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The map label for this gene is thyA

Identifier: 21672690

GI number: 21672690

Start: 481374

End: 482168

Strand: Direct

Name: thyA

Synonym: BUsg425

Alternate gene names: 21672690

Gene position: 481374-482168 (Clockwise)

Preceding gene: 21672689

Following gene: 21672691

Centisome position: 75.04

GC content: 26.54

Gene sequence:

>795_bases
ATGAAACAATATTTAGATTTAATAAAAAAAATAATTAAAAATGGAAATTCAAAAAAAGATCGAACCGGTACAGGAACTTT
ATCTATTTTTGGACATCATATGAAATTTGATTTAAAAAAAGGTTTTCCTCTTATCACAACAAAAAAATGTCATATACCAT
CAATTATTCATGAACTTTTATGGTTTCTTAGGGGAGAAACAAATATTAAATATCTTAACGAAAATAAAATATCAATTTGG
GATAACTGGGCAGATAAATTTGGTGATCTAGGTCCAATTTATGGAAAACAATGGAGAAGTTGGGATACATCTAACGGAAA
AAAAATCGATCAAATAAAAAACGTACTAAAACAAATAAAAGAAGATCCTGATTCACGTAGAATTTTAGTATCTAGTTGGA
ACGTTGGAGAAATTGATAAAATGTCTTTAGCTCCATGTCATATATTATTTCAATTTTACGTATTAAAAAAAAAATTAAGT
TGTCAACTTTACCAACGTTCTTGCGATGTATTTCTTGGTTTGCCTTTTAATATAGCTAGCTATGCAATACTGATACATAT
GATAGCTCAACAATGTAATCTACAAGTTGGAGAATTTTTATGGACAGGAGGTGACGTTCATTTATATAAAAATCATGTTG
AATTGGCGAAAAAACAAATATTGAGAATACCTAAAAAATTACCAGAGTTAATAATTTTAAAAAAACCTCAATCATTATTT
CAATATTCTTTTGAAGATTTTAAAATTGTTGGGTATAAACCATATTCCTTAATTAAGGGAAAAATATCTGTATAA

Upstream 100 bases:

>100_bases
AATAACTATGGGTCAAATATTGTCTATTCCGATGATTATTATTGGATGTATTATAATATGTAAATCTATTTATAAAAAAT
AATTCTTTTATTGAGGCAGT

Downstream 100 bases:

>100_bases
AAATACGATATTTTTACATAAAAGTAGGATTAATTTTACTAAAATGAAATATATATATATAAAAACTTGGGGTTGTCAAA
TGAATGAATATGATTCATCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLIKKIIKNGNSKKDRTGTGTLSIFGHHMKFDLKKGFPLITTKKCHIPSIIHELLWFLRGETNIKYLNENKISIW
DNWADKFGDLGPIYGKQWRSWDTSNGKKIDQIKNVLKQIKEDPDSRRILVSSWNVGEIDKMSLAPCHILFQFYVLKKKLS
CQLYQRSCDVFLGLPFNIASYAILIHMIAQQCNLQVGEFLWTGGDVHLYKNHVELAKKQILRIPKKLPELIILKKPQSLF
QYSFEDFKIVGYKPYSLIKGKISV

Sequences:

>Translated_264_residues
MKQYLDLIKKIIKNGNSKKDRTGTGTLSIFGHHMKFDLKKGFPLITTKKCHIPSIIHELLWFLRGETNIKYLNENKISIW
DNWADKFGDLGPIYGKQWRSWDTSNGKKIDQIKNVLKQIKEDPDSRRILVSSWNVGEIDKMSLAPCHILFQFYVLKKKLS
CQLYQRSCDVFLGLPFNIASYAILIHMIAQQCNLQVGEFLWTGGDVHLYKNHVELAKKQILRIPKKLPELIILKKPQSLF
QYSFEDFKIVGYKPYSLIKGKISV
>Mature_264_residues
MKQYLDLIKKIIKNGNSKKDRTGTGTLSIFGHHMKFDLKKGFPLITTKKCHIPSIIHELLWFLRGETNIKYLNENKISIW
DNWADKFGDLGPIYGKQWRSWDTSNGKKIDQIKNVLKQIKEDPDSRRILVSSWNVGEIDKMSLAPCHILFQFYVLKKKLS
CQLYQRSCDVFLGLPFNIASYAILIHMIAQQCNLQVGEFLWTGGDVHLYKNHVELAKKQILRIPKKLPELIILKKPQSLF
QYSFEDFKIVGYKPYSLIKGKISV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=45.0354609929078, Blast_Score=254, Evalue=6e-68,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=66.6666666666667, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=40.9893992932862, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324648, Length=280, Percent_Identity=39.6428571428571, Blast_Score=192, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=47.1830985915493, Blast_Score=271, Evalue=3e-73,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_BUCAP (Q8K9C3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660757.1
- ProteinModelPortal:   Q8K9C3
- SMR:   Q8K9C3
- EnsemblBacteria:   EBBUCT00000000167
- GeneID:   1005738
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg425
- GeneTree:   EBGT00050000008191
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   BAPH198804:BUSG425-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30717; Mature: 30717

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLIKKIIKNGNSKKDRTGTGTLSIFGHHMKFDLKKGFPLITTKKCHIPSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEECCCCCCEEECCCCCCHHHHHHHH
WFLRGETNIKYLNENKISIWDNWADKFGDLGPIYGKQWRSWDTSNGKKIDQIKNVLKQIK
HHHCCCCCEEEECCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EDPDSRRILVSSWNVGEIDKMSLAPCHILFQFYVLKKKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
YAILIHMIAQQCNLQVGEFLWTGGDVHLYKNHVELAKKQILRIPKKLPELIILKKPQSLF
HHHHHHHHHHHHCCCHHHEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
QYSFEDFKIVGYKPYSLIKGKISV
HCCCCCEEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MKQYLDLIKKIIKNGNSKKDRTGTGTLSIFGHHMKFDLKKGFPLITTKKCHIPSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEECCCCCCEEECCCCCCHHHHHHHH
WFLRGETNIKYLNENKISIWDNWADKFGDLGPIYGKQWRSWDTSNGKKIDQIKNVLKQIK
HHHCCCCCEEEECCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EDPDSRRILVSSWNVGEIDKMSLAPCHILFQFYVLKKKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
YAILIHMIAQQCNLQVGEFLWTGGDVHLYKNHVELAKKQILRIPKKLPELIILKKPQSLF
HHHHHHHHHHHHCCCHHHEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
QYSFEDFKIVGYKPYSLIKGKISV
HCCCCCEEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438