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The map label for this gene is cysS

Identifier: 21672736

GI number: 21672736

Start: 537706

End: 539097

Strand: Reverse

Name: cysS

Synonym: BUsg471

Alternate gene names: 21672736

Gene position: 539097-537706 (Counterclockwise)

Preceding gene: 21672738

Following gene: 21672728

Centisome position: 84.04

GC content: 24.35

Gene sequence:

>1392_bases
ATGTTAAAAATTTTTAATACCGCAACATCTAAAAAAGAAATTTTTAAACCTATTAAAAACAAAAAAATTAATTTATATGT
TTGTGGAGTTACTGTATATGATTTTTGTCATATTGGTCACGCACGTACTTTCGTTGTTTTTGACATGATAGTGCGTTATT
TACGATTAATAGGATTTCAAGTTAAATATATTCGTAATATTACAGATATTGATGATAAAATTATTTTAAAATCATTAAAA
GAAAAAATTGATATTAGTGCTTTTACAAATATTATGATAAAAGAAATGAATAAAGATTTTTCTTTATTAGGCATTTCCCC
TCCTGATGAAGAACCTCGCGTTACTAATTATATTATTGATATAATTAAAGTTATTGAAAAACTAATTAAAAATAAAAACG
CCTATGTTAATGATCATGGAGATGTAATTTTCTCAATAAATAGTGATAAATTTTACGGAACTTTATCTCGTCAATCTTTA
GATAAATTAATATCTGGTACACGTATTTCTATAGATAAAGAAAAAAAGAATTCATCAGATTTTATACTTTGGAAGAAATC
TAAAAAAGAAGAAAAATTTTCTTGGAATTCACCGTGGGGTAAAGGACGTCCCGGTTGGCATATTGAATGTACCGCTATCA
CTAATGTTTTTTTTAAAGATTATATAGATATTCATGGAGGTGGTTCAGATCTTCTTTTTCCTCATCATGAAAATGAAATA
TCCCAGTCAAGATGTTTAAATAAAAATTTCAGAGTAAAGTTTTGGATGCATTCAGGATTAGTAATAATTAAAAATCAAAA
AATGTCTAAATCTTTAGGTAATTCATTTTTTTTAAAAGATGTTCTAGAGAAATATGAAGCTGAAGTTTTACGTTTTTTCT
TTTTATCTACTCATTATCGACATCCTATTTATTATACGGAAGAAAATTTAAAGAAATCTGAAATGTCACTAGAATATTTA
TATGTAACATTGTATGGTACAAAACCTATTTTTGATCCTATAGAAGGTGTGAATTTTGAGATAGATTTTTTTGAAGCTTT
AAATGATGATTTTAACACACCTCAGGCATTTTCTATTTTATTTAAATTAGCCCGAAAAATTAACTATTTAAAAAAGATAG
ATTTATCTAAATCAAATTTTTTTGCTTTTAGATTACAGAAACTAGCTGGTTATTTAGGTTTTTTATTAAATACATCACAA
TATTTTTTGCAAAAAAAATTACCTTTTGATGAAGAAATTATAAAAAAAATTGAATCATTAATTGAAAAAAGAAATTTTGC
TAGAAAATCAAAATTATGGAAAGAAGCTGATAAATTACGTGATAAATTAATAAAATTAAATGTTATCTTAGAAGATTTGC
CTGAAAAAACCTTATGGCGTTTTAAACGATAA

Upstream 100 bases:

>100_bases
TTTATAAAAAAATAGATATTAATATATCTAATTAGTTTAACATATTGATATTATAGAAAATAATATATTTTATTTTTTTT
AAATATTTCATGAGAAATTT

Downstream 100 bases:

>100_bases
CATATTTATTTATTGCATTTAAAATTCATGATATTTTTCACAGGCCTCTAAAGTATTTTCTAACAACGTTATAACTGTGA
TTGGACCAACCCCCCCTGGT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLKIFNTATSKKEIFKPIKNKKINLYVCGVTVYDFCHIGHARTFVVFDMIVRYLRLIGFQVKYIRNITDIDDKIILKSLK
EKIDISAFTNIMIKEMNKDFSLLGISPPDEEPRVTNYIIDIIKVIEKLIKNKNAYVNDHGDVIFSINSDKFYGTLSRQSL
DKLISGTRISIDKEKKNSSDFILWKKSKKEEKFSWNSPWGKGRPGWHIECTAITNVFFKDYIDIHGGGSDLLFPHHENEI
SQSRCLNKNFRVKFWMHSGLVIIKNQKMSKSLGNSFFLKDVLEKYEAEVLRFFFLSTHYRHPIYYTEENLKKSEMSLEYL
YVTLYGTKPIFDPIEGVNFEIDFFEALNDDFNTPQAFSILFKLARKINYLKKIDLSKSNFFAFRLQKLAGYLGFLLNTSQ
YFLQKKLPFDEEIIKKIESLIEKRNFARKSKLWKEADKLRDKLIKLNVILEDLPEKTLWRFKR

Sequences:

>Translated_463_residues
MLKIFNTATSKKEIFKPIKNKKINLYVCGVTVYDFCHIGHARTFVVFDMIVRYLRLIGFQVKYIRNITDIDDKIILKSLK
EKIDISAFTNIMIKEMNKDFSLLGISPPDEEPRVTNYIIDIIKVIEKLIKNKNAYVNDHGDVIFSINSDKFYGTLSRQSL
DKLISGTRISIDKEKKNSSDFILWKKSKKEEKFSWNSPWGKGRPGWHIECTAITNVFFKDYIDIHGGGSDLLFPHHENEI
SQSRCLNKNFRVKFWMHSGLVIIKNQKMSKSLGNSFFLKDVLEKYEAEVLRFFFLSTHYRHPIYYTEENLKKSEMSLEYL
YVTLYGTKPIFDPIEGVNFEIDFFEALNDDFNTPQAFSILFKLARKINYLKKIDLSKSNFFAFRLQKLAGYLGFLLNTSQ
YFLQKKLPFDEEIIKKIESLIEKRNFARKSKLWKEADKLRDKLIKLNVILEDLPEKTLWRFKR
>Mature_463_residues
MLKIFNTATSKKEIFKPIKNKKINLYVCGVTVYDFCHIGHARTFVVFDMIVRYLRLIGFQVKYIRNITDIDDKIILKSLK
EKIDISAFTNIMIKEMNKDFSLLGISPPDEEPRVTNYIIDIIKVIEKLIKNKNAYVNDHGDVIFSINSDKFYGTLSRQSL
DKLISGTRISIDKEKKNSSDFILWKKSKKEEKFSWNSPWGKGRPGWHIECTAITNVFFKDYIDIHGGGSDLLFPHHENEI
SQSRCLNKNFRVKFWMHSGLVIIKNQKMSKSLGNSFFLKDVLEKYEAEVLRFFFLSTHYRHPIYYTEENLKKSEMSLEYL
YVTLYGTKPIFDPIEGVNFEIDFFEALNDDFNTPQAFSILFKLARKINYLKKIDLSKSNFFAFRLQKLAGYLGFLLNTSQ
YFLQKKLPFDEEIIKKIESLIEKRNFARKSKLWKEADKLRDKLIKLNVILEDLPEKTLWRFKR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=386, Percent_Identity=35.7512953367876, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI62240992, Length=242, Percent_Identity=38.4297520661157, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI303304987, Length=242, Percent_Identity=38.4297520661157, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI21269877, Length=242, Percent_Identity=38.4297520661157, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI10835051, Length=242, Percent_Identity=38.4297520661157, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1786737, Length=462, Percent_Identity=51.5151515151515, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17509795, Length=239, Percent_Identity=35.5648535564854, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17509797, Length=239, Percent_Identity=35.5648535564854, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324082, Length=306, Percent_Identity=33.9869281045752, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=31.8811881188119, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI19922364, Length=242, Percent_Identity=38.4297520661157, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BUCAP (P46241)

Other databases:

- EMBL:   AE013218
- EMBL:   U09230
- RefSeq:   NP_660803.1
- ProteinModelPortal:   P46241
- SMR:   P46241
- EnsemblBacteria:   EBBUCT00000000008
- GeneID:   1005696
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg471
- GeneTree:   EBGT00050000008180
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   BAPH198804:BUSG471-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54634; Mature: 54634

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIFNTATSKKEIFKPIKNKKINLYVCGVTVYDFCHIGHARTFVVFDMIVRYLRLIGFQ
CEEEECCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VKYIRNITDIDDKIILKSLKEKIDISAFTNIMIKEMNKDFSLLGISPPDEEPRVTNYIID
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
IIKVIEKLIKNKNAYVNDHGDVIFSINSDKFYGTLSRQSLDKLISGTRISIDKEKKNSSD
HHHHHHHHHCCCCCEEECCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCC
FILWKKSKKEEKFSWNSPWGKGRPGWHIECTAITNVFFKDYIDIHGGGSDLLFPHHENEI
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEECCCCCCCCCCCCCHH
SQSRCLNKNFRVKFWMHSGLVIIKNQKMSKSLGNSFFLKDVLEKYEAEVLRFFFLSTHYR
HHHHHCCCCCEEEEEEECCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HPIYYTEENLKKSEMSLEYLYVTLYGTKPIFDPIEGVNFEIDFFEALNDDFNTPQAFSIL
CCEEEECCCCCHHHCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHH
FKLARKINYLKKIDLSKSNFFAFRLQKLAGYLGFLLNTSQYFLQKKLPFDEEIIKKIESL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
IEKRNFARKSKLWKEADKLRDKLIKLNVILEDLPEKTLWRFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MLKIFNTATSKKEIFKPIKNKKINLYVCGVTVYDFCHIGHARTFVVFDMIVRYLRLIGFQ
CEEEECCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VKYIRNITDIDDKIILKSLKEKIDISAFTNIMIKEMNKDFSLLGISPPDEEPRVTNYIID
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
IIKVIEKLIKNKNAYVNDHGDVIFSINSDKFYGTLSRQSLDKLISGTRISIDKEKKNSSD
HHHHHHHHHCCCCCEEECCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCC
FILWKKSKKEEKFSWNSPWGKGRPGWHIECTAITNVFFKDYIDIHGGGSDLLFPHHENEI
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEECCCCCCCCCCCCCHH
SQSRCLNKNFRVKFWMHSGLVIIKNQKMSKSLGNSFFLKDVLEKYEAEVLRFFFLSTHYR
HHHHHCCCCCEEEEEEECCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HPIYYTEENLKKSEMSLEYLYVTLYGTKPIFDPIEGVNFEIDFFEALNDDFNTPQAFSIL
CCEEEECCCCCHHHCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHH
FKLARKINYLKKIDLSKSNFFAFRLQKLAGYLGFLLNTSQYFLQKKLPFDEEIIKKIESL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
IEKRNFARKSKLWKEADKLRDKLIKLNVILEDLPEKTLWRFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438; 7535281