Click here to switch to the map view.

The map label for this gene is yleA

Identifier: 21672691

GI number: 21672691

Start: 482212

End: 483531

Strand: Direct

Name: yleA

Synonym: BUsg426

Alternate gene names: 21672691

Gene position: 482212-483531 (Clockwise)

Preceding gene: 21672690

Following gene: 21672692

Centisome position: 75.17

GC content: 26.67

Gene sequence:

>1320_bases
ATGAAATATATATATATAAAAACTTGGGGTTGTCAAATGAATGAATATGATTCATCTATGATAACGGTATTTTTAGAGAA
AACAGGAAGATATTTAATTACTAAAGAAGTTGAAAAAGCAGATATATTAATTTTAAATACTTGTTCTATAAGAGAAAAAG
CTCAAGAAAAGGTTTTTCATCAATTAGGAAGATGGAAAAAATTAAAAAATAAAAAACCTGATGTTATTATTGCCGTAGGA
GGTTGTGTAGCAACACAAGAAGGTAAAGAAATTTTTAAAAGAGCAAACTATGTAGATATTGTATTTGGTACACAAACTTT
ACATAAACTAGCTCAAATGATTGATAAAGTAGAAAAAAATCGTCGATTTATGATTGATATTAGTTTTCCTCAATTAGAAA
AATTTAATTATTCTTTAGAACCTAAAAAAACAGGATATACAGCTTCTATTTCTATCATGGAGGGATGTAATAAATACTGT
TCATTTTGTGTTGTACCATATACTAGAGGACATGAAATCAGTCGTCCATCTGATGATATTTTGTTAGAAATATCAATCTT
AGCTGAAAACGGTGTAAGAGAAATTAATTTATTAGGACAAAATGTTAATGCATATCGAGGTCGAACGTTTAATGGAAAAA
TTTGTTTTTTCTCAGAATTATTGCGATTAGTTTCTGAAATAGATGGTATCGATAGAATTCGTTTTACAACAAGTAATCCA
CTTGAATTTACTGATGACATTATTGAAGTTTATCAAGATACACCAAAATTAGTGAGCTTTCTCCATCTTCCTGTACAAAG
TGGATCAAATAGAATCCTACAACTAATGAAAAGATCGTATACAACAGAAGAATACGAAAAAATCATCGAAAAACTTATTT
TAGCTCGTTCAAATATTCAAATTAGTTCTGATTTTATTGTTGGATTTCCTAGTGAATCTGAAGAAGATTTTCAAAAAACT
ATTAATTTTATAAAAAAAATAAATTTTGATATGAGTTTTAGTTTTATATATTCTAGTCGACCAGGTACACCAGCTTCAGA
AATGAAAGATAATATTGATTTACAAGAGAAAAAAAAACGCTTATATACTTTACAGAATTGTATCAATAAGCAAACTATGT
CTTGGAGTAGAAAAATGTTAAAAAGCACACAATCTGTTTTAGTAGAAGGTGTATCAAAAAAAAACATTATGGAATTATAT
GGAAAAACAGAAAACAATAGAATTGTTACTTTTAAAGGATCGCATAAGATGATTGGTAAATTTATTAAGTTAAAAATTAA
AAAGGTACATACTCATTCATTACAAGGTGAATTAATTTAA

Upstream 100 bases:

>100_bases
TTTAAAATTGTTGGGTATAAACCATATTCCTTAATTAAGGGAAAAATATCTGTATAAAAATACGATATTTTTACATAAAA
GTAGGATTAATTTTACTAAA

Downstream 100 bases:

>100_bases
AATTAAATGAAAAAAAAAAATAACATTATTTTAACAATTCAAAAAAATTGTAAAAACACTAAAAATATACCAAAAAAATA
TATTTTTGAAAAATGGATAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKYIYIKTWGCQMNEYDSSMITVFLEKTGRYLITKEVEKADILILNTCSIREKAQEKVFHQLGRWKKLKNKKPDVIIAVG
GCVATQEGKEIFKRANYVDIVFGTQTLHKLAQMIDKVEKNRRFMIDISFPQLEKFNYSLEPKKTGYTASISIMEGCNKYC
SFCVVPYTRGHEISRPSDDILLEISILAENGVREINLLGQNVNAYRGRTFNGKICFFSELLRLVSEIDGIDRIRFTTSNP
LEFTDDIIEVYQDTPKLVSFLHLPVQSGSNRILQLMKRSYTTEEYEKIIEKLILARSNIQISSDFIVGFPSESEEDFQKT
INFIKKINFDMSFSFIYSSRPGTPASEMKDNIDLQEKKKRLYTLQNCINKQTMSWSRKMLKSTQSVLVEGVSKKNIMELY
GKTENNRIVTFKGSHKMIGKFIKLKIKKVHTHSLQGELI

Sequences:

>Translated_439_residues
MKYIYIKTWGCQMNEYDSSMITVFLEKTGRYLITKEVEKADILILNTCSIREKAQEKVFHQLGRWKKLKNKKPDVIIAVG
GCVATQEGKEIFKRANYVDIVFGTQTLHKLAQMIDKVEKNRRFMIDISFPQLEKFNYSLEPKKTGYTASISIMEGCNKYC
SFCVVPYTRGHEISRPSDDILLEISILAENGVREINLLGQNVNAYRGRTFNGKICFFSELLRLVSEIDGIDRIRFTTSNP
LEFTDDIIEVYQDTPKLVSFLHLPVQSGSNRILQLMKRSYTTEEYEKIIEKLILARSNIQISSDFIVGFPSESEEDFQKT
INFIKKINFDMSFSFIYSSRPGTPASEMKDNIDLQEKKKRLYTLQNCINKQTMSWSRKMLKSTQSVLVEGVSKKNIMELY
GKTENNRIVTFKGSHKMIGKFIKLKIKKVHTHSLQGELI
>Mature_439_residues
MKYIYIKTWGCQMNEYDSSMITVFLEKTGRYLITKEVEKADILILNTCSIREKAQEKVFHQLGRWKKLKNKKPDVIIAVG
GCVATQEGKEIFKRANYVDIVFGTQTLHKLAQMIDKVEKNRRFMIDISFPQLEKFNYSLEPKKTGYTASISIMEGCNKYC
SFCVVPYTRGHEISRPSDDILLEISILAENGVREINLLGQNVNAYRGRTFNGKICFFSELLRLVSEIDGIDRIRFTTSNP
LEFTDDIIEVYQDTPKLVSFLHLPVQSGSNRILQLMKRSYTTEEYEKIIEKLILARSNIQISSDFIVGFPSESEEDFQKT
INFIKKINFDMSFSFIYSSRPGTPASEMKDNIDLQEKKKRLYTLQNCINKQTMSWSRKMLKSTQSVLVEGVSKKNIMELY
GKTENNRIVTFKGSHKMIGKFIKLKIKKVHTHSLQGELI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=31.8658280922432, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=31.8658280922432, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI93277076, Length=375, Percent_Identity=28.2666666666667, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1786882, Length=438, Percent_Identity=64.6118721461187, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=26.8888888888889, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=29.8924731182796, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=289, Percent_Identity=28.719723183391, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=30.5263157894737, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=27.3522975929978, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BUCAP (Q8K9C2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660758.1
- ProteinModelPortal:   Q8K9C2
- SMR:   Q8K9C2
- EnsemblBacteria:   EBBUCT00000000269
- GeneID:   1005737
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg426
- GeneTree:   EBGT00050000007978
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   BAPH198804:BUSG426-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50788; Mature: 50788

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIYIKTWGCQMNEYDSSMITVFLEKTGRYLITKEVEKADILILNTCSIREKAQEKVFH
CEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHH
QLGRWKKLKNKKPDVIIAVGGCVATQEGKEIFKRANYVDIVFGTQTLHKLAQMIDKVEKN
HHHHHHHHCCCCCCEEEEECCEEECHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCC
RRFMIDISFPQLEKFNYSLEPKKTGYTASISIMEGCNKYCSFCVVPYTRGHEISRPSDDI
CEEEEEECCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCE
LLEISILAENGVREINLLGQNVNAYRGRTFNGKICFFSELLRLVSEIDGIDRIRFTTSNP
EEEEEEEECCCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
LEFTDDIIEVYQDTPKLVSFLHLPVQSGSNRILQLMKRSYTTEEYEKIIEKLILARSNIQ
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ISSDFIVGFPSESEEDFQKTINFIKKINFDMSFSFIYSSRPGTPASEMKDNIDLQEKKKR
EECCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHH
LYTLQNCINKQTMSWSRKMLKSTQSVLVEGVSKKNIMELYGKTENNRIVTFKGSHKMIGK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHH
FIKLKIKKVHTHSLQGELI
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYIYIKTWGCQMNEYDSSMITVFLEKTGRYLITKEVEKADILILNTCSIREKAQEKVFH
CEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHH
QLGRWKKLKNKKPDVIIAVGGCVATQEGKEIFKRANYVDIVFGTQTLHKLAQMIDKVEKN
HHHHHHHHCCCCCCEEEEECCEEECHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCC
RRFMIDISFPQLEKFNYSLEPKKTGYTASISIMEGCNKYCSFCVVPYTRGHEISRPSDDI
CEEEEEECCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCE
LLEISILAENGVREINLLGQNVNAYRGRTFNGKICFFSELLRLVSEIDGIDRIRFTTSNP
EEEEEEEECCCCEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
LEFTDDIIEVYQDTPKLVSFLHLPVQSGSNRILQLMKRSYTTEEYEKIIEKLILARSNIQ
CHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
ISSDFIVGFPSESEEDFQKTINFIKKINFDMSFSFIYSSRPGTPASEMKDNIDLQEKKKR
EECCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHH
LYTLQNCINKQTMSWSRKMLKSTQSVLVEGVSKKNIMELYGKTENNRIVTFKGSHKMIGK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHHHHH
FIKLKIKKVHTHSLQGELI
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438