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The map label for this gene is pgk

Identifier: 21672700

GI number: 21672700

Start: 491928

End: 493094

Strand: Direct

Name: pgk

Synonym: BUsg435

Alternate gene names: 21672700

Gene position: 491928-493094 (Clockwise)

Preceding gene: 21672699

Following gene: 21672701

Centisome position: 76.69

GC content: 26.99

Gene sequence:

>1167_bases
ATGAATTTAAGAAAAATGACTGAATTAAATATAACAGGAAAAAAAATTTTAATAAGAACAGATTTAAATGTTCCTATAAA
AAATGGCGTGATTCAATCTGATGCAAGAATACTTGCTGCTCTTCCTACCATTGAAATAGCTATTGAAAAAAAAGCTAAAA
TAATTATTTTATCTCATTTAGGACGTCCAAAAGAAGGGTGCTATACAAAAAAATATTCTTTATTTCCTATTTTTGAGTAT
CTAAAAAAGAAATTAAAAAAAACAAAAATTTACTTTTCTGATAATCACTTAAAAAAAGTTGAAATAAATTCAGGAGAAAT
ATTAATTTTAGAAAATGTTCGTTTTAACGTGGGAGAATTAAAAAACGATGAAAATTTATCTAAAGAATATGCTAATTTAT
GCGATATTTTTGTTATGGATGCTTTTGGTAGTGCACATAGAATGCAATCTTCAACATATGGCATTGGAAAATTTGTTAAA
ATTGCATGTGCAGGACCTCTTTTAATATCTGAAATTAACAACTTAAAAAAAGCGTTAAAAAATCCGAAACGTCCTATGGT
CGCTATAGTAGGAGGTGCAAAGGTATCAACCAAATTTAATGTGTTAAATAAATTAGCTGCAATTTCAGATACAATCATTG
TTGGAGGAGGTATAGCTAATACATTTTTAGCTATTGATAATAAAATTGGAAAATCGTTACATGAACCGAAATTTATACTA
AAAGCTAAAAAGTTACGTGATAAATACAACAACATTGTGATACCAATCGATTCTCGTGTAGGAAAAAACTTTTCTCATAA
AGAAAAATGCACAATAAAATTATCTTCTAATATTGAAAAAAACGAAGAAATCATGGATTTAGGTGATAAAACAATAAAAA
AAATTATCAAAATACTTAAAAAATCAAAAACAATTCTTTGGAATGGACCTGTTGGTGTTTTTGAGTTTCCTAATTTTCGT
AAAGGAACTGAAATGATTGCAAAAGCAATTGCAGAAAATAATGGATTTTCAATAGCTGGCGGTGGAGATACACTATCTGT
TATTGATATGTTTCAAATAAAAGATAAAATTTCTTATATTTCAACAGGAGGAGGAGCATTTTTAGAATTCATAGAAGGGA
AAAAATTACCTGCCATGAAAATGTTAGAAGAAAATTTTCACAAATAA

Upstream 100 bases:

>100_bases
ATTAATGGGTCTTATATTAATAATGCTTTCTACACAAATGTTTCTTGATGGAATAAAAGCATGGTTTAAAAATTAAAAAT
TATTAAATAAGGATGATTAT

Downstream 100 bases:

>100_bases
GTTTTAAACAATTCATTGTAATAAAAAATTCAATATAGCAGGAAAAAAAATGAAAATATTAAATCTTATTCAACCTGGAG
TTGTAACAGGTAATGAATGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNLRKMTELNITGKKILIRTDLNVPIKNGVIQSDARILAALPTIEIAIEKKAKIIILSHLGRPKEGCYTKKYSLFPIFEY
LKKKLKKTKIYFSDNHLKKVEINSGEILILENVRFNVGELKNDENLSKEYANLCDIFVMDAFGSAHRMQSSTYGIGKFVK
IACAGPLLISEINNLKKALKNPKRPMVAIVGGAKVSTKFNVLNKLAAISDTIIVGGGIANTFLAIDNKIGKSLHEPKFIL
KAKKLRDKYNNIVIPIDSRVGKNFSHKEKCTIKLSSNIEKNEEIMDLGDKTIKKIIKILKKSKTILWNGPVGVFEFPNFR
KGTEMIAKAIAENNGFSIAGGGDTLSVIDMFQIKDKISYISTGGGAFLEFIEGKKLPAMKMLEENFHK

Sequences:

>Translated_388_residues
MNLRKMTELNITGKKILIRTDLNVPIKNGVIQSDARILAALPTIEIAIEKKAKIIILSHLGRPKEGCYTKKYSLFPIFEY
LKKKLKKTKIYFSDNHLKKVEINSGEILILENVRFNVGELKNDENLSKEYANLCDIFVMDAFGSAHRMQSSTYGIGKFVK
IACAGPLLISEINNLKKALKNPKRPMVAIVGGAKVSTKFNVLNKLAAISDTIIVGGGIANTFLAIDNKIGKSLHEPKFIL
KAKKLRDKYNNIVIPIDSRVGKNFSHKEKCTIKLSSNIEKNEEIMDLGDKTIKKIIKILKKSKTILWNGPVGVFEFPNFR
KGTEMIAKAIAENNGFSIAGGGDTLSVIDMFQIKDKISYISTGGGAFLEFIEGKKLPAMKMLEENFHK
>Mature_388_residues
MNLRKMTELNITGKKILIRTDLNVPIKNGVIQSDARILAALPTIEIAIEKKAKIIILSHLGRPKEGCYTKKYSLFPIFEY
LKKKLKKTKIYFSDNHLKKVEINSGEILILENVRFNVGELKNDENLSKEYANLCDIFVMDAFGSAHRMQSSTYGIGKFVK
IACAGPLLISEINNLKKALKNPKRPMVAIVGGAKVSTKFNVLNKLAAISDTIIVGGGIANTFLAIDNKIGKSLHEPKFIL
KAKKLRDKYNNIVIPIDSRVGKNFSHKEKCTIKLSSNIEKNEEIMDLGDKTIKKIIKILKKSKTILWNGPVGVFEFPNFR
KGTEMIAKAIAENNGFSIAGGGDTLSVIDMFQIKDKISYISTGGGAFLEFIEGKKLPAMKMLEENFHK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=408, Percent_Identity=35.5392156862745, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=34.375, Blast_Score=218, Evalue=7e-57,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=58.0976863753213, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=37.0192307692308, Blast_Score=244, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=36.8932038834951, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=40, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24581203, Length=411, Percent_Identity=36.9829683698297, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BUCAP (Q8K9B3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660767.1
- ProteinModelPortal:   Q8K9B3
- SMR:   Q8K9B3
- EnsemblBacteria:   EBBUCT00000000520
- GeneID:   1005730
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg435
- GeneTree:   EBGT00050000007969
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   BAPH198804:BUSG435-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43248; Mature: 43248

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 198-198 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRKMTELNITGKKILIRTDLNVPIKNGVIQSDARILAALPTIEIAIEKKAKIIILSHL
CCCCCCEEEECCCCEEEEEECCCCCCCCCEECCCCEEEEECCEEEEEEECCCEEEEEECC
GRPKEGCYTKKYSLFPIFEYLKKKLKKTKIYFSDNHLKKVEINSGEILILENVRFNVGEL
CCCCCCCEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCEEEEECCCCCCCCC
KNDENLSKEYANLCDIFVMDAFGSAHRMQSSTYGIGKFVKIACAGPLLISEINNLKKALK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
NPKRPMVAIVGGAKVSTKFNVLNKLAAISDTIIVGGGIANTFLAIDNKIGKSLHEPKFIL
CCCCCEEEEECCCEECHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
KAKKLRDKYNNIVIPIDSRVGKNFSHKEKCTIKLSSNIEKNEEIMDLGDKTIKKIIKILK
HHHHHHHHHCCEEEEECHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHH
KSKTILWNGPVGVFEFPNFRKGTEMIAKAIAENNGFSIAGGGDTLSVIDMFQIKDKISYI
CCCEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHEE
STGGGAFLEFIEGKKLPAMKMLEENFHK
ECCCHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNLRKMTELNITGKKILIRTDLNVPIKNGVIQSDARILAALPTIEIAIEKKAKIIILSHL
CCCCCCEEEECCCCEEEEEECCCCCCCCCEECCCCEEEEECCEEEEEEECCCEEEEEECC
GRPKEGCYTKKYSLFPIFEYLKKKLKKTKIYFSDNHLKKVEINSGEILILENVRFNVGEL
CCCCCCCEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCEEEEECCCCCCCCC
KNDENLSKEYANLCDIFVMDAFGSAHRMQSSTYGIGKFVKIACAGPLLISEINNLKKALK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
NPKRPMVAIVGGAKVSTKFNVLNKLAAISDTIIVGGGIANTFLAIDNKIGKSLHEPKFIL
CCCCCEEEEECCCEECHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
KAKKLRDKYNNIVIPIDSRVGKNFSHKEKCTIKLSSNIEKNEEIMDLGDKTIKKIIKILK
HHHHHHHHHCCEEEEECHHHCCCCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHH
KSKTILWNGPVGVFEFPNFRKGTEMIAKAIAENNGFSIAGGGDTLSVIDMFQIKDKISYI
CCCEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHEE
STGGGAFLEFIEGKKLPAMKMLEENFHK
ECCCHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438