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The map label for this gene is thiL

Identifier: 21672709

GI number: 21672709

Start: 507207

End: 508178

Strand: Direct

Name: thiL

Synonym: BUsg444

Alternate gene names: 21672709

Gene position: 507207-508178 (Clockwise)

Preceding gene: 21672708

Following gene: 21672710

Centisome position: 79.07

GC content: 25.41

Gene sequence:

>972_bases
ATGGAATATAACGAATTTGAAATTATATCTAAATTTTTTAAAAATCATCAAAAAAAAGATAAAAATCAAATTAAGGGAAT
TGGAGACGATAGTGCCTTAATAAAAATACCAAAAAACAATCTTCTTGCAATTAGCACTGATACCTTGGTTGAGGGAAAGC
ACTTTTTAAAAAATATAACTCCTAAAGATTTAGCATATAAATCTGTTGCAGTTAATCTTAGTGATTTAGCTGCTATGGGA
GCAAAACCAACATGGATCACATTATCTATCACAATGCCAAAATCTGATAGTGTATGGCTAAAATCATTTAGTAAAAGTTT
TTTTAAAATTTTAAATAAATATAAATTAAATTTAATTGGAGGAGATACGAATTCGGGTCCCTTAAGTATCACATTAAGCA
TTTACGGATTAATAGAAGGAAAAAATGCACTACTACGAGGAAATGCTAAAAATGGAGATTTAATATATATCACTGGAAAT
CTTGGAGAAAGCGCTGCAGGTTTATCCTTACTTCAAAAAAAAACTTTTTTAAAAAACGTGAAAATATGCAATTATTTAAT
TAAAAAACATCTGAAACCTATTCCTAGAATTTCTGAAGGCATAGCATTAAGAAACATTGCTAATGCAGCTATCGATATAT
CAGACGGTTTAATTTCTGATTTAGGTCATATATTAAAAAATAGTAAATGTGGAGCTGATATTAATTTAAATACAATACCT
ATTTCTAAAATATTAACTGATAATTTTAAGATTGATGAATATTTAAACTGGGCTTTAAATTCTGGAGAAGATTATGAATT
GTGTTTTACCATCTCAAAAAAAAATATTAAAAAATTAAATGTAGCTATTAAAAAAAAAATAATTAAATGCACATGTATAG
GTTATATTACATCTGCAGAAAAGGGATTGAATTTGATACAAAATCAAAAAAAAATAATTTTTAAAAAATCAGGTTTTAAT
CATTTTATTTAA

Upstream 100 bases:

>100_bases
GTCTGGTACAAAAATGGGTAATAAGGGATCTGAAGCTGCATTAGCTGCATTAGAAATGATTAATATTATGAAAAAATTAA
AAAAAATAAATAATTAAATT

Downstream 100 bases:

>100_bases
TAAATATGAAAAATTGGTGAAATATGAAAGATAGATTTTATATGACAAGAGCAATAAAACTTAGTAAATTAGGAGAATTT
ACAACTTCTCCAAATCCTAA

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MEYNEFEIISKFFKNHQKKDKNQIKGIGDDSALIKIPKNNLLAISTDTLVEGKHFLKNITPKDLAYKSVAVNLSDLAAMG
AKPTWITLSITMPKSDSVWLKSFSKSFFKILNKYKLNLIGGDTNSGPLSITLSIYGLIEGKNALLRGNAKNGDLIYITGN
LGESAAGLSLLQKKTFLKNVKICNYLIKKHLKPIPRISEGIALRNIANAAIDISDGLISDLGHILKNSKCGADINLNTIP
ISKILTDNFKIDEYLNWALNSGEDYELCFTISKKNIKKLNVAIKKKIIKCTCIGYITSAEKGLNLIQNQKKIIFKKSGFN
HFI

Sequences:

>Translated_323_residues
MEYNEFEIISKFFKNHQKKDKNQIKGIGDDSALIKIPKNNLLAISTDTLVEGKHFLKNITPKDLAYKSVAVNLSDLAAMG
AKPTWITLSITMPKSDSVWLKSFSKSFFKILNKYKLNLIGGDTNSGPLSITLSIYGLIEGKNALLRGNAKNGDLIYITGN
LGESAAGLSLLQKKTFLKNVKICNYLIKKHLKPIPRISEGIALRNIANAAIDISDGLISDLGHILKNSKCGADINLNTIP
ISKILTDNFKIDEYLNWALNSGEDYELCFTISKKNIKKLNVAIKKKIIKCTCIGYITSAEKGLNLIQNQKKIIFKKSGFN
HFI
>Mature_323_residues
MEYNEFEIISKFFKNHQKKDKNQIKGIGDDSALIKIPKNNLLAISTDTLVEGKHFLKNITPKDLAYKSVAVNLSDLAAMG
AKPTWITLSITMPKSDSVWLKSFSKSFFKILNKYKLNLIGGDTNSGPLSITLSIYGLIEGKNALLRGNAKNGDLIYITGN
LGESAAGLSLLQKKTFLKNVKICNYLIKKHLKPIPRISEGIALRNIANAAIDISDGLISDLGHILKNSKCGADINLNTIP
ISKILTDNFKIDEYLNWALNSGEDYELCFTISKKNIKKLNVAIKKKIIKCTCIGYITSAEKGLNLIQNQKKIIFKKSGFN
HFI

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family

Homologues:

Organism=Escherichia coli, GI1786619, Length=322, Percent_Identity=51.2422360248447, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_BUCAP (Q8K9A5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660776.1
- ProteinModelPortal:   Q8K9A5
- SMR:   Q8K9A5
- EnsemblBacteria:   EBBUCT00000000530
- GeneID:   1005720
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg444
- GeneTree:   EBGT00050000008007
- HOGENOM:   HBG577902
- OMA:   MGAEPAW
- ProtClustDB:   CLSK316267
- BioCyc:   BAPH198804:BUSG444-MONOMER
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283
- PIRSF:   PIRSF005303
- TIGRFAMs:   TIGR01379

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =2.7.4.16

Molecular weight: Translated: 35838; Mature: 35838

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYNEFEIISKFFKNHQKKDKNQIKGIGDDSALIKIPKNNLLAISTDTLVEGKHFLKNIT
CCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCEEEEECCHHHCCHHHHHCCC
PKDLAYKSVAVNLSDLAAMGAKPTWITLSITMPKSDSVWLKSFSKSFFKILNKYKLNLIG
HHHHHHHHEEEEHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEE
GDTNSGPLSITLSIYGLIEGKNALLRGNAKNGDLIYITGNLGESAAGLSLLQKKTFLKNV
CCCCCCCEEEEEEEEEEEECCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KICNYLIKKHLKPIPRISEGIALRNIANAAIDISDGLISDLGHILKNSKCGADINLNTIP
HHHHHHHHHHCCCCCCHHCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCEEE
ISKILTDNFKIDEYLNWALNSGEDYELCFTISKKNIKKLNVAIKKKIIKCTCIGYITSAE
HHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEECCHH
KGLNLIQNQKKIIFKKSGFNHFI
HHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MEYNEFEIISKFFKNHQKKDKNQIKGIGDDSALIKIPKNNLLAISTDTLVEGKHFLKNIT
CCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCEEEEECCHHHCCHHHHHCCC
PKDLAYKSVAVNLSDLAAMGAKPTWITLSITMPKSDSVWLKSFSKSFFKILNKYKLNLIG
HHHHHHHHEEEEHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEE
GDTNSGPLSITLSIYGLIEGKNALLRGNAKNGDLIYITGNLGESAAGLSLLQKKTFLKNV
CCCCCCCEEEEEEEEEEEECCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KICNYLIKKHLKPIPRISEGIALRNIANAAIDISDGLISDLGHILKNSKCGADINLNTIP
HHHHHHHHHHCCCCCCHHCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCEEE
ISKILTDNFKIDEYLNWALNSGEDYELCFTISKKNIKKLNVAIKKKIIKCTCIGYITSAE
HHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEEECCHH
KGLNLIQNQKKIIFKKSGFNHFI
HHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438