIHDVLLKPNTCIIGYNNINFDDEITRNIFYRN
CCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
FLDPYEWSWKNNNSRWDLLNVVRACYALRPSGIQWPKNEFGLPVFKLSDLTQKNNISHYN
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEECCCHHHCCCCCCC
AHDATSDVYATIELAKLIKRKQPKLFDFFFKYRKKNELCRLIDIEKFTPIIYVSSYFGAL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHCCCEEEHHHHHHHH
RQNMSCILPLSWDLHNKNILISIDLFKDIDKLIVFCKTVSISNISIKDLFDLGIVLVYLN
HCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHH
RCPILAPIKVIRKEDTNRLNFKKYFYYKKINLVKKNYFLIDLVKNVLLKKNENKNSLNVD
CCCCHHHHHHHHCCCCCCCCHHHEEEEEEEEEHCCCHHHHHHHHHHHHCCCCCCCCEEEE
LQIYDSFFNFHDKNLIKKISLTKSSDLKKMKLDFKDPRLKELFFRYKARNFFNILKNDEK
EEEEEHHHCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
KEWINYCLKTLNSFFLTGYIDKIESLLKIYSYDVKKNNLLSDLLKYVFKKYKKVFYKNIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LS
CC
>Mature Secondary Structure
MKNILKKNEVNFLFYDYETFGIHTSLDKPAQFSSIRTDKNFNIMEEPKCFYCFPSDDYLP
CCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
DPSSILITGITPEYTEKHGFNEYKFSKKIHDVLLKPNTCIIGYNNINFDDEITRNIFYRN
CCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
FLDPYEWSWKNNNSRWDLLNVVRACYALRPSGIQWPKNEFGLPVFKLSDLTQKNNISHYN
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEECCCHHHCCCCCCC
AHDATSDVYATIELAKLIKRKQPKLFDFFFKYRKKNELCRLIDIEKFTPIIYVSSYFGAL
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHCCCEEEHHHHHHHH
RQNMSCILPLSWDLHNKNILISIDLFKDIDKLIVFCKTVSISNISIKDLFDLGIVLVYLN
HCCCEEEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHH
RCPILAPIKVIRKEDTNRLNFKKYFYYKKINLVKKNYFLIDLVKNVLLKKNENKNSLNVD
CCCCHHHHHHHHCCCCCCCCHHHEEEEEEEEEHCCCHHHHHHHHHHHHCCCCCCCCEEEE
LQIYDSFFNFHDKNLIKKISLTKSSDLKKMKLDFKDPRLKELFFRYKARNFFNILKNDEK
EEEEEHHHCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
KEWINYCLKTLNSFFLTGYIDKIESLLKIYSYDVKKNNLLSDLLKYVFKKYKKVFYKNIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438