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The map label for this gene is folD

Identifier: 21672735

GI number: 21672735

Start: 536830

End: 537687

Strand: Direct

Name: folD

Synonym: BUsg470

Alternate gene names: 21672735

Gene position: 536830-537687 (Clockwise)

Preceding gene: 21672734

Following gene: 21672737

Centisome position: 83.69

GC content: 29.14

Gene sequence:

>858_bases
ATGTCTGCAATAATTATAGATGGCATCAAAATTGCAAAAAAAATAGAATTAAATCTTTTAAAAAAGATAGAAGAAAGGGA
AAAAAATAAAAAAAGGATACCAGGATTGGCAGTAATTTTAATAGGAAAAAATCCTGCTTCTGAAATTTATGTAAAAAGAA
AAATATCAGTATGTAAAAAAGTTGGATTTATTTCTAAATATTGGAGTTTTCCTATAAATGTTGATGAAAAAGATATATTG
AATCTTATCGAAAAATTAAACAACAACATTAATATAGACGGTATTTTAGTACAGTTACCTATACCTAAGCAAATAAATTA
TTATAAAATATTTAGTAGTATCAGACCTGATAAAGATGTAGATGGTTTTCATCCTTACAATACAGGCTCTTTATGTCAAA
GAAATCCTACTTTAAGAGCTTGTACACCTAAGGGGATCATCACAATGTTAAACTATACAAAAATTAAAACTCATGGACTA
AATGCTGTGATGGTTGGAGCTTCTAACATAGTAGGTAGACCTATGAGTATGGAACTATTATTAGCTGGATGTACAACTAC
AGTGACACATAGATTTACTAAAAACTTAAGACATCATATTAAAAACGCTGATCTTTTAGTAGTTGCAATTGGAAAACCAA
ATTTTCTACATGGAGATTGGATAAAAGAAGGTGCTATAGTAATAGATGTAGGTATTAATAAATTGAAAGATGGATCTATA
GTAGGTGATGTAGATTTTAAATCAGCATCTCTAAAAGCTGCTTATATCACTCCAGTACCAGGGGGGGTTGGTCCAATCAC
AGTTATAACGTTGTTAGAAAATACTTTAGAGGCCTGTGAAAAATATCATGAATTTTAA

Upstream 100 bases:

>100_bases
CAACTAAATTAATATGAATATTCTATATATTAATATAAAAAAAATTATATCAAAAATTAAAATGATTTTTCACTAAAAAA
AATAACTGAGCTACGAAATA

Downstream 100 bases:

>100_bases
ATGCAATAAATAAATATGTTATCGTTTAAAACGCCATAAGGTTTTTTCAGGCAAATCTTCTAAGATAACATTTAATTTTA
TTAATTTATCACGTAATTTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSAIIIDGIKIAKKIELNLLKKIEEREKNKKRIPGLAVILIGKNPASEIYVKRKISVCKKVGFISKYWSFPINVDEKDIL
NLIEKLNNNINIDGILVQLPIPKQINYYKIFSSIRPDKDVDGFHPYNTGSLCQRNPTLRACTPKGIITMLNYTKIKTHGL
NAVMVGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHIKNADLLVVAIGKPNFLHGDWIKEGAIVIDVGINKLKDGSI
VGDVDFKSASLKAAYITPVPGGVGPITVITLLENTLEACEKYHEF

Sequences:

>Translated_285_residues
MSAIIIDGIKIAKKIELNLLKKIEEREKNKKRIPGLAVILIGKNPASEIYVKRKISVCKKVGFISKYWSFPINVDEKDIL
NLIEKLNNNINIDGILVQLPIPKQINYYKIFSSIRPDKDVDGFHPYNTGSLCQRNPTLRACTPKGIITMLNYTKIKTHGL
NAVMVGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHIKNADLLVVAIGKPNFLHGDWIKEGAIVIDVGINKLKDGSI
VGDVDFKSASLKAAYITPVPGGVGPITVITLLENTLEACEKYHEF
>Mature_284_residues
SAIIIDGIKIAKKIELNLLKKIEEREKNKKRIPGLAVILIGKNPASEIYVKRKISVCKKVGFISKYWSFPINVDEKDILN
LIEKLNNNINIDGILVQLPIPKQINYYKIFSSIRPDKDVDGFHPYNTGSLCQRNPTLRACTPKGIITMLNYTKIKTHGLN
AVMVGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHIKNADLLVVAIGKPNFLHGDWIKEGAIVIDVGINKLKDGSIV
GDVDFKSASLKAAYITPVPGGVGPITVITLLENTLEACEKYHEF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=41.4965986394558, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.1972789115646, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=36.1774744027304, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI36796743, Length=207, Percent_Identity=25.1207729468599, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=62.6760563380282, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=35.958904109589, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=39.1608391608392, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=35.8803986710963, Blast_Score=195, Evalue=9e-51,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=41.8367346938776, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=41.8367346938776, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=36.3013698630137, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=36.3013698630137, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=36.3013698630137, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=36.3013698630137, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BUCAP (Q8K979)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660802.1
- ProteinModelPortal:   Q8K979
- SMR:   Q8K979
- EnsemblBacteria:   EBBUCT00000000315
- GeneID:   1005697
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg470
- GeneTree:   EBGT00050000007792
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK10792
- BioCyc:   BAPH198804:BUSG470-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31610; Mature: 31479

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIIIDGIKIAKKIELNLLKKIEEREKNKKRIPGLAVILIGKNPASEIYVKRKISVCKK
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VGFISKYWSFPINVDEKDILNLIEKLNNNINIDGILVQLPIPKQINYYKIFSSIRPDKDV
HCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNTGSLCQRNPTLRACTPKGIITMLNYTKIKTHGLNAVMVGASNIVGRPMSMELL
CCCCCCCCCCHHCCCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECHHHCCCCCEEEEE
LAGCTTTVTHRFTKNLRHHIKNADLLVVAIGKPNFLHGDWIKEGAIVIDVGINKLKDGSI
EECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCCEEEEECCCCCCCCCE
VGDVDFKSASLKAAYITPVPGGVGPITVITLLENTLEACEKYHEF
EECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAIIIDGIKIAKKIELNLLKKIEEREKNKKRIPGLAVILIGKNPASEIYVKRKISVCKK
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VGFISKYWSFPINVDEKDILNLIEKLNNNINIDGILVQLPIPKQINYYKIFSSIRPDKDV
HCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNTGSLCQRNPTLRACTPKGIITMLNYTKIKTHGLNAVMVGASNIVGRPMSMELL
CCCCCCCCCCHHCCCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECHHHCCCCCEEEEE
LAGCTTTVTHRFTKNLRHHIKNADLLVVAIGKPNFLHGDWIKEGAIVIDVGINKLKDGSI
EECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCCEEEEECCCCCCCCCE
VGDVDFKSASLKAAYITPVPGGVGPITVITLLENTLEACEKYHEF
EECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438