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The map label for this gene is fba

Identifier: 21672701

GI number: 21672701

Start: 493144

End: 494223

Strand: Direct

Name: fba

Synonym: BUsg436

Alternate gene names: 21672701

Gene position: 493144-494223 (Clockwise)

Preceding gene: 21672700

Following gene: 21672702

Centisome position: 76.88

GC content: 27.69

Gene sequence:

>1080_bases
ATGAAAATATTAAATCTTATTCAACCTGGAGTTGTAACAGGTAATGAATGTCAGATAATATTCGAATTAGCTAAAAAAAA
GAAATTTGCAATACCGGCTGTTAATTGTATAGGTACGGATTCAATAAATGCAGTCTTAGAAACTGCTTCTAGGGTAAAGT
CTCCAGTTATTATACAATTTTCTTATGGAGGCGCTTCTTTTATTGCAGGTTATAAGAGAACATCTTCAAAAAATCCAGAA
GATCAGGCTATTCAAGGATCTATATCAGGTGCTCAACACGTACATTTAATGTCACAATACTATCAAATACCTGTAATACT
TCATACAGACCACTGTTCTAAGGAAATGTTACCATGGATTGATGGATTACTAGAAGAAGGTAAAAAACACTATAAAAATT
TTGGAAAACCTCTTTTCACTTCTCATATGATTGATTTATCAAAAGAACATTTAAAAGAAAATATTGCCATTTGCAGCAAT
TATTTAAAAAAAATTAAAAAAATAAATATGATTTTAGAAATTGAATTAGGATGCACAGGAGGAGAAGAAGACGGTATAGA
TAACAGTAACATAGATAAAAAACTACTTTATACACAACCTAAAGATGTTAATTATGCATATAAAAAATTAAGTAAAATTA
GCACAAATTTTACCATTGCAGCTTCATTTGGAAATGTACATGGCGTATATCAACCTGGAAATGTTAATCTTAAACCAAAT
ATTCTAAAAGAATCTCAGGAATATGTAAGTACTAAACATAATTTAAAAAAGCTTCCATTAAATTTAGTATTTCATGGTGG
TTCAGGTTCAGATTTAAAAGAAATACATGAATCTATTGAATACGGTATAGTAAAAATGAATATTGACACCGATATACAGT
GGGCAAGTTGGAAAGGAGTTTTCAATTTTTATAAAAAAAATAAAGACTTCTTACAAAAACAAATAGGAAATAAAAATGGT
AAAAACATACCTAATAAAAAATATTATGATCCTAGATCATGGATAAGAGAATCTCAAGAATCAATGTCTAAAAGATTAGA
AAAAACATTTAAGGATCTTAATGCTTTTAATATTTTATAA

Upstream 100 bases:

>100_bases
GGGAAAAAATTACCTGCCATGAAAATGTTAGAAGAAAATTTTCACAAATAAGTTTTAAACAATTCATTGTAATAAAAAAT
TCAATATAGCAGGAAAAAAA

Downstream 100 bases:

>100_bases
AAAAAAATAAAGAAATTTTATATTTAAGTCCGGGGAATAAATAAAAATATTCTCCGAAATTTTTTAAAACATCTATCTTA
TATAATCTATAAAAATGTTT

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; Fructose-bisphosphate aldolase class II

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKILNLIQPGVVTGNECQIIFELAKKKKFAIPAVNCIGTDSINAVLETASRVKSPVIIQFSYGGASFIAGYKRTSSKNPE
DQAIQGSISGAQHVHLMSQYYQIPVILHTDHCSKEMLPWIDGLLEEGKKHYKNFGKPLFTSHMIDLSKEHLKENIAICSN
YLKKIKKINMILEIELGCTGGEEDGIDNSNIDKKLLYTQPKDVNYAYKKLSKISTNFTIAASFGNVHGVYQPGNVNLKPN
ILKESQEYVSTKHNLKKLPLNLVFHGGSGSDLKEIHESIEYGIVKMNIDTDIQWASWKGVFNFYKKNKDFLQKQIGNKNG
KNIPNKKYYDPRSWIRESQESMSKRLEKTFKDLNAFNIL

Sequences:

>Translated_359_residues
MKILNLIQPGVVTGNECQIIFELAKKKKFAIPAVNCIGTDSINAVLETASRVKSPVIIQFSYGGASFIAGYKRTSSKNPE
DQAIQGSISGAQHVHLMSQYYQIPVILHTDHCSKEMLPWIDGLLEEGKKHYKNFGKPLFTSHMIDLSKEHLKENIAICSN
YLKKIKKINMILEIELGCTGGEEDGIDNSNIDKKLLYTQPKDVNYAYKKLSKISTNFTIAASFGNVHGVYQPGNVNLKPN
ILKESQEYVSTKHNLKKLPLNLVFHGGSGSDLKEIHESIEYGIVKMNIDTDIQWASWKGVFNFYKKNKDFLQKQIGNKNG
KNIPNKKYYDPRSWIRESQESMSKRLEKTFKDLNAFNIL
>Mature_359_residues
MKILNLIQPGVVTGNECQIIFELAKKKKFAIPAVNCIGTDSINAVLETASRVKSPVIIQFSYGGASFIAGYKRTSSKNPE
DQAIQGSISGAQHVHLMSQYYQIPVILHTDHCSKEMLPWIDGLLEEGKKHYKNFGKPLFTSHMIDLSKEHLKENIAICSN
YLKKIKKINMILEIELGCTGGEEDGIDNSNIDKKLLYTQPKDVNYAYKKLSKISTNFTIAASFGNVHGVYQPGNVNLKPN
ILKESQEYVSTKHNLKKLPLNLVFHGGSGSDLKEIHESIEYGIVKMNIDTDIQWASWKGVFNFYKKNKDFLQKQIGNKNG
KNIPNKKYYDPRSWIRESQESMSKRLEKTFKDLNAFNIL

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789293, Length=358, Percent_Identity=65.3631284916201, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87082041, Length=329, Percent_Identity=23.1003039513678, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788072, Length=292, Percent_Identity=26.3698630136986, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322790, Length=351, Percent_Identity=44.4444444444444, Blast_Score=334, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_BUCAP (Q8K9B2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660768.1
- ProteinModelPortal:   Q8K9B2
- SMR:   Q8K9B2
- EnsemblBacteria:   EBBUCT00000000090
- GeneID:   1005729
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg436
- GeneTree:   EBGT00050000007896
- HOGENOM:   HBG327581
- OMA:   RSAQTSM
- ProtClustDB:   PRK09197
- BioCyc:   BAPH198804:BUSG436-MONOMER
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01520

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 40638; Mature: 40638

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 110-110 BINDING 61-61 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILNLIQPGVVTGNECQIIFELAKKKKFAIPAVNCIGTDSINAVLETASRVKSPVIIQF
CCCHHHCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
SYGGASFIAGYKRTSSKNPEDQAIQGSISGAQHVHLMSQYYQIPVILHTDHCSKEMLPWI
ECCCHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
DGLLEEGKKHYKNFGKPLFTSHMIDLSKEHLKENIAICSNYLKKIKKINMILEIELGCTG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
GEEDGIDNSNIDKKLLYTQPKDVNYAYKKLSKISTNFTIAASFGNVHGVYQPGNVNLKPN
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCCH
ILKESQEYVSTKHNLKKLPLNLVFHGGSGSDLKEIHESIEYGIVKMNIDTDIQWASWKGV
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCEEHHHHHH
FNFYKKNKDFLQKQIGNKNGKNIPNKKYYDPRSWIRESQESMSKRLEKTFKDLNAFNIL
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILNLIQPGVVTGNECQIIFELAKKKKFAIPAVNCIGTDSINAVLETASRVKSPVIIQF
CCCHHHCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
SYGGASFIAGYKRTSSKNPEDQAIQGSISGAQHVHLMSQYYQIPVILHTDHCSKEMLPWI
ECCCHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
DGLLEEGKKHYKNFGKPLFTSHMIDLSKEHLKENIAICSNYLKKIKKINMILEIELGCTG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
GEEDGIDNSNIDKKLLYTQPKDVNYAYKKLSKISTNFTIAASFGNVHGVYQPGNVNLKPN
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCCH
ILKESQEYVSTKHNLKKLPLNLVFHGGSGSDLKEIHESIEYGIVKMNIDTDIQWASWKGV
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCEEHHHHHH
FNFYKKNKDFLQKQIGNKNGKNIPNKKYYDPRSWIRESQESMSKRLEKTFKDLNAFNIL
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438