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The map label for this gene is aroK

Identifier: 21672785

GI number: 21672785

Start: 574220

End: 574741

Strand: Reverse

Name: aroK

Synonym: BUsg520

Alternate gene names: 21672785

Gene position: 574741-574220 (Counterclockwise)

Preceding gene: 21672786

Following gene: 21672784

Centisome position: 89.6

GC content: 28.93

Gene sequence:

>522_bases
ATGGCAGAAAAACGAAACATTTTTTTAATTGGACCTATGGGAGCTGGTAAAAGTACTATTGGTCGTCAGTTGTCTCAACA
GCTAAATATGGAATTTTTTGATTCTGATCAAGAAATTGAAAAACGTACTGGAGCTAATATAAGTTGGGTTTTTGATGTTG
AAGGTGAACATGGTTTTCGTCAAAGAGAAGTGAAAGTTATTGATGAACTTACAAAAAAACAAGGTATTGTTCTTGCTACA
GGAGGAGGTTCAGTAAAATTTAAAGAAAATCGTAATATTTTATCTGCTCGCGGAATTGTTATATATTTAGAAACTACTAT
TGAAAAACAGTTATCACGTACAAAAAGAGATAAAAAAAGACCATTATTGCAGTCTAATATTTCAAATCGTACTGTATTAG
AAAATTTAGCATATGAAAGAAATCCATTATATGAAGAAATAGCAGATTTTAAAATTCAAACTGATAATCAAAGTGCTAAA
TCTGTTGCATATAGTATTATTCATTTATTAGAAAAAATGTAA

Upstream 100 bases:

>100_bases
TGTACGTAAAAATTTATTCTAATTGTAGCTTTTTTTATGTCTTGCTAGCAAAATTTGCTAAGCATTACTTTTATTAACGA
ATTATCTTAGTAATAAAAAT

Downstream 100 bases:

>100_bases
TGGAAATTTAAAAAAAATGTTTATAGAGTATAAAAATTATGGAACAATTAAAAGTTGTTTTAGGAAAACGTAGCTATCCT
ATTAATATTGGATCTAGTAT

Product: shikimate kinase I

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAEKRNIFLIGPMGAGKSTIGRQLSQQLNMEFFDSDQEIEKRTGANISWVFDVEGEHGFRQREVKVIDELTKKQGIVLAT
GGGSVKFKENRNILSARGIVIYLETTIEKQLSRTKRDKKRPLLQSNISNRTVLENLAYERNPLYEEIADFKIQTDNQSAK
SVAYSIIHLLEKM

Sequences:

>Translated_173_residues
MAEKRNIFLIGPMGAGKSTIGRQLSQQLNMEFFDSDQEIEKRTGANISWVFDVEGEHGFRQREVKVIDELTKKQGIVLAT
GGGSVKFKENRNILSARGIVIYLETTIEKQLSRTKRDKKRPLLQSNISNRTVLENLAYERNPLYEEIADFKIQTDNQSAK
SVAYSIIHLLEKM
>Mature_172_residues
AEKRNIFLIGPMGAGKSTIGRQLSQQLNMEFFDSDQEIEKRTGANISWVFDVEGEHGFRQREVKVIDELTKKQGIVLATG
GGSVKFKENRNILSARGIVIYLETTIEKQLSRTKRDKKRPLLQSNISNRTVLENLAYERNPLYEEIADFKIQTDNQSAKS
VAYSIIHLLEKM

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=171, Percent_Identity=78.3625730994152, Blast_Score=277, Evalue=2e-76,
Organism=Escherichia coli, GI1786587, Length=160, Percent_Identity=32.5, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_BUCAP (Q8K938)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660852.1
- ProteinModelPortal:   Q8K938
- SMR:   Q8K938
- EnsemblBacteria:   EBBUCT00000000249
- GeneID:   1005546
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg520
- GeneTree:   EBGT00050000007771
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK05057
- BioCyc:   BAPH198804:BUSG520-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19748; Mature: 19617

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 140-140 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRNIFLIGPMGAGKSTIGRQLSQQLNMEFFDSDQEIEKRTGANISWVFDVEGEHGFR
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHCCCCEEEEEEECCCCCCH
QREVKVIDELTKKQGIVLATGGGSVKFKENRNILSARGIVIYLETTIEKQLSRTKRDKKR
HHHHHHHHHHHHHCCEEEEECCCEEEECCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHC
PLLQSNISNRTVLENLAYERNPLYEEIADFKIQTDNQSAKSVAYSIIHLLEKM
CHHHCCCCCHHHHHHHHHCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKRNIFLIGPMGAGKSTIGRQLSQQLNMEFFDSDQEIEKRTGANISWVFDVEGEHGFR
CCCCCEEEEECCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHCCCCEEEEEEECCCCCCH
QREVKVIDELTKKQGIVLATGGGSVKFKENRNILSARGIVIYLETTIEKQLSRTKRDKKR
HHHHHHHHHHHHHCCEEEEECCCEEEECCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHC
PLLQSNISNRTVLENLAYERNPLYEEIADFKIQTDNQSAKSVAYSIIHLLEKM
CHHHCCCCCHHHHHHHHHCCCCHHHHHHCCEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438