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The map label for this gene is holA

Identifier: 21672695

GI number: 21672695

Start: 487834

End: 488850

Strand: Direct

Name: holA

Synonym: BUsg430

Alternate gene names: 21672695

Gene position: 487834-488850 (Clockwise)

Preceding gene: 21672694

Following gene: 21672696

Centisome position: 76.05

GC content: 18.19

Gene sequence:

>1017_bases
ATGAAAATTATATACCCAGAAGAATTAAAACAAAAGTTAATTAAAAAATTAAATTATTTTTATATATTACTAGGAGAAGA
TTCAATTTTATTGAGTAAAGCTGAACAATTAATATTCTATTTTGCAAAAGATAAAAAATTTATAGAAAAAAATACTATTA
ATATAGAAAAAAGTACAGACTGGAACAAAGTTATTAATTTCCATAAATCAAAAAATTTATTTTTTAAAAAAACTATTTTA
ATCATTAATTTTACTATAAAAAAATTAAATTTATCTTTAGTTAAAAATATAAACAAAATACCTCTTTTTAAAAATAAAGA
TATACTTACAGTAATAAAATTGAATGCACTATCTAGTTTAATAAAAAAAAGTACAATATTAAAAATTCTGATTTTAGAAA
GCGAGATTATATATTGCTCCACTCCATATGAATGGGCATTTAAAAAATGGTTGCAATATGAAATAAAAACAAGAAATTTA
AAAATAACACAGGAATCTTTTTTATTATTACATAAATATTATGAAGGAAACACATTATGTTTAAATCAAATATTAGATAT
TCTTTCGATTACATGGCCTAATGAAAACATCAAAATAGAAAAAATAAAAAAAATCATTAATCAATTTTCAATTTTCTCTC
CTGTAAATTGGATAAATGCAATTTTTAATAAAAACAAAAAAAAAGCTATTTACATATTAGATAGTTTTTTTAAACAAAAA
TATAATCCTCTTATTTTAATACGAACCTTGCAAAAAGATCTATTGATTTTACTAAACATGAAAAGAGAAAAAAAATTAAA
TATAAATTTTTTCTTAAAAAAAAATAATATTTCGTTGAATAGATCAAAATTTTTTGCTAATGCGCTGTATTATATTGATT
TTAATAATTTTTTAAAAATAATAAGAATCTTATTGCAAATAGAAATAAAAATAAAAAAAGAATATAATAATTCTGTATGG
ATTCAATTAAAAACACTAACATTATTACTATCTTCACCAATGAAATATACACCGTAA

Upstream 100 bases:

>100_bases
TAAAAAATTTAAAAATAATTAAAATAATTTATATTCCTCAAAAAGTGATTAACTTTGTAGTATAAACCTAAGAGATCATA
AAAGTGATATTTTTATTCAA

Downstream 100 bases:

>100_bases
AAACATGAAAGAAATATATGCAATATTTGGAGGGAATTTTGATCCTATTCATTATGGTCATATTACTTCTGCTGAAAAAT
TATCGCGCGAAATTTCAATA

Product: DNA polymerase III delta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKIIYPEELKQKLIKKLNYFYILLGEDSILLSKAEQLIFYFAKDKKFIEKNTINIEKSTDWNKVINFHKSKNLFFKKTIL
IINFTIKKLNLSLVKNINKIPLFKNKDILTVIKLNALSSLIKKSTILKILILESEIIYCSTPYEWAFKKWLQYEIKTRNL
KITQESFLLLHKYYEGNTLCLNQILDILSITWPNENIKIEKIKKIINQFSIFSPVNWINAIFNKNKKKAIYILDSFFKQK
YNPLILIRTLQKDLLILLNMKREKKLNINFFLKKNNISLNRSKFFANALYYIDFNNFLKIIRILLQIEIKIKKEYNNSVW
IQLKTLTLLLSSPMKYTP

Sequences:

>Translated_338_residues
MKIIYPEELKQKLIKKLNYFYILLGEDSILLSKAEQLIFYFAKDKKFIEKNTINIEKSTDWNKVINFHKSKNLFFKKTIL
IINFTIKKLNLSLVKNINKIPLFKNKDILTVIKLNALSSLIKKSTILKILILESEIIYCSTPYEWAFKKWLQYEIKTRNL
KITQESFLLLHKYYEGNTLCLNQILDILSITWPNENIKIEKIKKIINQFSIFSPVNWINAIFNKNKKKAIYILDSFFKQK
YNPLILIRTLQKDLLILLNMKREKKLNINFFLKKNNISLNRSKFFANALYYIDFNNFLKIIRILLQIEIKIKKEYNNSVW
IQLKTLTLLLSSPMKYTP
>Mature_338_residues
MKIIYPEELKQKLIKKLNYFYILLGEDSILLSKAEQLIFYFAKDKKFIEKNTINIEKSTDWNKVINFHKSKNLFFKKTIL
IINFTIKKLNLSLVKNINKIPLFKNKDILTVIKLNALSSLIKKSTILKILILESEIIYCSTPYEWAFKKWLQYEIKTRNL
KITQESFLLLHKYYEGNTLCLNQILDILSITWPNENIKIEKIKKIINQFSIFSPVNWINAIFNKNKKKAIYILDSFFKQK
YNPLILIRTLQKDLLILLNMKREKKLNINFFLKKNNISLNRSKFFANALYYIDFNNFLKIIRILLQIEIKIKKEYNNSVW
IQLKTLTLLLSSPMKYTP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=329, Percent_Identity=27.9635258358663, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLA_BUCAP (Q8K9B8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660762.1
- ProteinModelPortal:   Q8K9B8
- SMR:   Q8K9B8
- EnsemblBacteria:   EBBUCT00000000209
- GeneID:   1005733
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg430
- GeneTree:   EBGT00050000007725
- HOGENOM:   HBG366221
- OMA:   NHWIESI
- ProtClustDB:   CLSK316213
- BioCyc:   BAPH198804:BUSG430-MONOMER
- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790
- TIGRFAMs:   TIGR01128

Pfam domain/function: PF06144 DNA_pol3_delta; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 40430; Mature: 40430

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIYPEELKQKLIKKLNYFYILLGEDSILLSKAEQLIFYFAKDKKFIEKNTINIEKSTD
CCEECHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHEEEEEECCCCEEECCEEEEECCCC
WNKVINFHKSKNLFFKKTILIINFTIKKLNLSLVKNINKIPLFKNKDILTVIKLNALSSL
HHHHHHHHCCCCEEEEEEEEEEEEEEEHCCHHHHCCCCCCCCCCCCCEEEEEEHHHHHHH
IKKSTILKILILESEIIYCSTPYEWAFKKWLQYEIKTRNLKITQESFLLLHKYYEGNTLC
HHHHHEEEEEEEECCEEEEECCHHHHHHHHHHEEEEECEEEEEHHHEEEEEEECCCCEEE
LNQILDILSITWPNENIKIEKIKKIINQFSIFSPVNWINAIFNKNKKKAIYILDSFFKQK
HHHHHHHHEEECCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEHHHHCC
YNPLILIRTLQKDLLILLNMKREKKLNINFFLKKNNISLNRSKFFANALYYIDFNNFLKI
CCCEEEEEEHHHHHEEEEECCCCCEEEEEEEEEECCCCCCHHHHEEEEEEEEEHHHHHHH
IRILLQIEIKIKKEYNNSVWIQLKTLTLLLSSPMKYTP
HHHHHHEEEEEEEECCCEEEEEEEEEEHHHCCCCCCCC
>Mature Secondary Structure
MKIIYPEELKQKLIKKLNYFYILLGEDSILLSKAEQLIFYFAKDKKFIEKNTINIEKSTD
CCEECHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHEEEEEECCCCEEECCEEEEECCCC
WNKVINFHKSKNLFFKKTILIINFTIKKLNLSLVKNINKIPLFKNKDILTVIKLNALSSL
HHHHHHHHCCCCEEEEEEEEEEEEEEEHCCHHHHCCCCCCCCCCCCCEEEEEEHHHHHHH
IKKSTILKILILESEIIYCSTPYEWAFKKWLQYEIKTRNLKITQESFLLLHKYYEGNTLC
HHHHHEEEEEEEECCEEEEECCHHHHHHHHHHEEEEECEEEEEHHHEEEEEEECCCCEEE
LNQILDILSITWPNENIKIEKIKKIINQFSIFSPVNWINAIFNKNKKKAIYILDSFFKQK
HHHHHHHHEEECCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEHHHHCC
YNPLILIRTLQKDLLILLNMKREKKLNINFFLKKNNISLNRSKFFANALYYIDFNNFLKI
CCCEEEEEEHHHHHEEEEECCCCCEEEEEEEEEECCCCCCHHHHEEEEEEEEEHHHHHHH
IRILLQIEIKIKKEYNNSVWIQLKTLTLLLSSPMKYTP
HHHHHHEEEEEEEECCCEEEEEEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438